10 Questions
What is the purpose of the streak plate technique?
To isolate organisms from a mixed population into a pure culture
In the transfer of broth to broth, why is the loop flamed after dipping into the new broth?
To prevent contamination of the new broth
Why is it important to flame the mouth of the culture tube before and after transferring the loop into the broth?
To sterilize the mouth of the culture tube
What is the purpose of flaming the loop before and after streaking a slant?
To sterilize the loop and prevent contamination
Why is the inoculating loop flamed before and after use?
To sterilize the loop and kill any microorganisms
What is the purpose of antibiotic susceptibility testing?
To select effective antimicrobial drugs
When is susceptibility testing not usually indicated?
When the susceptibility reactions of a pathogen can be predicted
How is testing individual pathogens against antibiotics typically done?
Disc diffusion, Tube dilution, E test
What is the first step of the disc diffusion method for testing pathogens against antibiotics?
Inoculate the bacteria to be tested by homogenous streaking across the agar plate
Why is isolating bacteria from a patient not enough for determining the proper therapy?
Because antibiotic susceptibility testing is required to select effective drugs
Test your knowledge of microbiology lab techniques with this quiz covering the proper way to hold an inoculating loop, flaming the loop, streaking a plate, and transferring cultures to tubes. This quiz will help you reinforce your understanding of essential microbiology lab skills.
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