High Performance Liquid Chromatography (HPLC) Lecture 11&12
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Questions and Answers

What is the purpose of the high-pressure pump solvent manager in HPLC?

  • To generate and meter a specified flow rate of the eluent
  • To generate and meter a specified flow rate of the stationary phase
  • To generate and meter a specified flow rate of the mobile phase (correct)
  • To generate and meter a specified flow rate of the column

    • What is the typical range for flow rates in analytical HPLC pumps?

  • 10 to 20 ml/min
  • 15 to 25 ml/min
  • 0 to 10 ml/min (correct)
  • 5 to 15 ml/min

    • What type of detectors are commonly employed in HPLC?

  • Infrared spectrometers
  • Gas chromatographs
  • Nuclear magnetic resonance spectrometers
  • UV/Vis spectrophotometers (correct)

    • What is the purpose of the sample valves in HPLC?

    <p>To tolerate pressures up to 10,000 psi</p> Signup and view all the answers

    What is the main capability of high performance liquid chromatography (HPLC)?

    <p>Separating, identifying, and quantitating compounds in a sample</p> Signup and view all the answers

    What is the purpose of the reservoir in HPLC instrumentation?

    <p>To hold the solvent used as the mobile phase</p> Signup and view all the answers

    In what elution mode does the mobile phase composition remain the same throughout the run in HPLC?

    <p>Isocratic elution</p> Signup and view all the answers

    When is gradient elution mode useful in HPLC?

    <p>For samples containing compounds with a wide range of chromatographic polarity</p> Signup and view all the answers

    What is a major difference between HPLC and GLC?

    <p>HPLC operates at ambient temperature, while GLC operates at high temperatures.</p> Signup and view all the answers

    Why is the apparatus for HPLC more expensive than GLC?

    <p>HPLC needs a suitable pumping system and operates under higher pressure.</p> Signup and view all the answers

    For which classes of compounds is HPLC mainly used?

    <p>Non-volatile compounds</p> Signup and view all the answers

    What is the role of the mobile phase in HPLC?

    <p>To change continuously in its proportions via a mixing chamber</p> Signup and view all the answers

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