Microbiology Identification Tests
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Questions and Answers

What does a positive DNase test indicate?

  • Hydrolysis of the medium (correct)
  • Clear zone around the organism
  • Growth without fermentation
  • Presence of pink color
  • In the Mannitol Fermentation Test, what color indicates growth with fermentation?

  • Clear zone
  • Orange color
  • Yellow halos (correct)
  • Pink to red
  • What is the result of a Novobiocin Susceptibility Test for S.epidermidis?

  • Zone diameter less than or equal to 16 mm
  • Zone diameter greater than 16 mm (correct)
  • Yellow halos surrounding growth
  • No clearing observed
  • Which organism shows no color change or an orange color in the PYR Test?

    <p>S.aureus</p> Signup and view all the answers

    What does a negative catalase test result indicate?

    <p>Negative for catalase production</p> Signup and view all the answers

    What is the characteristic result of S.aureus in the Coagulase Test?

    <p>Positive coagulase activity</p> Signup and view all the answers

    What is the main purpose of the Catalase Test described in the text?

    <p>To test the breakdown of H2O2 into oxygen and water</p> Signup and view all the answers

    Which type of bacteria would most likely produce macroscopic clumping in the Coagulase Slide Test?

    <p>Staphylococcus aureus</p> Signup and view all the answers

    What is the result of the Coagulase Tube Test if no clot is formed?

    <p>Negative for free coagulase</p> Signup and view all the answers

    Which test detects the resistance to 0.04U Bacitracin?

    <p>Novobiocin Susceptibility Test</p> Signup and view all the answers

    What does a positive result in the DNAse Test suggest?

    <p>Presence of nuclease activity</p> Signup and view all the answers

    In the Mannitol Fermentation Test, what does a change in pH due to fermentation typically result in?

    <p>Yellow color change of the medium</p> Signup and view all the answers

    What is the purpose of using Colistin-Nalidixic Agar or Phenylethyl Alcohol Agar as mentioned in the text?

    <p>To selectively isolate Staphylococci</p> Signup and view all the answers

    Which organism shows total inhibition on Colistin-Nalidixic Agar or Phenylethyl Alcohol Agar?

    <p>E.coli</p> Signup and view all the answers

    What does a positive catalase test indicate?

    <p>Presence of Staphylococcus</p> Signup and view all the answers

    In the Novobiocin susceptibility test, which organism is resistant?

    <p>S.epidermidis</p> Signup and view all the answers

    What is the key characteristic of Mannitol Salt Agar?

    <p>Differential for Staphylococcus based on mannitol fermentation</p> Signup and view all the answers

    Which test can differentiate between Staphylococcus aureus and Staphylococcus epidermidis?

    <p>Coagulase test</p> Signup and view all the answers

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