High Performance Liquid Chromatography (HPLC) - Dr. Thukaa Z. Abdul-Jalil Lecture 11&12
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Questions and Answers

What is the main function of High Performance Liquid Chromatography (HPLC)?

  • To identify and quantify compounds in liquid samples (correct)
  • To analyze compounds in gaseous samples
  • To determine the color of chemical compounds
  • To separate compounds in solid samples

What does the mobile phase in HPLC refer to?

  • The industrial chemicals used as samples
  • The compounds being analyzed
  • The solvent that moves through the system (correct)
  • The stationary phase used for separation

Under which elution mode does the mobile phase composition remain the same throughout the run?

  • Gradient elution
  • Reverse elution
  • Dynamic elution
  • Isocratic elution (correct)

What types of samples can High Performance Liquid Chromatography (HPLC) be applied to?

<p>Pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic samples, and industrial chemicals (C)</p> Signup and view all the answers

What is the purpose of a high-pressure pump solvent manager in HPLC?

<p>To generate and meter a specified flow rate of mobile phase (A)</p> Signup and view all the answers

What is the typical range of flow rates for analytical HPLC pumps?

<p>0 to 10 ml/min (D)</p> Signup and view all the answers

What is the function of HPLC sample valves?

<p>To control the flow of sample between the pump and the column (D)</p> Signup and view all the answers

Which type of detectors are most commonly employed in HPLC?

<p>UV/Vis spectrophotometers, including diode array detectors (A)</p> Signup and view all the answers

What is a major difference between HPLC and GLC?

<p>The separation in HPLC operates at ambient temperature, while GLC operates at high temperature. (D)</p> Signup and view all the answers

Why is the apparatus for HPLC more expensive than that for GLC?

<p>HPLC requires a suitable pumping system and screw-jointed connections to withstand pressure. (B)</p> Signup and view all the answers

What types of compounds are mainly used in HPLC separation?

<p>Non-volatile compounds (A)</p> Signup and view all the answers

What makes HPLC suitable for compounds that can be detected in the ultra-violet or visible regions of the spectrum?

<p>The use of a porous polymer stationary phase (A)</p> Signup and view all the answers

What is the purpose of gradient elution in HPLC?

<p>To change the mobile phase composition during the separation (A)</p> Signup and view all the answers

In HPLC, what does the reservoir (Solvent Delivery) hold?

<p>The mobile phase (B)</p> Signup and view all the answers

What is the typical range of compounds that can be identified using HPLC?

<p>Parts per trillion (ppt) to parts per quadrillion (ppq) (B)</p> Signup and view all the answers

Which elution mode in HPLC involves the mobile phase composition remaining constant throughout the run?

<p>Isocratic elution (C)</p> Signup and view all the answers

What is the purpose of a high-pressure pump solvent manager in HPLC?

<p>To regulate the flow rate of the mobile phase (D)</p> Signup and view all the answers

What is the typical range of flow rates for preparative HPLC pumps?

<p>Above 100 ml/min (D)</p> Signup and view all the answers

What is the main function of HPLC sample valves?

<p>To introduce the sample to the column under high pressure (D)</p> Signup and view all the answers

Which type of detectors are commonly employed in HPLC?

<p>UV/Vis spectrophotometers (B)</p> Signup and view all the answers

What is a major difference between High Performance Liquid Chromatography (HPLC) and Gas Liquid Chromatography (GLC) based on the given text?

<p>HPLC mainly works with non-volatile compounds, while GLC works with volatile compounds. (B)</p> Signup and view all the answers

Why is the apparatus for High Performance Liquid Chromatography (HPLC) more expensive than that for Gas Liquid Chromatography (GLC) according to the text?

<p>HPLC operates at higher pressure, requiring screw-jointed connections, while GLC does not. (C)</p> Signup and view all the answers

Which types of compounds are mentioned in the text as being suitable for High Performance Liquid Chromatography (HPLC)?

<p>Non-volatile terpenoids and phenolics (B)</p> Signup and view all the answers

What type of solvent mixture is used as the mobile phase in High Performance Liquid Chromatography (HPLC) according to the given text?

<p>Variable gradient solvent mixture (B)</p> Signup and view all the answers

What is the typical range of sample volumes for preparative HPLC?

<p>Greater than 10 ml (C)</p> Signup and view all the answers

What is the main function of HPLC sample valves?

<p>To control the sample volume injected into the column (D)</p> Signup and view all the answers

Which of the following detectors may be used in High Performance Liquid Chromatography (HPLC)?

<p>Mass spectrometers (D)</p> Signup and view all the answers

What is the elution strength of the mobile phase increased for as the separation proceeds in HPLC?

<p>To reduce the retention of strongly retained sample components (C)</p> Signup and view all the answers

What is the purpose of gradient elution in HPLC?

<p>To change the mobile phase composition during the separation (A)</p> Signup and view all the answers

Why is HPLC considered one of the most powerful tools in analytical chemistry?

<p>It can identify compounds in trace concentrations as low as parts per trillion (ppt) (B)</p> Signup and view all the answers

What does the reservoir (Solvent Delivery) hold in HPLC?

<p>Mobile phase (D)</p> Signup and view all the answers

What types of samples can HPLC be applied to?

<p>Just about any sample such as pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic samples, and industrial chemicals (B)</p> Signup and view all the answers

What is the main reason for the higher cost of the apparatus for HPLC compared to GLC?

<p>The need for a suitable pumping system and screw-jointed connections (B)</p> Signup and view all the answers

What does the mobile phase composition refer to in High Performance Liquid Chromatography (HPLC)?

<p>The miscible solvent mixture that remains constant throughout the run (A)</p> Signup and view all the answers

Why is High Performance Liquid Chromatography (HPLC) mainly used for non-volatile compounds?

<p>It operates at ambient temperature (D)</p> Signup and view all the answers

What makes High Performance Liquid Chromatography (HPLC) suitable for compounds detected in the ultra-violet or visible regions of the spectrum?

<p>The ability to provide both quantitative and qualitative data in a single operation (B)</p> Signup and view all the answers

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