Introduction to Microbiology Quiz
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Questions and Answers

What is the main role of water in microbial growth?

  • It serves as a nutrient source for energy.
  • It is the primary medium for respiration.
  • It is necessary for maintaining cell structure and function. (correct)
  • It regulates the temperature of microbial environments.

    • Which temperature condition is likely to hinder bacterial growth?

  • Moderated room temperatures.
  • Extreme cold temperatures. (correct)
  • Temperature fluctuations.
  • Optimal temperatures for specific bacterial species.

    • Why is ultraviolet light used in sterilization?

  • It promotes the growth of beneficial microbes.
  • It enhances the enzymatic breakdown of nutrients.
  • It damages microbial DNA. (correct)
  • It increases the moisture content in the environment.

    • What effect does dehydration have on microbial growth?

    <p>Bacterial growth resumes with rehydration.</p> Signup and view all the answers

    What are the basic nutritional requirements for microbial growth?

    <p>Energy, nitrogen, carbon, water, and sulfur.</p> Signup and view all the answers

    Which microorganism is likely to survive longer outside the host in a dry environment?

    <p>Staphylococci.</p> Signup and view all the answers

    What is one effect of adding growth inhibitors to a culture medium?

    <p>It can retard microbial growth.</p> Signup and view all the answers

    What type of light is harmful to most parasitic microorganisms?

    <p>Ultraviolet light.</p> Signup and view all the answers

    Which of the following factors influences the establishment of the microbiome?

    <p>Mode of delivery</p> Signup and view all the answers

    What characterizes exotoxins compared to endotoxins?

    <p>They are generally more toxic than endotoxins.</p> Signup and view all the answers

    Which of the following is NOT a main division of micro-organisms?

    <p>Parasites</p> Signup and view all the answers

    Which statement best describes enzymes in the context of microbial activity?

    <p>They catalyze reactions without undergoing changes themselves.</p> Signup and view all the answers

    What is a distinguishing feature of eukaryotes compared to prokaryotes?

    <p>Have a defined nucleus</p> Signup and view all the answers

    What condition is typically NOT needed for microbial growth?

    <p>High temperature only</p> Signup and view all the answers

    What role do microbial metabolites play in dental health?

    <p>They lead to the production of organic acids.</p> Signup and view all the answers

    How does the presence of endotoxins typically influence the host?

    <p>They are less toxic than exotoxins.</p> Signup and view all the answers

    Which of the following terms accurately describes prokaryotic cells?

    <p>Lack a membrane-bound nucleus</p> Signup and view all the answers

    What is one way microbial enzymes contribute to bacterial invasiveness?

    <p>By degrading host defense responses.</p> Signup and view all the answers

    Micro-organisms are important in health and disease for which of the following reasons?

    <p>They are involved in infection control</p> Signup and view all the answers

    Which statement best reflects the characteristics of eukaryotic cells?

    <p>Larger and complex with membrane-bound organelles</p> Signup and view all the answers

    What is a common source of exotoxins?

    <p>Secreted proteins from bacteria.</p> Signup and view all the answers

    What is the primary impact of microbial metabolites in the context of dental disease?

    <p>They facilitate decay through acid production.</p> Signup and view all the answers

    In the context of micro-organisms, what does the term 'normal microflora' refer to?

    <p>Microbes that are part of a healthy human body</p> Signup and view all the answers

    Which of the following is an example of a prokaryote?

    <p>Bacteria</p> Signup and view all the answers

    What structure separates the stomach from the small intestine?

    <p>Pyloric sphincter</p> Signup and view all the answers

    Which of the following cells in the gastric glands is responsible for producing hydrochloric acid?

    <p>Parietal cells</p> Signup and view all the answers

    What is the primary function of the smooth muscle in the wall of the oesophagus?

    <p>Facilitation of peristalsis</p> Signup and view all the answers

    What adaptation allows the stomach lining to accommodate increased volume when eating?

    <p>Rugae</p> Signup and view all the answers

    Which hormone is associated with increasing gastric secretion and motility?

    <p>Gastrin</p> Signup and view all the answers

    What are the five main activities that occur in the digestive system?

    <p>Ingestion, Propulsion, Digestion, Absorption, Elimination</p> Signup and view all the answers

    Which of the following organs is NOT considered a component of the alimentary canal?

