Biology Chapter on Prokaryotes and Taxonomy
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Questions and Answers

What are the two main groups of organisms classified under the Kingdom Procaryotae?

Bacteria and archaea.

Define taxonomy in the context of classification of living organisms.

Taxonomy is the science of classification of living organisms based on similarities and relationships.

What is the purpose of nomenclature in taxonomy?

Nomenclature assigns names to various taxa according to international rules.

Who developed the binomial system of nomenclature, and what are its two components?

<p>Carolus Linnaeus developed the binomial system, which consists of the genus and the specific epithet.</p> Signup and view all the answers

What are the three basic shapes of bacteria?

<p>Cocci (spherical), bacilli (rod-shaped), and spirilla (curved or spiral-shaped).</p> Signup and view all the answers

What is binary fission in bacteria?

<p>Binary fission is the process by which a bacterial cell splits into two cells.</p> Signup and view all the answers

What does generation time refer to in bacterial reproduction?

<p>Generation time refers to the time it takes for one bacterial cell to divide into two cells.</p> Signup and view all the answers

What distinguishes protists from monerans?

<p>Protists, classified in the Kingdom Protista, include algae and protozoa, while monerans refer specifically to bacteria and archaea.</p> Signup and view all the answers

What is the significance of a flagella stain in identifying bacterial cells?

<p>A flagella stain helps demonstrate the presence, number, and location of flagella on bacterial cells.</p> Signup and view all the answers

Define chemolithotrophy and name the type of organisms that perform it.

<p>Chemolithotrophy is a metabolic process where organisms obtain energy from inorganic compounds, and those organisms are called chemolithotrophs.</p> Signup and view all the answers

What advantage do phototrophic microorganisms have over chemotrophic organisms?

<p>Phototrophic microorganisms can convert light energy into chemical energy, avoiding competition for chemical energy sources.</p> Signup and view all the answers

Differentiate between heterotrophs and autotrophs.

<p>Heterotrophs require organic compounds for carbon, while autotrophs use carbon dioxide (CO2) as their carbon source.</p> Signup and view all the answers

What is the largest phylum of Bacteria and what does it encompass?

<p>The largest phylum of Bacteria is Proteobacteria, which includes a diverse range of prokaryotic species.</p> Signup and view all the answers

Explain the term 'motile bacilli' and how they achieve motility.

<p>Motile bacilli are bacteria that can move using flagella as their means of locomotion.</p> Signup and view all the answers

What is the role of pigments in phototrophic microorganisms?

<p>Pigments in phototrophic microorganisms allow them to capture light energy for converting into chemical energy.</p> Signup and view all the answers

Describe the relevance of pathogenic prokaryotes within the domain Bacteria.

<p>Pathogenic prokaryotes within the domain Bacteria are responsible for causing diseases in various hosts.</p> Signup and view all the answers

What term is used to describe organisms that thrive in extreme environments?

<p>Extremophiles</p> Signup and view all the answers

What is the significance of the Domain Archaea in relation to extremophiles?

<p>The Domain Archaea includes many extremophiles that live in extreme environments such as very high or low pH levels.</p> Signup and view all the answers

In which extreme environments can extremophiles commonly be found?

<p>Extremophiles can be found in volcanic hot springs, polar ice, extremely salty water, and deep-sea hydrothermal vents.</p> Signup and view all the answers

What key characteristic distinguishes archaea from bacteria?

<p>Archaea are genetically more closely related to eukaryotes than to bacteria.</p> Signup and view all the answers

What do methanogens, a specific type of archaea, produce?

<p>Methanogens produce methane.</p> Signup and view all the answers

What distinguishes obligate aerobes from microaerophilic aerobes?

<p>Obligate aerobes require oxygen concentrations similar to that of room air, while microaerophilic aerobes need lower concentrations of oxygen for growth.</p> Signup and view all the answers

Give an example of a microaerophilic bacterium.

<p>Neisseria gonorrhoeae is an example of a microaerophilic bacterium.</p> Signup and view all the answers

What environment must obligate anaerobes be in to grow?

<p>Obligate anaerobes must grow in an anaerobic environment, which contains no oxygen.</p> Signup and view all the answers

How do facultative anaerobes adapt to varying oxygen levels?

