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Microbiology Lab Techniques Quiz

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Questions and Answers

What is the purpose of the streak plate technique?

To identify different types of bacteria in a sample

To observe bacterial growth under a microscope

To measure the rate of bacterial reproduction

To isolate organisms from a mixed population into a pure culture (correct)

In the transfer of broth to broth, why is the loop flamed after dipping into the new broth?

To prevent contamination of the new broth (correct)

To sterilize the loop

To ensure the loop is not too hot

To enhance the growth of bacteria in the new broth

Why is it important to flame the mouth of the culture tube before and after transferring the loop into the broth?

To enhance the visibility of bacteria

To provide heat for bacterial growth

To sterilize the mouth of the culture tube (correct)

To remove excess moisture from the tube

What is the purpose of flaming the loop before and after streaking a slant?

To sterilize the loop and prevent contamination Signup and view all the answers

Why is the inoculating loop flamed before and after use?

To sterilize the loop and kill any microorganisms Signup and view all the answers

What is the purpose of antibiotic susceptibility testing?

To select effective antimicrobial drugs Signup and view all the answers

When is susceptibility testing not usually indicated?

When the susceptibility reactions of a pathogen can be predicted Signup and view all the answers

How is testing individual pathogens against antibiotics typically done?

Disc diffusion, Tube dilution, E test Signup and view all the answers

What is the first step of the disc diffusion method for testing pathogens against antibiotics?

Inoculate the bacteria to be tested by homogenous streaking across the agar plate Signup and view all the answers

Why is isolating bacteria from a patient not enough for determining the proper therapy?

Because antibiotic susceptibility testing is required to select effective drugs Signup and view all the answers