    <p>Liver</p> Signup and view all the answers

    Which function is NOT associated with gastric juice?

    <p>Neutralizing intestinal acids</p> Signup and view all the answers

    What is one of the main functions of the small intestine?

    <p>Chemical digestion and nutrient absorption</p> Signup and view all the answers

    Which tissue type is primarily involved in the lining of the digestive tract?

    <p>Epithelial tissue</p> Signup and view all the answers

    What enzyme is primarily responsible for digesting lipids in the small intestine?

    <p>Pancreatic lipase</p> Signup and view all the answers

    Which nutrient is primarily absorbed in the jejunum of the small intestine?

    <p>Amino acids</p> Signup and view all the answers

    Which of the following is NOT a process through which nutrient absorption occurs in the small intestine?

    <p>Mitosis</p> Signup and view all the answers

    Where are bile salts primarily absorbed in the digestive system?

    <p>Terminal ileum</p> Signup and view all the answers

    Which enzyme acts specifically at the beginning of protein digestion?

    <p>Pepsin</p> Signup and view all the answers

    What is the temporary food mixture called in the stomach?

    <p>Chyme</p> Signup and view all the answers

    What is the primary role of the pyloric sphincter in the stomach?

    <p>To regulate the emptying of chyme into the small intestine</p> Signup and view all the answers

    Which part of the small intestine is primarily responsible for the majority of digestion?

    <p>Duodenum</p> Signup and view all the answers

    What unique structures increase the surface area for absorption in the jejunum and ileum?

    <p>Villi</p> Signup and view all the answers

    Which ducts drain into the duodenum to assist in digestion?

    <p>Bile duct and pancreatic duct</p> Signup and view all the answers

    What kind of epithelium lines the villi where nutrient absorption occurs?

    <p>Simple columnar</p> Signup and view all the answers

    What type of substances do capillaries in the villi absorb?

    <p>Nutrients excluding fats</p> Signup and view all the answers

    How long is the duodenum, the first part of the small intestine?

    <p>25 cm</p> Signup and view all the answers

    What is the primary role of bile in the digestive process?

    <p>To emulsify fats and assist in their digestion</p> Signup and view all the answers

    Which substance is primarily stored and concentrated by the gall bladder?

    <p>Bile</p> Signup and view all the answers

    What triggers the gall bladder to release bile into the duodenum?

    <p>Cholecystokinin</p> Signup and view all the answers

    What is a significant consequence of gallstones?

    <p>Blockage of the hepatic or common bile ducts</p> Signup and view all the answers

    Which component of the pancreas plays a role in hormone regulation?

    <p>Endocrine part</p> Signup and view all the answers

    Which digestive enzymes are produced by the exocrine pancreas?

    <p>Lipase, amylase, and proteases</p> Signup and view all the answers

    What is the primary function of the small intestine besides digestion?

    <p>Absorption of nutrients</p> Signup and view all the answers

    What percentage of bile acids is typically reabsorbed in the ileum?

    <p>95%</p> Signup and view all the answers

    What mixture is formed in the mouth during mastication, making it ready for swallowing?

    <p>Bolus</p> Signup and view all the answers

    Which structure must be closed off to prevent the backflow of stomach contents during swallowing?

    <p>Oesophageal sphincter</p> Signup and view all the answers

    What is the role of chief cells found in the gastric glands?

    <p>Produce pepsin for protein digestion</p> Signup and view all the answers

    What structure separates the stomach from the small intestine?

    <p>Pyloric sphincter</p> Signup and view all the answers

    Which component of gastric juice is primarily responsible for denaturing proteins?

    <p>Hydrochloric acid</p> Signup and view all the answers

    Which enzyme initiates protein digestion in the stomach?

    <p>Pepsin</p> Signup and view all the answers

    What is the primary role of bile in digestion?

    <p>To emulsify fats</p> Signup and view all the answers

    In which part of the small intestine does the majority of nutrient absorption occur?

    <p>Jejunum</p> Signup and view all the answers

    Which of the following combinations correctly describes the end products of lipid digestion?

    <p>Fatty acids and glycerol</p> Signup and view all the answers

    How are amino acids and glucose transported after absorption?

    <p>Via the hepatic portal vein</p> Signup and view all the answers

    What is a major function of the liver?

