36 Questions
What is the main function of High Performance Liquid Chromatography (HPLC)?
To identify and quantify compounds in liquid samples
What does the mobile phase in HPLC refer to?
The solvent that moves through the system
Under which elution mode does the mobile phase composition remain the same throughout the run?
Isocratic elution
What types of samples can High Performance Liquid Chromatography (HPLC) be applied to?
Pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic samples, and industrial chemicals
What is the purpose of a high-pressure pump solvent manager in HPLC?
To generate and meter a specified flow rate of mobile phase
What is the typical range of flow rates for analytical HPLC pumps?
0 to 10 ml/min
What is the function of HPLC sample valves?
To control the flow of sample between the pump and the column
Which type of detectors are most commonly employed in HPLC?
UV/Vis spectrophotometers, including diode array detectors
What is a major difference between HPLC and GLC?
The separation in HPLC operates at ambient temperature, while GLC operates at high temperature.
Why is the apparatus for HPLC more expensive than that for GLC?
HPLC requires a suitable pumping system and screw-jointed connections to withstand pressure.
What types of compounds are mainly used in HPLC separation?
Non-volatile compounds
What makes HPLC suitable for compounds that can be detected in the ultra-violet or visible regions of the spectrum?
The use of a porous polymer stationary phase
What is the purpose of gradient elution in HPLC?
To change the mobile phase composition during the separation
In HPLC, what does the reservoir (Solvent Delivery) hold?
The mobile phase
What is the typical range of compounds that can be identified using HPLC?
Parts per trillion (ppt) to parts per quadrillion (ppq)
Which elution mode in HPLC involves the mobile phase composition remaining constant throughout the run?
Isocratic elution
What is the purpose of a high-pressure pump solvent manager in HPLC?
To regulate the flow rate of the mobile phase
What is the typical range of flow rates for preparative HPLC pumps?
Above 100 ml/min
What is the main function of HPLC sample valves?
To introduce the sample to the column under high pressure
Which type of detectors are commonly employed in HPLC?
UV/Vis spectrophotometers
What is a major difference between High Performance Liquid Chromatography (HPLC) and Gas Liquid Chromatography (GLC) based on the given text?
HPLC mainly works with non-volatile compounds, while GLC works with volatile compounds.
Why is the apparatus for High Performance Liquid Chromatography (HPLC) more expensive than that for Gas Liquid Chromatography (GLC) according to the text?
HPLC operates at higher pressure, requiring screw-jointed connections, while GLC does not.
Which types of compounds are mentioned in the text as being suitable for High Performance Liquid Chromatography (HPLC)?
Non-volatile terpenoids and phenolics
What type of solvent mixture is used as the mobile phase in High Performance Liquid Chromatography (HPLC) according to the given text?
Variable gradient solvent mixture
What is the typical range of sample volumes for preparative HPLC?
Greater than 10 ml
What is the main function of HPLC sample valves?
To control the sample volume injected into the column
Which of the following detectors may be used in High Performance Liquid Chromatography (HPLC)?
Mass spectrometers
What is the elution strength of the mobile phase increased for as the separation proceeds in HPLC?
To reduce the retention of strongly retained sample components
What is the purpose of gradient elution in HPLC?
To change the mobile phase composition during the separation
Why is HPLC considered one of the most powerful tools in analytical chemistry?
It can identify compounds in trace concentrations as low as parts per trillion (ppt)
What does the reservoir (Solvent Delivery) hold in HPLC?
Mobile phase
What types of samples can HPLC be applied to?
Just about any sample such as pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic samples, and industrial chemicals
What is the main reason for the higher cost of the apparatus for HPLC compared to GLC?
The need for a suitable pumping system and screw-jointed connections
What does the mobile phase composition refer to in High Performance Liquid Chromatography (HPLC)?
The miscible solvent mixture that remains constant throughout the run
Why is High Performance Liquid Chromatography (HPLC) mainly used for non-volatile compounds?
It operates at ambient temperature
What makes High Performance Liquid Chromatography (HPLC) suitable for compounds detected in the ultra-violet or visible regions of the spectrum?
The ability to provide both quantitative and qualitative data in a single operation
Explore the principles and applications of high performance liquid chromatography (HPLC) in analytical chemistry. Learn about its ability to separate, identify, and quantitate compounds in trace concentrations, making it a powerful tool for sample analysis.
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