12 Questions
What is the purpose of the high-pressure pump solvent manager in HPLC?
To generate and meter a specified flow rate of the mobile phase
What is the typical range for flow rates in analytical HPLC pumps?
0 to 10 ml/min
What type of detectors are commonly employed in HPLC?
UV/Vis spectrophotometers
What is the purpose of the sample valves in HPLC?
To tolerate pressures up to 10,000 psi
What is the main capability of high performance liquid chromatography (HPLC)?
Separating, identifying, and quantitating compounds in a sample
What is the purpose of the reservoir in HPLC instrumentation?
To hold the solvent used as the mobile phase
In what elution mode does the mobile phase composition remain the same throughout the run in HPLC?
Isocratic elution
When is gradient elution mode useful in HPLC?
For samples containing compounds with a wide range of chromatographic polarity
What is a major difference between HPLC and GLC?
HPLC operates at ambient temperature, while GLC operates at high temperatures.
Why is the apparatus for HPLC more expensive than GLC?
HPLC needs a suitable pumping system and operates under higher pressure.
For which classes of compounds is HPLC mainly used?
Non-volatile compounds
What is the role of the mobile phase in HPLC?
To change continuously in its proportions via a mixing chamber
Learn about the powerful tool of high performance liquid chromatography (HPLC) and its ability to separate, identify, and quantitate compounds in trace concentrations as low as parts per trillion (ppt). Discover its wide range of applications in analytical chemistry.
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