    <p>Secretion of bile</p> Signup and view all the answers

    What role does bile play in digestion?

    <p>It emulsifies fats for enzymatic action</p> Signup and view all the answers

    What triggers the gall bladder to release bile into the duodenum?

    <p>The hormone cholecystokinin</p> Signup and view all the answers

    Which of the following best describes the gall bladder's function?

    <p>Stores and concentrates bile</p> Signup and view all the answers

    Which statement accurately reflects the composition of bile?

    <p>It includes bile salts and pigments</p> Signup and view all the answers

    What is one of the primary functions of the pancreas?

    <p>Regulates blood sugar levels</p> Signup and view all the answers

    Which of the following statements about bile salts is correct?

    <p>They emulsify fats for better digestion</p> Signup and view all the answers

    What is the main purpose of the capillaries in the small intestine?

    <p>To drain nutrient-rich lymph into the hepatic portal system</p> Signup and view all the answers

    What is the primary function of pancreatic amylase in digestion?

    <p>Convert carbohydrates into simple sugars</p> Signup and view all the answers

    Which component of pancreatic fluid helps neutralize stomach acidity?

    <p>Sodium bicarbonate</p> Signup and view all the answers

    What is mainly absorbed in the large intestine?

    <p>Vitamins produced by gut bacteria</p> Signup and view all the answers

    What primarily causes the production of gas in the large intestine?

    <p>Bacterial fermentation of undigested material</p> Signup and view all the answers

    What is one of the main functions of bile?

    <p>Emulsify fats</p> Signup and view all the answers

    Which of the following statements is true regarding the large intestine?

    <p>It stores solid waste until it is eliminated</p> Signup and view all the answers

    Which accessory organ secretes bile to assist in digestion?

    <p>Liver</p> Signup and view all the answers

    Which enzyme is primarily responsible for breaking down dietary lipids?

    <p>Pancreatic lipase</p> Signup and view all the answers

    What is the primary function of the alimentary tract?

    <p>To convert food into small nutritional molecules for absorption</p> Signup and view all the answers

    Which of the following processes is NOT one of the active processes of the alimentary tract?

    <p>Transport</p> Signup and view all the answers

    What role does mucus play in the digestive system?

    <p>It protects the lining of the digestive tract</p> Signup and view all the answers

    Which part of the digestive system consists of smooth muscle that provides peristalsis?

    <p>Muscularis</p> Signup and view all the answers

    Which of the following organs is classified as an accessory organ of the digestive system?

    <p>Pancreas</p> Signup and view all the answers

    What is the function of lysozyme in the digestive system?

    <p>To break down bacterial cell walls</p> Signup and view all the answers

    What structure lines the alimentary tract and is coated with mucus?

    <p>Mucosa</p> Signup and view all the answers

    Which digestive process involves moving food through the alimentary canal?

    <p>Propulsion</p> Signup and view all the answers

    Which of these components is NOT part of the alimentary canal?

    <p>Gall bladder</p> Signup and view all the answers

    What is the major role of the small intestine in digestion?

    <p>Majority of digestion and nutrient absorption</p> Signup and view all the answers

    Study Notes

    Microbiology Introduction

    • Microbiology studies organisms too small for the naked eye, typically between 0.1-0.2mm in diameter.
    • Key micro-organisms include bacteria, viruses, fungi, algae, and protozoa.

    Microbial Categorization

    • Division of micro-organisms:
      • Bacteria: Simple, unicellular organisms.
      • Viruses: Consist only of genetic material and a protein coat; require a host for replication.
      • Fungi: Can be single or multi-cellular; decomposers in ecosystems.
      • Protozoa: Unicellular, often motile and capable of complex behaviors.

    Eukaryotes vs Prokaryotes

    • Eukaryotes:
      • Complex, larger cells with a defined nucleus and membrane-bound organelles.
      • Can be unicellular or multicellular (e.g., fungi, protozoa).
    • Prokaryotes:
      • Smaller, simpler cells without a membrane-bound nucleus.
      • Always unicellular (e.g., bacteria).

    Importance of Microorganisms

    • Essential roles in health, disease prevention, clinical appearances, and host responses.
    • Affect local and global health impacts.

