Microbiology Lab Techniques Quiz
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Questions and Answers

What is the purpose of the streak plate technique?

  • To identify different types of bacteria in a sample
  • To observe bacterial growth under a microscope
  • To measure the rate of bacterial reproduction
  • To isolate organisms from a mixed population into a pure culture (correct)

In the transfer of broth to broth, why is the loop flamed after dipping into the new broth?

  • To prevent contamination of the new broth (correct)
  • To sterilize the loop
  • To ensure the loop is not too hot
  • To enhance the growth of bacteria in the new broth

Why is it important to flame the mouth of the culture tube before and after transferring the loop into the broth?

  • To enhance the visibility of bacteria
  • To provide heat for bacterial growth
  • To sterilize the mouth of the culture tube (correct)
  • To remove excess moisture from the tube

What is the purpose of flaming the loop before and after streaking a slant?

<p>To sterilize the loop and prevent contamination (C)</p> Signup and view all the answers

Why is the inoculating loop flamed before and after use?

<p>To sterilize the loop and kill any microorganisms (C)</p> Signup and view all the answers

What is the purpose of antibiotic susceptibility testing?

<p>To select effective antimicrobial drugs (B)</p> Signup and view all the answers

When is susceptibility testing not usually indicated?

<p>When the susceptibility reactions of a pathogen can be predicted (C)</p> Signup and view all the answers

How is testing individual pathogens against antibiotics typically done?

<p>Disc diffusion, Tube dilution, E test (B)</p> Signup and view all the answers

What is the first step of the disc diffusion method for testing pathogens against antibiotics?

<p>Inoculate the bacteria to be tested by homogenous streaking across the agar plate (A)</p> Signup and view all the answers

Why is isolating bacteria from a patient not enough for determining the proper therapy?

<p>Because antibiotic susceptibility testing is required to select effective drugs (C)</p> Signup and view all the answers

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