<p>Facultative anaerobes can survive in both the presence and absence of oxygen, adapting between 0% to 21% O2.</p> Signup and view all the answers

What is an aerotolerant anaerobe's relationship with oxygen?

<p>Aerotolerant anaerobes do not require oxygen for growth but can survive in its presence.</p> Signup and view all the answers

Identify a habitat where certain microorganisms can exist but humans cannot.

<p>Microorganisms can thrive in extreme environments like boiling hot springs or highly acidic lakes.</p> Signup and view all the answers

Which family of bacteria includes many facultative anaerobes found in clinical specimens?

<p>The family Enterobacteriaceae includes many facultative anaerobes commonly isolated from clinical specimens.</p> Signup and view all the answers

How do microaerophilic bacteria differ in oxygen requirements from obligate aerobes?

<p>Microaerophilic bacteria require lower oxygen levels than obligate aerobes, who need higher oxygen concentrations.</p> Signup and view all the answers

What are the different arrangements of cocci bacteria?

<p>Cocci bacteria can be arranged in pairs (diplococci), chains (streptococci), clusters (staphylococci), packets of four (tetrad), and packets of eight (octads).</p> Signup and view all the answers

Describe the appearance of bacilli bacteria.

<p>Bacilli bacteria appear as rod-shaped structures, and they can also exist in pairs (diplobacilli) or chains (streptobacilli).</p> Signup and view all the answers

What are spirochetes and how do they differ from bacilli?

<p>Spirochetes are spiral-shaped bacteria, which differ from bacilli that are typically rod-shaped. Spirochetes have a unique morphology that sets them apart from traditional bacilli.</p> Signup and view all the answers

What is pleomorphism in bacteria?

<p>Pleomorphism is the ability of bacteria to exist in a variety of shapes, and it is particularly seen in bacteria in the genus Mycoplasma. These bacteria lack cell walls, leading to their diverse morphologies.</p> Signup and view all the answers

Explain the significance of fixation in the staining process.

<p>Fixation is crucial for preserving bacterial morphology during staining and can be accomplished through methods such as heat fixation or methanol fixation. Proper fixation prevents distortion of the cell's appearance.</p> Signup and view all the answers

What is the difference between heat fixation and methanol fixation?

<p>Heat fixation involves passing the smear through a flame, which can potentially distort cell morphology, while methanol fixation involves flooding the smear with methanol, providing a more reliable fixation technique.</p> Signup and view all the answers

What determines the color outcome of bacteria after a Gram stain procedure?

<p>The color depends on the chemical composition of the bacterial cell wall.</p> Signup and view all the answers

Who developed the Gram stain and what does it help observe?

<p>Dr. Hans Christian Gram developed the Gram stain in 1883, which helps observe bacterial cell morphology, such as size, shape, arrangement, and cell wall composition.</p> Signup and view all the answers

What characteristic of Gram-positive bacteria makes them retain the crystal violet stain?

<p>They have a thick layer of peptidoglycan in their cell walls.</p> Signup and view all the answers

What role do specific stains play in observing bacterial morphology?

<p>Specific stains are used to enhance the visual characteristics of bacteria, allowing for the observation of details such as shape, size, cell wall structure, and additional features like capsules or flagella.</p> Signup and view all the answers

How do Gram-negative bacteria differ in terms of their response to decolorization during the Gram staining procedure?

<p>Gram-negative bacteria have a thin layer of peptidoglycan, allowing the crystal violet–iodine complex to be easily removed.</p> Signup and view all the answers

What are Gram-variable bacteria and provide an example?

<p>Gram-variable bacteria do not consistently display either blue to purple or pink to red after staining; an example is Mycobacterium tuberculosis.</p> Signup and view all the answers

What role does bacterial motility play and what structures are typically associated with it?

<p>Bacterial motility is crucial for movement and is often associated with flagella or axial filaments.</p> Signup and view all the answers

What is a common form of motility in bacteria that cannot swim?

<p>Bacteria that cannot swim exhibit glucose or alternative forms of motility.</p> Signup and view all the answers

How long has microbial diversity been evolving?

<p>Microbial diversity has been evolving for nearly 4 billion years.</p> Signup and view all the answers

Name two examples of factors that contribute to microbial diversity.