    Microbial Growth Conditions

    • Factors necessary for microbial growth:
      • Temperature
      • pH levels
      • Water availability
      • Light exposure
      • Nutritional needs

    Mechanisms of Disease by Micro-organisms

    • Micro-organisms can cause disease through:
      • Endotoxins: Produced within the bacterial cell and released upon cell death.
      • Exotoxins: Secreted by living bacteria; often more toxic.
      • Metabolites: Byproducts of metabolism impacting host health.
      • Enzymes: Stimulate infections and assist in the spread of microbes.

    Enzymes in Microbiology

    • Enzymes act as catalysts for biochemical reactions without changing themselves.
    • Support microbial invasiveness and degradation of host responses.

    Microbiome Diversity

    • Established at birth and influenced by factors like:
      • Mode of delivery
      • Diet
    • Diversity linked to health conditions, including obesity and diabetes.

    Environmental Influence on Microbial Growth

    • Temperature: Affects growth rates; optimal temperatures vary by species.
    • pH: Specific pH ranges favor certain bacterial species.
    • Moisture: Essential for growth; varies by species resilience to desiccation.
    • Light: Most microbes thrive in darkness; UV light can sterilize.
    • Nutritional Needs: Includes sources of energy, nitrogen, carbon, water, and sulfur; specific nutrient additions can enhance growth.

    Impact of Microbial Diseases

    • Associated with quality of life, emotional impact, transmission, mortality, and economic burdens related to treatment.

    Conclusion

    • Understanding microbiology is crucial for addressing health issues, enhancing disease prevention, and implementing effective treatment strategies.

    Digestive System Overview

    • The digestive system is responsible for breaking down food into smaller molecules that can be absorbed by the body.
    • The five key activities of the digestive system are: ingestion, propulsion, digestion, absorption, and elimination (defecation).

    The Alimentary Canal

    • The alimentary canal, also known as the gastrointestinal (GI) tract, is the continuous tube that runs from the mouth to the anus.
    • The organs of the alimentary canal include the mouth, pharynx, esophagus, stomach, small intestine, large intestine, rectum, and anus.
    • Accessory organs, including the salivary glands, liver, gallbladder, and pancreas, aid in digestion.

    Mouth

    • The mouth begins the digestion process.
    • The teeth mechanically break down food during mastication (chewing).
    • The salivary glands produce saliva, which contains enzymes that initiate the chemical breakdown of carbohydrates.
    • The tongue helps move food around the mouth and forms a bolus, which is then swallowed.

    Esophagus

    • The esophagus is a muscular tube, connecting the pharynx to the stomach.
    • Smooth muscle contractions called peristalsis propel food down the esophagus.

    Stomach

    • The stomach is a muscular, J-shaped organ located in the upper left abdomen.
    • It is divided into three regions: fundus, body, and pylorus.
    • Gastric juice, a mixture of hydrochloric acid, enzymes, and mucus, is secreted by the stomach lining.
    • Hydrochloric acid (HCl) denatures proteins and activates pepsinogen into pepsin.
    • Pepsin breaks down proteins into smaller polypeptides.
    • Mucus protects the stomach lining from the acidic environment.
    • The stomach churns food, mixing it with gastric juice to form chyme.
    • The pyloric sphincter regulates the flow of chyme from the stomach into the small intestine.

    Small Intestine

    • The small intestine is the longest part of the digestive tract.
    • It is divided into three sections: the duodenum, jejunum, and ileum.
    • The duodenum is the first part of the small intestine, and this is where the majority of chemical digestion takes place.
    • The jejunum and ileum are primarily responsible for nutrient absorption.
    • The small intestine has a large surface area for absorption due to folds, villi, and microvilli.
    • Villi contain capillaries and lacteals: capillaries absorb nutrients (except fats) into the bloodstream, and lacteals absorb fats into the lymphatic system.
    • The small intestine receives secretions from the pancreas and liver, which play a key role in digestion.

    Accessory Organs

    • Liver: The liver is the largest gland and the largest solid organ in the body. It produces bile, which is stored in the gallbladder. Bile emulsifies fats, breaking large fat globules into smaller ones.
    • Gallbladder: The gallbladder stores and concentrates bile. When fat enters the duodenum, the hormone cholecystokinin stimulates the gallbladder to release bile into the small intestine.
    • Pancreas: The pancreas serves both an exocrine and endocrine function. The exocrine function produces digestive enzymes: trypsin, chymotrypsin, carboxypeptidase, and pancreatic lipase, which are secreted into the duodenum. The endocrine function of the pancreas produces hormones such as insulin and glucagon, which regulate blood sugar levels.

