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What are the two main groups of organisms classified under the Kingdom Procaryotae?
What are the two main groups of organisms classified under the Kingdom Procaryotae?
Bacteria and archaea.
Define taxonomy in the context of classification of living organisms.
Define taxonomy in the context of classification of living organisms.
Taxonomy is the science of classification of living organisms based on similarities and relationships.
What is the purpose of nomenclature in taxonomy?
What is the purpose of nomenclature in taxonomy?
Nomenclature assigns names to various taxa according to international rules.
Who developed the binomial system of nomenclature, and what are its two components?
Who developed the binomial system of nomenclature, and what are its two components?
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What are the three basic shapes of bacteria?
What are the three basic shapes of bacteria?
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What is binary fission in bacteria?
What is binary fission in bacteria?
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What does generation time refer to in bacterial reproduction?
What does generation time refer to in bacterial reproduction?
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What distinguishes protists from monerans?
What distinguishes protists from monerans?
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What is the significance of a flagella stain in identifying bacterial cells?
What is the significance of a flagella stain in identifying bacterial cells?
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Define chemolithotrophy and name the type of organisms that perform it.
Define chemolithotrophy and name the type of organisms that perform it.
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What advantage do phototrophic microorganisms have over chemotrophic organisms?
What advantage do phototrophic microorganisms have over chemotrophic organisms?
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Differentiate between heterotrophs and autotrophs.
Differentiate between heterotrophs and autotrophs.
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What is the largest phylum of Bacteria and what does it encompass?
What is the largest phylum of Bacteria and what does it encompass?
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Explain the term 'motile bacilli' and how they achieve motility.
Explain the term 'motile bacilli' and how they achieve motility.
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What is the role of pigments in phototrophic microorganisms?
What is the role of pigments in phototrophic microorganisms?
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Describe the relevance of pathogenic prokaryotes within the domain Bacteria.
Describe the relevance of pathogenic prokaryotes within the domain Bacteria.
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What term is used to describe organisms that thrive in extreme environments?
What term is used to describe organisms that thrive in extreme environments?
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What is the significance of the Domain Archaea in relation to extremophiles?
What is the significance of the Domain Archaea in relation to extremophiles?
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In which extreme environments can extremophiles commonly be found?
In which extreme environments can extremophiles commonly be found?
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What key characteristic distinguishes archaea from bacteria?
What key characteristic distinguishes archaea from bacteria?
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What do methanogens, a specific type of archaea, produce?
What do methanogens, a specific type of archaea, produce?
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What distinguishes obligate aerobes from microaerophilic aerobes?
What distinguishes obligate aerobes from microaerophilic aerobes?
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Give an example of a microaerophilic bacterium.
Give an example of a microaerophilic bacterium.
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What environment must obligate anaerobes be in to grow?
What environment must obligate anaerobes be in to grow?
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How do facultative anaerobes adapt to varying oxygen levels?
How do facultative anaerobes adapt to varying oxygen levels?
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What is an aerotolerant anaerobe's relationship with oxygen?
What is an aerotolerant anaerobe's relationship with oxygen?
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Identify a habitat where certain microorganisms can exist but humans cannot.
Identify a habitat where certain microorganisms can exist but humans cannot.
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Which family of bacteria includes many facultative anaerobes found in clinical specimens?
Which family of bacteria includes many facultative anaerobes found in clinical specimens?
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How do microaerophilic bacteria differ in oxygen requirements from obligate aerobes?
How do microaerophilic bacteria differ in oxygen requirements from obligate aerobes?
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What are the different arrangements of cocci bacteria?
What are the different arrangements of cocci bacteria?
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Describe the appearance of bacilli bacteria.
Describe the appearance of bacilli bacteria.
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What are spirochetes and how do they differ from bacilli?
What are spirochetes and how do they differ from bacilli?
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What is pleomorphism in bacteria?
What is pleomorphism in bacteria?
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Explain the significance of fixation in the staining process.
Explain the significance of fixation in the staining process.
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What is the difference between heat fixation and methanol fixation?
What is the difference between heat fixation and methanol fixation?
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What determines the color outcome of bacteria after a Gram stain procedure?
What determines the color outcome of bacteria after a Gram stain procedure?
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Who developed the Gram stain and what does it help observe?
Who developed the Gram stain and what does it help observe?
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What characteristic of Gram-positive bacteria makes them retain the crystal violet stain?
What characteristic of Gram-positive bacteria makes them retain the crystal violet stain?
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What role do specific stains play in observing bacterial morphology?
What role do specific stains play in observing bacterial morphology?
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How do Gram-negative bacteria differ in terms of their response to decolorization during the Gram staining procedure?
