10 Questions
What is the purpose of UV-VIS spectrophotometry?
The purpose of UV-VIS spectrophotometry is to measure the amount of ultraviolet (UV) and visible light absorbed by a sample.
What is the requirement for a sample to be suitable for UV-VIS spectrophotometry?
The sample must absorb in the UV-Vis region and be a chromophore.
How is absorption spectroscopy different from fluorescence spectroscopy?
Absorption spectroscopy is complementary to fluorescence spectroscopy.
What are the parameters of interest in UV-VIS spectrophotometry?
The parameters of interest are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.
In which fields is UV-VIS spectrophotometry widely used?
UV-VIS spectrophotometry is widely used in chemistry, biochemistry, and other fields to identify and quantify compounds in various samples.
What is the purpose of a UV-vis spectrophotometer?
To measure the amount of ultraviolet and visible light absorbed by a sample.
What is the range of the electromagnetic spectrum that UV-VIS spectrophotometry covers?
Part of the ultraviolet and the full, adjacent visible regions.
What is the main advantage of UV-VIS spectrophotometry?
It is relatively inexpensive and easily implemented.
What is the specific requirement for a sample to be suitable for UV-VIS spectrophotometry?
The sample must absorb in the UV-Vis region, i.e., be a chromophore.
What are some applications of UV-VIS spectrophotometry?
Chemistry, biochemistry, and other fields, for compound identification and quantification.
Test your knowledge of Ultra Violet (UV) spectroscopy and Ultra Violet–Visible (UV-VIS) spectrophotometry in this quiz. Learn about their principles, applications, and the requirements for implementing this widely used methodology.
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