    Large Intestine

    • The large intestine is shorter than the small intestine and does not play a primary role in digestion but absorbs water, salts, and some vitamins, preparing waste for elimination as feces.
    • It is divided into four sections: cecum, colon, rectum, and anus.

    Absorption

    • Most nutrients are absorbed in the small intestine, primarily in the jejunum.
    • Absorption can occur via simple diffusion, facilitated diffusion, and active transport.

    Digestion of Lipids

    • Bile salts emulsify fats, breaking them down into smaller droplets.
    • Pancreatic lipase splits these emulsified fats into fatty acids and glycerol.

    Digestion of Carbohydrates

    • Salivary amylase begins the digestion of carbohydrates in the mouth.
    • Pancreatic amylase continues carbohydrate digestion in the small intestine.

    Digestion of Proteins

    • Pepsin breaks down proteins into smaller polypeptides in the stomach.
    • Pancreatic enzymes like trypsin and chymotrypsin further break down polypeptides into smaller peptides in the small intestine.
    • Other enzymes like carboxypeptidase and peptidases complete the breakdown of peptides into amino acids.

    Summary of Digestion and Absorption Sites

    • Mouth: Starch digestion begins.
    • Stomach: Protein digestion begins, some water, lipids, caffeine, and alcohol are absorbed.
    • Small Intestine: Majority of digestion and absorption of carbohydrates, proteins, fats, water, vitamins, and minerals.
    • Large Intestine: Absorption of water, electrolytes, and some vitamins.

    Alimentary Tract

    • A tube extending from the mouth to the rectum.
    • Food is ground and mixed with digestive juices as it travels through the canal.

    Alimentary Tract Function

    • Converts food into small, nutritional molecules for absorption and distribution by circulation to all body tissues.
    • Excretes unused residue.
    • Active processes include:
      • Ingestion
      • Propulsion
      • Digestion
      • Absorption
      • Elimination (defaecation)
    • Part of the immune system:
      • Mucus (IgA)
      • Low pH
      • Lysozyme
      • Enzymes (saliva, bile)
    • Saliva in some animals acts as a poison to subdue prey.

    Components of the Digestive System

    • The mouth
    • The oesophagus
    • The stomach
    • The small and large intestines
    • The rectum

    Accessory Organs

    • Salivary glands
    • The Liver
    • The pancreas
    • The gall bladder

    General Structure of the Alimentary Tract

    • Walls of the digestive tract consist of four layers:
      • Adventitia/Serosa: Outer covering of connective tissue.
      • Muscularis: Smooth muscle providing contractions (peristalsis) to move food. Consists of two layers: outer longitudinal and inner circular.
      • Submucous: Loose connective tissue containing blood vessels, lymphatic vessels, nerves, and glands.
      • Mucosa: Lining epithelial layer coated with mucous.

    Stomach

    • Hollow muscular organ, J-shaped, located on the left side of the abdominal cavity (liver is on the right).
    • Recognizable regions: fundus, body, and pylorus.
    • Highly acidic environment.
    • Pyloric sphincter: Separates the stomach from the small intestine.
    • Oesophageal sphincter: Separates the stomach from the oesophagus.

    Structures of the Stomach

    • The lining of the stomach has numerous folds when empty called rugae, allowing for expansion when eating.
    • Numerous gastric glands secrete "gastric juice" into the stomach.
    • Common cells found in the glands:
      • Parietal cells: Produce HCl acid that denatures proteins.
      • Chief cells: Produce pepsin that begins protein digestion.
      • Mucus cells: Produce mucus protecting the stomach from hydrochloric action.

    Stomach Lining

    • Epithelium forms deep pits containing glands.
    • Glands secrete enzymes, hormones, and mucus.
    • Hormones like gastrin increase HCl secretion and stomach motility.
    • Stomach absorbs some water, lipids, caffeine, and alcohol.
    • The main function of the small intestine is absorption.

    Liver

    • The largest solid organ and largest gland in the body.
    • Multifunctional.
    • Secretes bile, which accumulates in the gall bladder between meals.
    • Capillaries of the small intestine drain fat and other nutrient-rich lymph into the liver via the hepatic portal system.

