Microbiology Lab 2 Sterility Testing with Prof. Yasser Gaber

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What is the purpose of labeling 6 pairs of screw-capped test tubes in the experimental setup?

To distinguish between different types of bacteria

To show the color changes in the FTM medium

To identify the tubes for Sterile Control, Microbial Growth Control, and Pharmaceutical Sterility Evaluation (correct)

To indicate different levels of bacterial growth

Why is it important for students to work in a laminar airflow cabinet or an aseptic area during the experiment?

To speed up the growth of bacteria

To observe the color changes in the FTM medium

To differentiate between aerobic and anaerobic bacteria

To practice inoculation under sterile conditions (correct)

What does FTM stand for in the context of the experiment?

Fungal Transmission Medium

Fluid Thioglycollate Medium (correct)

Facultative Total Medium

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Which of the following is NOT a purpose of TSB mentioned in the text?

Biochemical characterization of fungal isolates Signup and view all the answers

In a sterility testing experiment, what is the significance of the Sterile Control tube?

It ensures that no contamination occurs during handling Signup and view all the answers

Why is it necessary to record the initial color of the FTM medium before starting the experiment?

To observe any color changes that may indicate bacterial growth Signup and view all the answers

What is the objective of the experiment described in the text?

To evaluate the effectiveness of pharmaceutical sterilization methods Signup and view all the answers

Which medium is NOT mentioned as a growth medium used in the experiment?

MacConkey Agar Signup and view all the answers

What type of organisms are Escherichia coli and Saccharomyces cerevisiae in the context of the experiment?

Non-pathogenic bacteria Signup and view all the answers

Which material is NOT listed in the 'Materials' section for the experiment?

Nutrient Broth Signup and view all the answers

What is the purpose of using Tryptic Soy Broth (TSB) in the experiment?

To test for fermentative properties of microorganisms Signup and view all the answers

In the context of sterility testing, what role does Fluid Thioglycollate Medium (FTM) play?

Detecting anaerobic microorganisms Signup and view all the answers

What is the recommended incubation period for thorough evaluation of sterility in the tubes?

7 days Signup and view all the answers

What should be done if any signs of microbial growth are observed during the incubation period?

Record the observation Signup and view all the answers

What does the absence of growth in the test sample indicate?

Successful sterilization Signup and view all the answers

Which action confirms the viability of the media and the experimental setup during sterility testing?

Growth in the positive control Signup and view all the answers

What precaution is emphasized for handling microbial cultures during the sterility testing procedure?

Wearing appropriate PPE Signup and view all the answers

What should be done if turbidity is observed in one of the tubes during observation after incubation?

Discard the tube immediately Signup and view all the answers