HPLC Fundamentals Quiz

Separation technique in which a liquid is passed through a column at high pressure. Used to separate, identify or quantify each component in a mixture. The basic principle of HPLC is that it separates a sample into its constituents based on the relative affinities of distinct molecules for the mobile phase and the stationary phase. The distribution of the analyte between a mobile phase (eluent) and a stationary phase (packing material of the column) is the basis for HPLC separation. The molecules are retarded while passing through the stationary phase. The duration a sample spends 'on-column' is determined by the unique intermolecular interactions between its molecules and the packing material, and hence the separation is achieved.

Used to quantify known materials. Identify unknown compounds within a sample. Elucidate the structure and chemical properties of molecules. The complete process involves.