<p>Cell size and morphology (shape) are two factors contributing to microbial diversity.</p> Signup and view all the answers

Study Notes

Monerans and Protists

  • Monerans include bacteria and archaea, classified in the Kingdom Procaryotae (or Bacteria)
  • Protists include algae and protozoa and are in the Kingdom Protista
  • Organisms in the kingdom Protista are called protists

Taxonomy

  • Taxonomy, according to Bergey's Manual of Systematic Bacteriology, is the science of classifying living organisms
  • It consists of classification, nomenclature, and identification
  • Classification is the arrangement of organisms into taxonomic groups (taxa) based on similarities or relationships (e.g., kingdoms, domains, divisions, classes, orders)
  • Nomenclature involves assigning names to taxa following international rules
  • Identification determines if an isolate belongs to a known taxon or is a new species

Microbial Classification

  • Microbial taxonomy uses a binomial system, developed by Carolus Linnaeus, giving each organism a genus and species name
  • The first name is the genus
  • The second name is the specific epithet
  • Together, the genus and species name define the species

Cell Morphology

  • Bacteria vary greatly in size (0.2 µm to 10.0µm) and shape

  • Basic bacterial shapes include cocci (round or spherical), bacilli (rectangular or rod-shaped) and curved/spiral (spirilla).

  • Some bacteria may lose their shape due to adverse growth conditions (e.g., antibiotics)

  • Some revert to original shape when conditions improve

  • Mycoplasma bacteria lack cell walls, appearing variable shapes microscopically

  • Pleomorphic bacteria exist in various shapes, a characteristic called pleomorphism.

Staining Procedures

  • Bacteria are smeared onto microscope slides, air-dried and fixed
  • Fixation methods include heat fixation (passing through flame) or methanol fixation (flooding with absolute methanol for 30 seconds).
  • Heat fixation may distort cell morphology and can be less effective than methanol fixation
  • Specific stains are used to study bacterial size, shape, arrangement of cell wall components, capsules, flagella, and endospores
  • Gram staining, developed by Hans Christian Gram, differentiates between Gram-positive and Gram-negative bacteria
  • Gram-positive bacteria retain crystal violet-iodine complex, appearing blue-purple; Gram-negative do not and appear pink to red
  • Some bacterial strains are Gram-variable meaning consistent blue/purple or pink/red color isn’t observed.

Bacterial Classification Based on Cell Shape

  • Cocci:
    • In pairs (diplococci)
    • In chains (streptococci)
    • In clusters (staphylococci)
    • In packets of four (tetrads)
    • In packets of eight (octads)
  • Bacilli:
    • In pairs (diplobacilli)
    • In chains (streptobacilli)
    • Some rods are short, resembling elongated cocci (coccobacilli)
    • Curved and spiral-shaped bacilli are classified as spirochetes.

Motility

  • Bacteria classified as “motile” can “swim” due to their ability to move by means of flagella
  • Bacteria lacking flagella are termed nonmotile
  • Other methods of motility include gliding along a secreted slime layer.

Metabolic Diversity

  • All cells require energy-consuming life processes, which is done via various metabolic strategies
  • Energy can be captured from organic compounds (heterotrophs) or from carbon dioxide (autotrophs) -Chemolithotrophs obtain energy from inorganic chemicals
    • Phototrophs capture energy from light
  • Bacteria can be classified based on their metabolic requirements

Atmospheric Requirements

  • Five major groups for oxygen requirements include obligate aerobes, microaerophiles, facultative anaerobes, aerotolerant anaerobes, and obligate anaerobes,

Habitats and Extreme Environments

  • Extremophiles are microorganisms living in extreme environments (e.g., hot springs, acidic conditions, high salinity, deep ocean pressures).
  • Bacteria and archaea have demonstrated tremendous adaptability and range of environments.
  • Table 2.1 shows classification by extreme

Archaea

  • Archaea are prokaryotes that were discovered in 1977.
  • They were initially believed to have evolved prior to bacteria
  • Later, they were found to be closer to eukaryotic organisms
  • They are often extremophiles, living in extreme environments.

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Description

This quiz covers key concepts related to the classification of prokaryotes, particularly within the Kingdom Procaryotae. It explores topics such as taxonomy, nomenclature, bacterial shapes, and metabolic processes. Test your understanding of microbial characteristics and their ecological roles.

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