Podcast
Questions and Answers
What is the main purpose of FISH?
What is the main purpose of FISH?
- Detecting growth of cells in culture
- Detecting protein and RNA structures in situ
- Detecting chromosomal abnormalities in prenatal samples (correct)
- Detecting DNA structures in vitro
How is the probe specificity achieved in FISH?
How is the probe specificity achieved in FISH?
- By detecting chromosome structural abnormalities with telomeric probes
- By using fluorescently labeled DNA probe without specificity
- By increasing sensitivity using dual color probes
- By using probes designed for specific chromosomes or chromosomal regions (correct)
What does a deletion result in when detected through FISH?
What does a deletion result in when detected through FISH?
- More than two signals
- One signal (correct)
- Two signals per nucleus
- Increased sensitivity
What is the purpose of centromeric probes (CEPs) in FISH?
What is the purpose of centromeric probes (CEPs) in FISH?
What is the main purpose of overnight incubation for fresh interphase cells?
What is the main purpose of overnight incubation for fresh interphase cells?
What is a limitation of the Metaphase FISH technique?
What is a limitation of the Metaphase FISH technique?
What distinguishes Spectral Karyotyping from other techniques?
What distinguishes Spectral Karyotyping from other techniques?
How did the shift from nitrocellulose or nylon membranes to treated glass enhance array versatility?
How did the shift from nitrocellulose or nylon membranes to treated glass enhance array versatility?
What is a characteristic of microelectronic arrays?
What is a characteristic of microelectronic arrays?
What is the primary application of Expression Arrays?
What is the primary application of Expression Arrays?
Flashcards
FISH purpose
FISH purpose
Detecting abnormal chromosomes in prenatal samples
FISH probe specificity
FISH probe specificity
Probes target specific chromosomes or parts
FISH deletion signal
FISH deletion signal
One signal shows a missing part of a chromosome
Centromeric probes
Centromeric probes
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Overnight incubation
Overnight incubation
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Metaphase FISH limitation
Metaphase FISH limitation
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Spectral Karyotyping
Spectral Karyotyping
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Array versatility improvement
Array versatility improvement
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Microelectronic arrays
Microelectronic arrays
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Expression Arrays
Expression Arrays
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Prenatal FISH
Prenatal FISH
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Probe Design
Probe Design
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Macroarray to Microarray
Macroarray to Microarray
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Chromosomal Changes
Chromosomal Changes
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Genetic Marker Mapping
Genetic Marker Mapping
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Study Notes
FISH (Fluorescence In Situ Hybridization)
- The main purpose of FISH is to detect and localize specific DNA sequences in metaphase chromosomes, interphase nuclei, or whole cells.
- Probe specificity is achieved in FISH by designing probes that are complementary to specific DNA sequences, which then hybridize to the target sequence.
Detection of Deletions
- A deletion results in a lack of signal or a reduced signal intensity when detected through FISH.
Centromeric Probes (CEPs)
- The purpose of centromeric probes (CEPs) in FISH is to identify specific chromosomes and detect numerical aberrations.
Overnight Incubation
- The main purpose of overnight incubation for fresh interphase cells is to allow for efficient hybridization of probes to the target DNA sequences.
Metaphase FISH Technique
- A limitation of the Metaphase FISH technique is that it requires high-quality metaphase chromosome preparations.
Spectral Karyotyping
- Spectral Karyotyping is distinguished from other techniques by its ability to simultaneously identify all chromosomes in a single hybridization experiment.
Array Versatility
- The shift from nitrocellulose or nylon membranes to treated glass enhanced array versatility by allowing for the simultaneous analysis of thousands of gene sequences.
Microelectronic Arrays
- A characteristic of microelectronic arrays is that they are fabricated using photolithography and contain thousands of spots, each containing a specific DNA sequence.
Expression Arrays
- The primary application of Expression Arrays is to analyze the expression levels of thousands of genes in a single experiment, allowing for the identification of differentially expressed genes.
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Description
Test your knowledge of the principles and procedures of Fluorescence In Situ Hybridization (FISH), a technique used to detect protein, RNA, and DNA structures in situ for cytogenetic analysis. The quiz covers the process of probe hybridization, probe specificity, and visualization using fluorescent microscopy.
