HPLC Quiz

HPLC stands for high-performance liquid chromatography and is used to separate, identify, and quantify specific components in mixtures in analytical chemistry.

The mobile phase in HPLC refers to the mixtures of various solvents delivered by high pressure pumps through the system.

The stationary phase in HPLC is the solid particles, made of adsorbent material, packed in a cylinder through which the mobile phase flows, allowing for separation of components based on their interaction with the adsorbent material.

<p>HPLC separates components in mixtures based on their different interaction with the adsorbent material in the column, causing different migration rates and leading to their separation as they flow out of the column into a specific detector.</p> Signup and view all the answers

<p>The output of the detector in HPLC is a graph called Chromatogram, which shows the separated components.</p> Signup and view all the answers

<p>Non-aqueous titration is used to determine the concentration of analytes that are insoluble in water or react with water.</p> Signup and view all the answers

<p>Examples of analytes suitable for non-aqueous titration include organic compounds, certain pharmaceuticals, and substances that are sensitive to water.</p> Signup and view all the answers

<p>Advantages of non-aqueous titration over aqueous titration include the ability to work with non-water-soluble substances, higher sensitivity, and the avoidance of side reactions with water.</p> Signup and view all the answers

<p>Non-aqueous titration is used when the analyte or the titrant is insoluble or unstable in water. It is also employed for substances that react with water, such as certain organic compounds and weak acids or bases.</p> Signup and view all the answers

<p>Non-aqueous titration offers the advantage of increased solubility for a wider range of compounds, as well as the ability to titrate weak acids and bases without interference from water. It also allows for more precise determination of endpoints due to lower water content in the solvent.</p> Signup and view all the answers

<p>Common non-aqueous solvents used in non-aqueous titration include acetic acid, glacial acetic acid, perchloric acid, and formic acid. These solvents are chosen based on the specific requirements of the titration and the solubility of the substances involved.</p> Signup and view all the answers

<p>Non-aqueous titration is used for analyzing substances that are insoluble or unstable in water, such as certain organic compounds or non-aqueous solvents.</p> Signup and view all the answers

<p>Non-aqueous titration uses solvents other than water as the titrant and titrand, while aqueous titration uses water-based solutions.</p> Signup and view all the answers

<p>Some examples of non-aqueous titration applications include the analysis of oils, fats, waxes, and pharmaceuticals, as well as the determination of acid and base content in non-aqueous solvents.</p> Signup and view all the answers