Mastering UV Spectroscopy

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What is the purpose of UV-VIS spectrophotometry?

The purpose of UV-VIS spectrophotometry is to measure the amount of ultraviolet (UV) and visible light absorbed by a sample.

What is the requirement for a sample to be suitable for UV-VIS spectrophotometry?

The sample must absorb in the UV-Vis region and be a chromophore.

How is absorption spectroscopy different from fluorescence spectroscopy?

Absorption spectroscopy is complementary to fluorescence spectroscopy.

What are the parameters of interest in UV-VIS spectrophotometry?

The parameters of interest are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time. Signup and view all the answers

In which fields is UV-VIS spectrophotometry widely used?

UV-VIS spectrophotometry is widely used in chemistry, biochemistry, and other fields to identify and quantify compounds in various samples. Signup and view all the answers

What is the purpose of a UV-vis spectrophotometer?

To measure the amount of ultraviolet and visible light absorbed by a sample. Signup and view all the answers

What is the range of the electromagnetic spectrum that UV-VIS spectrophotometry covers?

Part of the ultraviolet and the full, adjacent visible regions. Signup and view all the answers

What is the main advantage of UV-VIS spectrophotometry?

It is relatively inexpensive and easily implemented. Signup and view all the answers

What is the specific requirement for a sample to be suitable for UV-VIS spectrophotometry?

The sample must absorb in the UV-Vis region, i.e., be a chromophore. Signup and view all the answers

What are some applications of UV-VIS spectrophotometry?

Chemistry, biochemistry, and other fields, for compound identification and quantification. Signup and view all the answers

Purpose of UV-VIS spectrophotometry

Measure the amount of UV and visible light absorbed by a sample.

Suitable sample for UV-VIS

A sample that absorbs UV/Vis light (chromophore).