Gel Electrophoresis Quiz
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Questions and Answers

What is the main factor influencing the rate of movement for DNA through the agarose gel in gel electrophoresis?

  • The strength of the electrical field (voltage)
  • The concentration of agarose in the gel
  • The size of the DNA molecules (correct)
  • The presence of phosphate in the DNA structure

What is the function of the standard molecular-weight markers (Ladder) in gel electrophoresis?

  • To calculate the size of the separated DNA fragments (correct)
  • To neutralize the negative charge of DNA
  • To determine the concentration of agarose in the gel
  • To provide a positive charge for DNA migration

What is the net charge of all nucleic acids in DNA molecules?

  • Net negative charge (correct)
  • Neutral charge
  • Variable charge based on size
  • Net positive charge

What is the main component used for the separation of DNA and RNA fragments in agarose gel electrophoresis?

<p>Agarose (B)</p> Signup and view all the answers

Which factor influences the fast movement of DNA through the agarose gel in gel electrophoresis?

<p>High voltage (C)</p> Signup and view all the answers

What is the main component used for the separation of DNA and RNA fragments in agarose gel electrophoresis?

<p>Agarose (C)</p> Signup and view all the answers

What is the function of the standard molecular-weight markers (Ladder) in gel electrophoresis?

<p>To estimate the size of separated DNA fragments (A)</p> Signup and view all the answers

What factor influences the fast movement of DNA through the agarose gel in gel electrophoresis?

<p>The size of the DNA molecules (C)</p> Signup and view all the answers

What is the net charge of all nucleic acids in DNA molecules?

<p>Net negative charge (A)</p> Signup and view all the answers

What type of gel is specifically used for the separation of DNA and RNA fragments in gel electrophoresis?

<p>Agarose Gel (B)</p> Signup and view all the answers

Flashcards

DNA size in gel electrophoresis

Larger DNA fragments move slower, smaller ones faster, through an agarose gel during electrophoresis.

Molecular Weight Markers (Ladder)

Used as a reference to determine the size of unknown DNA fragments in gel electrophoresis.

Nucleic Acid Charge

DNA and RNA have a net negative electric charge.

Agarose in Electrophoresis

A substance used to separate DNA and RNA fragments by size in a gel matrix.

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High Voltage in Electrophoresis

Increases the rate of DNA movement through the gel.

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DNA fragments separation

DNA molecules are separated based on their size in agarose gel electrophoresis

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Gel Electrophoresis

A technique to separate DNA or RNA fragments by size using an agarose gel and electrical current.

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DNA Size and Electrophoresis Speed

Smaller DNA fragments move faster through the gel compared to larger ones.

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DNA Charge and Gel Electrophoresis

Due to the negative charge of nucleic acids, DNA fragments migrate towards the positive electrode during electrophoresis.

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Agarose Gel in Electrophoresis

Used as a medium for separating DNA or RNA fragments by size under the influence of applied electrical current.

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Study Notes

Factors Influencing DNA Movement in Gel Electrophoresis

  • The main factor influencing the rate of movement for DNA through the agarose gel is the size of the DNA molecule, with smaller DNA molecules moving faster than larger ones.
  • The fast movement of DNA through the agarose gel is influenced by the size of the DNA molecule, with smaller molecules moving faster.

Function of Standard Molecular-Weight Markers (Ladder)

  • The function of the standard molecular-weight markers (Ladder) in gel electrophoresis is to provide a set of DNA molecules of known sizes, allowing for the estimation of the size of unknown DNA molecules.

Net Charge of Nucleic Acids

  • The net charge of all nucleic acids in DNA molecules is negative due to the phosphate groups.

Main Component for Separation of DNA and RNA Fragments

  • The main component used for the separation of DNA and RNA fragments in agarose gel electrophoresis is agarose.

Type of Gel Used for Separation

  • Agarose gel is specifically used for the separation of DNA and RNA fragments in gel electrophoresis.

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Description

Test your knowledge of gel electrophoresis with this quiz. Explore the principles of DNA separation based on charge and size, and understand the technique's role in analyzing nucleic acids.

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