Microbiology Lab 2 Sterility Testing with Prof. Yasser Gaber

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18 Questions

What is the purpose of labeling 6 pairs of screw-capped test tubes in the experimental setup?

To identify the tubes for Sterile Control, Microbial Growth Control, and Pharmaceutical Sterility Evaluation

Why is it important for students to work in a laminar airflow cabinet or an aseptic area during the experiment?

To practice inoculation under sterile conditions

What does FTM stand for in the context of the experiment?

Fluid Thioglycollate Medium

Which of the following is NOT a purpose of TSB mentioned in the text?

Biochemical characterization of fungal isolates

In a sterility testing experiment, what is the significance of the Sterile Control tube?

It ensures that no contamination occurs during handling

Why is it necessary to record the initial color of the FTM medium before starting the experiment?

To observe any color changes that may indicate bacterial growth

What is the objective of the experiment described in the text?

To evaluate the effectiveness of pharmaceutical sterilization methods

Which medium is NOT mentioned as a growth medium used in the experiment?

MacConkey Agar

What type of organisms are Escherichia coli and Saccharomyces cerevisiae in the context of the experiment?

Non-pathogenic bacteria

Which material is NOT listed in the 'Materials' section for the experiment?

Nutrient Broth

What is the purpose of using Tryptic Soy Broth (TSB) in the experiment?

To test for fermentative properties of microorganisms

In the context of sterility testing, what role does Fluid Thioglycollate Medium (FTM) play?

Detecting anaerobic microorganisms

What is the recommended incubation period for thorough evaluation of sterility in the tubes?

7 days

What should be done if any signs of microbial growth are observed during the incubation period?

Record the observation

What does the absence of growth in the test sample indicate?

Successful sterilization

Which action confirms the viability of the media and the experimental setup during sterility testing?

Growth in the positive control

What precaution is emphasized for handling microbial cultures during the sterility testing procedure?

Wearing appropriate PPE

What should be done if turbidity is observed in one of the tubes during observation after incubation?

Discard the tube immediately

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