Microbiology Lab Techniques: Enriched Media and Phenol Red Test

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What is the purpose of using liquid media (broth) in bacterial culture?

To solidify the culture medium for better bacterial growth

To feed vitamins and amino acids to bacteria

To grow bacteria uniformly and produce turbidity (correct)

To classify bacteria based on their metabolic activities

What type of media is suitable when the number of bacteria in the inoculum is suspected to be low?

Solid media

Semi-solid media

Biphasic media

Liquid media (correct)

Which functional use of media helps in differentiating between different types of bacteria based on their characteristics?

Differential media (correct)

Enriched media

Transport media

Selective media

What type of media is used to support the growth of fungi in culture?

Enriched media Signup and view all the answers

Which type of media is used to sustain the viability of microorganisms during transportation from one location to another?

Transport media Signup and view all the answers

What is the primary function of using selective media in bacterial culture?

To encourage the growth of specific microorganisms while inhibiting others Signup and view all the answers

Which type of culture media allows growth of one group of bacteria while inhibiting the growth of other groups?

Selective media Signup and view all the answers

What is the purpose of adding specific substances to selective media?

To allow growth of one group of bacteria and inhibit others Signup and view all the answers

Which of the following is NOT a characteristic of differential media?

Inhibits the growth of undesired bacterial groups Signup and view all the answers

Which type of media is enriched with citrated blood to show types of haemolysis?

CHOC Signup and view all the answers

What differentiates Beta haemolysis from Alpha haemolysis on blood agar?

Partial lysis vs. complete lysis Signup and view all the answers

Which media is specifically used for research and where every component is well known?

Davis & Mingioli synthetic media Signup and view all the answers

Which culture plate is both selective and differential in nature?

Mannitol Salt Agar Signup and view all the answers

Which of the following media supports the growth of fastidious bacteria through the addition of blood, serum, or extracts?

Stuart medium Signup and view all the answers

What happens if mannitol is fermented in Mannitol Salt Agar?

'Acidic products are formed Signup and view all the answers

Which type of media contains antibiotics like Gentamycin and Chloramphenicol to inhibit bacterial growth?

BA Signup and view all the answers

In which media is eggs and glycerol added to support the growth of Mycobacterium tuberculosis?

Lowenstein-Jensen medium Signup and view all the answers

Which media is a solution of buffers with carbohydrates, peptones, and nutrients but lacks growth factors?

Amies medium Signup and view all the answers