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Fluorescence Spectrophotometry Quiz

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Questions and Answers

What is fluorescence?

The absorption of energy by an electron

The movement of an electron to a higher energy state

The emission of energy by an electron (correct)

The transfer of energy between two molecules

What is necessary for emission to occur?

Neither excitation nor absorption

Excitation

Absorption (correct)

Both excitation and absorption

What is a Jablonski diagram?

A type of fluorophore

A type of spectroscopy

A diagram showing the energy levels of ground state electrons

A diagram showing the energy levels of excited electrons (correct)

What is fluorescence resonance energy transfer (FRET)?

The transfer of energy between two fluorophores Signup and view all the answers

What are the excitation and emission spectra generally like in fluorescence spectrophotometry?

Broad Signup and view all the answers

What is the Stokes shift?

The difference between the positions of the band maxima of the absorption and emission spectra of the same electronic transition Signup and view all the answers

When does FRET occur?

When the emission frequency of a fluorophore matches the excitation frequency of a nearby second fluorophore Signup and view all the answers

What is the sensitivity of FRET to intermolecular distance?

Extremely sensitive Signup and view all the answers

What can FRET be used for?

Measuring the relative dynamics between two molecules Signup and view all the answers

What is the main cause of fluorescence?

The movement of an electron to a lower energy state Signup and view all the answers

Study Notes

The lecture is about fluorescence spectrophotometry.
Fluorescence happens when an excited electron relaxes back to its ground state by emitting energy as photons.
There cannot be emission without previous absorption.
The lecture discusses the meaning of a Jablonski diagram.
The lecture explains fluorescence resonance energy transfer (FRET) and its main properties.
Excitation and emission spectra are generally broad.
Stokes shift is the difference between positions of the band maxima of the absorption and emission spectra of the same electronic transition.
FRET takes place when the emission frequency of a fluorophore matches the excitation frequency of a nearby second fluorophore.
FRET is extremely sensitive to intermolecular distance.
FRET can be used as an atomic "ruler" to measure the relative dynamics between two molecules.

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Description

Test your knowledge on fluorescence spectrophotometry with this quiz! Learn about the basics of fluorescence, absorption and emission spectra, Jablonski diagrams, and the properties of fluorescence resonance energy transfer (FRET). Challenge yourself with questions on the Stokes shift, intermolecular distance, and how FRET can be used as an atomic "ruler" to measure the relative dynamics between two molecules. Sharpen your understanding of this fascinating technique and boost your confidence in the subject!