High Performance Liquid Chromatography (HPLC) - Dr. Thukaa Z. Abdul-Jalil Lecture 11&12
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Questions and Answers

What is the main function of High Performance Liquid Chromatography (HPLC)?

  • To identify and quantify compounds in liquid samples (correct)
  • To analyze compounds in gaseous samples
  • To determine the color of chemical compounds
  • To separate compounds in solid samples

    • What does the mobile phase in HPLC refer to?

  • The industrial chemicals used as samples
  • The compounds being analyzed
  • The solvent that moves through the system (correct)
  • The stationary phase used for separation

    • Under which elution mode does the mobile phase composition remain the same throughout the run?

  • Gradient elution
  • Reverse elution
  • Dynamic elution
  • Isocratic elution (correct)

    • What types of samples can High Performance Liquid Chromatography (HPLC) be applied to?

    <p>Pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic samples, and industrial chemicals</p> Signup and view all the answers

    What is the purpose of a high-pressure pump solvent manager in HPLC?

    <p>To generate and meter a specified flow rate of mobile phase</p> Signup and view all the answers

    What is the typical range of flow rates for analytical HPLC pumps?

    <p>0 to 10 ml/min</p> Signup and view all the answers

    What is the function of HPLC sample valves?

    <p>To control the flow of sample between the pump and the column</p> Signup and view all the answers

    Which type of detectors are most commonly employed in HPLC?

    <p>UV/Vis spectrophotometers, including diode array detectors</p> Signup and view all the answers

    What is a major difference between HPLC and GLC?

    <p>The separation in HPLC operates at ambient temperature, while GLC operates at high temperature.</p> Signup and view all the answers

    Why is the apparatus for HPLC more expensive than that for GLC?

    <p>HPLC requires a suitable pumping system and screw-jointed connections to withstand pressure.</p> Signup and view all the answers

    What types of compounds are mainly used in HPLC separation?

    <p>Non-volatile compounds</p> Signup and view all the answers

    What makes HPLC suitable for compounds that can be detected in the ultra-violet or visible regions of the spectrum?

    <p>The use of a porous polymer stationary phase</p> Signup and view all the answers

    What is the purpose of gradient elution in HPLC?

    <p>To change the mobile phase composition during the separation</p> Signup and view all the answers

    In HPLC, what does the reservoir (Solvent Delivery) hold?

    <p>The mobile phase</p> Signup and view all the answers

    What is the typical range of compounds that can be identified using HPLC?

    <p>Parts per trillion (ppt) to parts per quadrillion (ppq)</p> Signup and view all the answers

    Which elution mode in HPLC involves the mobile phase composition remaining constant throughout the run?

    <p>Isocratic elution</p> Signup and view all the answers

    What is the purpose of a high-pressure pump solvent manager in HPLC?

    <p>To regulate the flow rate of the mobile phase</p> Signup and view all the answers

    What is the typical range of flow rates for preparative HPLC pumps?

    <p>Above 100 ml/min</p> Signup and view all the answers

    What is the main function of HPLC sample valves?

    <p>To introduce the sample to the column under high pressure</p> Signup and view all the answers

    Which type of detectors are commonly employed in HPLC?

    <p>UV/Vis spectrophotometers</p> Signup and view all the answers

    What is a major difference between High Performance Liquid Chromatography (HPLC) and Gas Liquid Chromatography (GLC) based on the given text?

    <p>HPLC mainly works with non-volatile compounds, while GLC works with volatile compounds.</p> Signup and view all the answers

    Why is the apparatus for High Performance Liquid Chromatography (HPLC) more expensive than that for Gas Liquid Chromatography (GLC) according to the text?

    <p>HPLC operates at higher pressure, requiring screw-jointed connections, while GLC does not.</p> Signup and view all the answers

    Which types of compounds are mentioned in the text as being suitable for High Performance Liquid Chromatography (HPLC)?

    <p>Non-volatile terpenoids and phenolics</p> Signup and view all the answers

    What type of solvent mixture is used as the mobile phase in High Performance Liquid Chromatography (HPLC) according to the given text?

    <p>Variable gradient solvent mixture</p> Signup and view all the answers

    What is the typical range of sample volumes for preparative HPLC?

    <p>Greater than 10 ml</p> Signup and view all the answers

    What is the main function of HPLC sample valves?

    <p>To control the sample volume injected into the column</p> Signup and view all the answers

    Which of the following detectors may be used in High Performance Liquid Chromatography (HPLC)?

    <p>Mass spectrometers</p> Signup and view all the answers

    What is the elution strength of the mobile phase increased for as the separation proceeds in HPLC?

    <p>To reduce the retention of strongly retained sample components</p> Signup and view all the answers

    What is the purpose of gradient elution in HPLC?

    <p>To change the mobile phase composition during the separation</p> Signup and view all the answers

    Why is HPLC considered one of the most powerful tools in analytical chemistry?

    <p>It can identify compounds in trace concentrations as low as parts per trillion (ppt)</p> Signup and view all the answers

    What does the reservoir (Solvent Delivery) hold in HPLC?

    <p>Mobile phase</p> Signup and view all the answers

    What types of samples can HPLC be applied to?

    <p>Just about any sample such as pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic samples, and industrial chemicals</p> Signup and view all the answers

    What is the main reason for the higher cost of the apparatus for HPLC compared to GLC?

    <p>The need for a suitable pumping system and screw-jointed connections</p> Signup and view all the answers

    What does the mobile phase composition refer to in High Performance Liquid Chromatography (HPLC)?

    <p>The miscible solvent mixture that remains constant throughout the run</p> Signup and view all the answers

    Why is High Performance Liquid Chromatography (HPLC) mainly used for non-volatile compounds?

    <p>It operates at ambient temperature</p> Signup and view all the answers

    What makes High Performance Liquid Chromatography (HPLC) suitable for compounds detected in the ultra-violet or visible regions of the spectrum?

    <p>The ability to provide both quantitative and qualitative data in a single operation</p> Signup and view all the answers

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