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Questions and Answers
Which technique is commonly used to separate DNA fragments based on their size?
Which technique is commonly used to separate DNA fragments based on their size?
- Gel electrophoresis (correct)
- Polymerase chain reaction (PCR)
- Southern blotting
- Western blotting
What is the purpose of gel electrophoresis?
What is the purpose of gel electrophoresis?
- To amplify DNA fragments
- To visualize protein expression
- To separate DNA fragments based on size (correct)
- To analyze gene expression
Which of the following is a key component of gel electrophoresis?
Which of the following is a key component of gel electrophoresis?
- RNA transcriptase
- Agarose gel (correct)
- DNA polymerase
- Antibodies
Which of the following is a key step in gel electrophoresis?
Which of the following is a key step in gel electrophoresis?
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What is the purpose of reading gel electrophoresis results?
What is the purpose of reading gel electrophoresis results?
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Which of the following is true about restriction enzymes?
Which of the following is true about restriction enzymes?
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Study Notes
Gel Electrophoresis
- Agarose gel electrophoresis is a technique commonly used to separate DNA fragments based on their size.
- The purpose of gel electrophoresis is to analyze and separate DNA fragments according to their size and charge.
- A key component of gel electrophoresis is agarose, a polysaccharide derived from seaweed that forms a gel-like matrix when dissolved in water.
- A key step in gel electrophoresis is the application of an electric current, which causes the DNA fragments to migrate through the agarose gel at different rates based on their size and charge.
Restriction Enzymes
- Restriction enzymes are true biological scissors that cut DNA at specific recognition sites, producing DNA fragments of specific sizes.
- The purpose of reading gel electrophoresis results is to identify the size and number of DNA fragments produced by restriction enzymes, which can be used to create a genetic map or identify specific DNA sequences.
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