Microbiology Lab Techniques

For suppurative lesions of deep skin and subcutaneous tissues, direct mounts are made by mixing a small portion of the sample in two or three drops of physiologic saline or KOH on a microscopic slide.

The circulatory system is always susceptible to microbial organisms.

Fungemia is a condition where bacteria are present in the blood.

Cutaneous samples for fungal infections are obtained by aspiration with a sterile needle and syringe.

The presence of bacteria or fungi in the blood is a common and usually harmless condition.

Gram staining is used to spread pus or exudate as thinly as possible on a clear glass slide.

Anaerobic bacteria can grow on solid media in room air with 0.04% carbon dioxide and 21% oxygen.

Culturing a sample of CSF is necessary to detect the presence of anaerobic bacteria.

PCR testing is used to detect the presence of fungi in the CSF.

Increased white blood cells in the CSF indicate inflammation.

Anaerobic infections are most commonly found in the respiratory tract.

Microscopic examination of CSF can reveal the presence of bacteria, viruses, or white blood cells.

Penicillin is used to identify anaerobic bacteria based on susceptibility.

Coy anaerobic chambers are used to isolate aerobic bacteria.

Robertson cooked meat media is used as a selective media for anaerobic bacteria.

Haemin and vitamin K are not typically added to brain heart infusion agar for anaerobic bacteria growth.

L-Cysteine is not used as a supplement in anaerobic bacteria growth media.

Sodium azide is not a selective agent used in anaerobic bacteria identification.