    Gall Bladder

    • Small, pear-shaped organ on the underside of the liver.
    • Stores and concentrates bile during the fasting state.
    • When fat enters the duodenum, mucosal cells release the peptide hormone cholecystokinin, stimulating the gall bladder to contract and discharge bile.
    • Bile is alkaline and makes food alkaline so that pancreatic enzymes can act on it.
    • Gallstones are usually cholesterol-based and may block the hepatic or common bile ducts, causing pain and jaundice.

    Bile

    • A watery greenish fluid produced by the liver and secreted via the hepatic duct and cystic duct to the gall bladder for storage.
    • Released on demand via the common bile duct to an opening near the pancreatic duct in the duodenum.
    • Contains bile salts/acids, bile pigments (mainly bilirubin), cholesterol, and phospholipids.
    • Bile salts and phospholipids emulsify fats, the rest are for excretion.
    • Large amounts of bile (acids) are secreted every day, but only a small amount is lost as 95% is reabsorbed in the ileum, returned to the liver, and reused.

    Pancreas

    • Both endocrine and exocrine gland.
    • The exocrine part produces many enzymes that enter the duodenum via the pancreatic duct.
    • The endocrine part produces insulin and glucagon.
    • The exocrine pancreas has two main digestive functions:
      • Secretes digestive enzymes in response to cholecystokinin to help with the digestion of fats, starches, and proteins.
      • Secretes an aqueous bicarbonate solution to help neutralize stomach acid entering the small intestine.

    Pancreatic Fluid

    • Contains:
      • Sodium bicarbonate to neutralize the acidity of contents arriving from the stomach (brings pH back up to 8).
      • Pancreatic amylase: Carbohydrates → simple sugars (along with maltase, sucrase, lactase).
      • Pancreatic lipase: Lipids → fatty acids & glycerol.
      • Trypsin & chymotrypsin: Protein → amino acids.
      • Nucleases: Hydrolyze ingested nucleic acids (RNA/DNA) into component nucleotides.

    Large Intestine (Colon)

    • No further breakdown of food occurs.
    • Absorption of remaining water from indigestible food matter.
    • Some electrolytes and indigestible food remain.
    • Stores and eliminates waste.
    • Absorbs vitamins produced by gut bacteria, especially vitamin K and certain B vitamins.

    Large Intestine Structure

    • Less muscular than the small intestine.
    • More goblet cells that secrete mucus for lubrication.
    • No villi.
    • Takes about 16 hours to complete remaining processes.
    • Gas production due to bacterial fermentation of undigested material.
    • Chyme mixed with mucus and bacteria (mostly commensals) → Faeces (given color by bile pigments).
    • Gut bacteria:
      • Synthesize vitamins.
      • Digest polysaccharides for which humans have no enzymes (10% of our calories).

    Rectum

    • Last 13 cm of the large intestine.
    • Stores solid waste until it leaves the body through the anus – external opening.
    • Peristaltic waves.
    • Sphincter relaxation (voluntary and involuntary).

    Digestion of Lipids

    • Bile produced by the liver is poured into the duodenum and brings about emulsification.
    • Pancreatic lipase splits lipid molecules into fatty acids and glycerol.

    Absorption

    • Stomach absorbs only a few substances.
    • Main absorption occurs in the small intestine.
    • Occurs by combination of simple diffusion, facilitated diffusion, and active transport.
    • Amino acids and glucose are transported directly to the liver via the hepatic portal vein.

    Absorption in the Small Intestine

    • Majority occurs in the jejunum.
    • Fatty acids and glycerol are absorbed by intestinal cells.
    • Iron absorbed in the duodenum.
    • Vitamin B12/bile salts absorbed in the terminal ileum.
    • Water and lipids absorbed throughout.

    Digestion and Absorption of Nutrients Summary

    • The table is not available for me to complete.

    Hormones and Enzymes

    • Amylase: Helps digestion of starch.
    • Pepsin: Helps digest protein.
    • Lipase: Helps digest lipids.

    Cholecystokinin

    • Stimulates the release of bile from the gall bladder.

    Bile Salts and Phospholipids

    • Emulsify fats, increasing their surface area for enzymatic digestion.

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