10 Questions
What is fluorescence?
The emission of energy by an electron
What is necessary for emission to occur?
Absorption
What is a Jablonski diagram?
A diagram showing the energy levels of excited electrons
What is fluorescence resonance energy transfer (FRET)?
The transfer of energy between two fluorophores
What are the excitation and emission spectra generally like in fluorescence spectrophotometry?
Broad
What is the Stokes shift?
The difference between the positions of the band maxima of the absorption and emission spectra of the same electronic transition
When does FRET occur?
When the emission frequency of a fluorophore matches the excitation frequency of a nearby second fluorophore
What is the sensitivity of FRET to intermolecular distance?
Extremely sensitive
What can FRET be used for?
Measuring the relative dynamics between two molecules
What is the main cause of fluorescence?
The movement of an electron to a lower energy state
Study Notes
- The lecture is about fluorescence spectrophotometry.
- Fluorescence happens when an excited electron relaxes back to its ground state by emitting energy as photons.
- There cannot be emission without previous absorption.
- The lecture discusses the meaning of a Jablonski diagram.
- The lecture explains fluorescence resonance energy transfer (FRET) and its main properties.
- Excitation and emission spectra are generally broad.
- Stokes shift is the difference between positions of the band maxima of the absorption and emission spectra of the same electronic transition.
- FRET takes place when the emission frequency of a fluorophore matches the excitation frequency of a nearby second fluorophore.
- FRET is extremely sensitive to intermolecular distance.
- FRET can be used as an atomic "ruler" to measure the relative dynamics between two molecules.
Test your knowledge on fluorescence spectrophotometry with this quiz! Learn about the basics of fluorescence, absorption and emission spectra, Jablonski diagrams, and the properties of fluorescence resonance energy transfer (FRET). Challenge yourself with questions on the Stokes shift, intermolecular distance, and how FRET can be used as an atomic "ruler" to measure the relative dynamics between two molecules. Sharpen your understanding of this fascinating technique and boost your confidence in the subject!
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