Microbiology Quiz on Identification Tests
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Questions and Answers

What indicates a positive coagulase test result in the slide and tube tests?

  • A color change to dark blue
  • No clumping observed
  • Bubbles forming upon adding Hydrogen Peroxide
  • Clumping observed (correct)
  • Which component is used in the Oxidase Test as a final electron acceptor?

  • N, N, N, N-Tetramethyl Phenylenediamine Dihydrochloride (correct)
  • Ferrous sulfate
  • Oxygen
  • Nitrogen
  • What is the purpose of bile salts in Bile Esculin Agar?

  • To selectively restrict the growth of some gram-positive organisms (correct)
  • To act as an energy source for microorganisms
  • To promote the growth of all gram-positive organisms
  • To inhibit the growth of specific gram-negative organisms
  • What is indicated by bubbling when bacteria are added to Hydrogen Peroxide?

    <p>The bacteria are catalase positive</p> Signup and view all the answers

    Which of the following statements about Esculin is true?

    <p>Esculin hydrolysis leads to a loss of fluorescence in the medium</p> Signup and view all the answers

    What color indicates a positive result in the Oxidase test?

    <p>Purple</p> Signup and view all the answers

    Which reagent is essential for performing the Gram Stain?

    <p>Acetic Alcohol</p> Signup and view all the answers

    What is the incubation temperature for the Bile Esculin test?

    <p>37 degrees Celsius</p> Signup and view all the answers

    In the Slide & Tube Coagulase test, what material is mixed with the bacterium culture?

    <p>Rabbit Plasma</p> Signup and view all the answers

    Which step is performed last when conducting a Gram Stain?

    <p>Application of Safranine</p> Signup and view all the answers

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