How do Gram-negative bacteria differ in terms of their response to decolorization during the Gram staining procedure?
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What are Gram-variable bacteria and provide an example?
What are Gram-variable bacteria and provide an example?
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What role does bacterial motility play and what structures are typically associated with it?
What role does bacterial motility play and what structures are typically associated with it?
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What is a common form of motility in bacteria that cannot swim?
What is a common form of motility in bacteria that cannot swim?
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How long has microbial diversity been evolving?
How long has microbial diversity been evolving?
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Name two examples of factors that contribute to microbial diversity.
Name two examples of factors that contribute to microbial diversity.
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Study Notes
Monerans and Protists
- Monerans include bacteria and archaea, classified in the Kingdom Procaryotae (or Bacteria)
- Protists include algae and protozoa and are in the Kingdom Protista
- Organisms in the kingdom Protista are called protists
Taxonomy
- Taxonomy, according to Bergey's Manual of Systematic Bacteriology, is the science of classifying living organisms
- It consists of classification, nomenclature, and identification
- Classification is the arrangement of organisms into taxonomic groups (taxa) based on similarities or relationships (e.g., kingdoms, domains, divisions, classes, orders)
- Nomenclature involves assigning names to taxa following international rules
- Identification determines if an isolate belongs to a known taxon or is a new species
Microbial Classification
- Microbial taxonomy uses a binomial system, developed by Carolus Linnaeus, giving each organism a genus and species name
- The first name is the genus
- The second name is the specific epithet
- Together, the genus and species name define the species
Cell Morphology
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Bacteria vary greatly in size (0.2 µm to 10.0µm) and shape
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Basic bacterial shapes include cocci (round or spherical), bacilli (rectangular or rod-shaped) and curved/spiral (spirilla).
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Some bacteria may lose their shape due to adverse growth conditions (e.g., antibiotics)
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Some revert to original shape when conditions improve
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Mycoplasma bacteria lack cell walls, appearing variable shapes microscopically
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Pleomorphic bacteria exist in various shapes, a characteristic called pleomorphism.
Staining Procedures
- Bacteria are smeared onto microscope slides, air-dried and fixed
- Fixation methods include heat fixation (passing through flame) or methanol fixation (flooding with absolute methanol for 30 seconds).
- Heat fixation may distort cell morphology and can be less effective than methanol fixation
- Specific stains are used to study bacterial size, shape, arrangement of cell wall components, capsules, flagella, and endospores
- Gram staining, developed by Hans Christian Gram, differentiates between Gram-positive and Gram-negative bacteria
- Gram-positive bacteria retain crystal violet-iodine complex, appearing blue-purple; Gram-negative do not and appear pink to red
- Some bacterial strains are Gram-variable meaning consistent blue/purple or pink/red color isn’t observed.
Bacterial Classification Based on Cell Shape
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Cocci:
- In pairs (diplococci)
- In chains (streptococci)
- In clusters (staphylococci)
- In packets of four (tetrads)
- In packets of eight (octads)
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Bacilli:
- In pairs (diplobacilli)
- In chains (streptobacilli)
- Some rods are short, resembling elongated cocci (coccobacilli)
- Curved and spiral-shaped bacilli are classified as spirochetes.
Motility
- Bacteria classified as “motile” can “swim” due to their ability to move by means of flagella
- Bacteria lacking flagella are termed nonmotile
- Other methods of motility include gliding along a secreted slime layer.
Metabolic Diversity
- All cells require energy-consuming life processes, which is done via various metabolic strategies
- Energy can be captured from organic compounds (heterotrophs) or from carbon dioxide (autotrophs)
-Chemolithotrophs obtain energy from inorganic chemicals
- Phototrophs capture energy from light
- Bacteria can be classified based on their metabolic requirements
Atmospheric Requirements
- Five major groups for oxygen requirements include obligate aerobes, microaerophiles, facultative anaerobes, aerotolerant anaerobes, and obligate anaerobes,
Habitats and Extreme Environments
- Extremophiles are microorganisms living in extreme environments (e.g., hot springs, acidic conditions, high salinity, deep ocean pressures).
- Bacteria and archaea have demonstrated tremendous adaptability and range of environments.
- Table 2.1 shows classification by extreme
Archaea
- Archaea are prokaryotes that were discovered in 1977.
- They were initially believed to have evolved prior to bacteria
- Later, they were found to be closer to eukaryotic organisms
- They are often extremophiles, living in extreme environments.
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Description
This quiz covers key concepts related to the classification of prokaryotes, particularly within the Kingdom Procaryotae. It explores topics such as taxonomy, nomenclature, bacterial shapes, and metabolic processes. Test your understanding of microbial characteristics and their ecological roles.