Mastering UV Spectroscopy

The purpose of UV-VIS spectrophotometry is to measure the amount of ultraviolet (UV) and visible light absorbed by a sample.

The sample must absorb in the UV-Vis region and be a chromophore.

Absorption spectroscopy is complementary to fluorescence spectroscopy.

<p>The parameters of interest are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.</p> Signup and view all the answers

<p>UV-VIS spectrophotometry is widely used in chemistry, biochemistry, and other fields to identify and quantify compounds in various samples.</p> Signup and view all the answers

<p>To measure the amount of ultraviolet and visible light absorbed by a sample.</p> Signup and view all the answers

<p>Part of the ultraviolet and the full, adjacent visible regions.</p> Signup and view all the answers

<p>It is relatively inexpensive and easily implemented.</p> Signup and view all the answers

<p>The sample must absorb in the UV-Vis region, i.e., be a chromophore.</p> Signup and view all the answers

<p>Chemistry, biochemistry, and other fields, for compound identification and quantification.</p> Signup and view all the answers