Microbiology Lab Techniques: Enriched Media and Phenol Red Test
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Questions and Answers

What is the purpose of using liquid media (broth) in bacterial culture?

  • To solidify the culture medium for better bacterial growth
  • To feed vitamins and amino acids to bacteria
  • To grow bacteria uniformly and produce turbidity (correct)
  • To classify bacteria based on their metabolic activities

What type of media is suitable when the number of bacteria in the inoculum is suspected to be low?

  • Solid media
  • Semi-solid media
  • Biphasic media
  • Liquid media (correct)

Which functional use of media helps in differentiating between different types of bacteria based on their characteristics?

  • Differential media (correct)
  • Enriched media
  • Transport media
  • Selective media

What type of media is used to support the growth of fungi in culture?

<p>Enriched media (D)</p> Signup and view all the answers

Which type of media is used to sustain the viability of microorganisms during transportation from one location to another?

<p>Transport media (D)</p> Signup and view all the answers

What is the primary function of using selective media in bacterial culture?

<p>To encourage the growth of specific microorganisms while inhibiting others (C)</p> Signup and view all the answers

Which type of culture media allows growth of one group of bacteria while inhibiting the growth of other groups?

<p>Selective media (A)</p> Signup and view all the answers

What is the purpose of adding specific substances to selective media?

<p>To allow growth of one group of bacteria and inhibit others (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of differential media?

<p>Inhibits the growth of undesired bacterial groups (C)</p> Signup and view all the answers

Which type of media is enriched with citrated blood to show types of haemolysis?

<p>CHOC (D)</p> Signup and view all the answers

What differentiates Beta haemolysis from Alpha haemolysis on blood agar?

<p>Partial lysis vs. complete lysis (A)</p> Signup and view all the answers

Which media is specifically used for research and where every component is well known?

<p>Davis &amp; Mingioli synthetic media (B)</p> Signup and view all the answers

Which culture plate is both selective and differential in nature?

<p>Mannitol Salt Agar (C)</p> Signup and view all the answers

Which of the following media supports the growth of fastidious bacteria through the addition of blood, serum, or extracts?

<p>Stuart medium (D)</p> Signup and view all the answers

What happens if mannitol is fermented in Mannitol Salt Agar?

<p>'Acidic products are formed (C)</p> Signup and view all the answers

Which type of media contains antibiotics like Gentamycin and Chloramphenicol to inhibit bacterial growth?

<p>BA (A)</p> Signup and view all the answers

In which media is eggs and glycerol added to support the growth of Mycobacterium tuberculosis?

<p>Lowenstein-Jensen medium (A)</p> Signup and view all the answers

Which media is a solution of buffers with carbohydrates, peptones, and nutrients but lacks growth factors?

<p>Amies medium (A)</p> Signup and view all the answers

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