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Questions and Answers
What is the primary role of exoenzymes secreted by bacteria?
What is the primary role of exoenzymes secreted by bacteria?
What indicates that starch has been hydrolyzed in an experiment?
What indicates that starch has been hydrolyzed in an experiment?
What is the primary function of catalase in aerobic respiration?
What is the primary function of catalase in aerobic respiration?
Which of the following is a common byproduct of carbohydrate fermentation?
Which of the following is a common byproduct of carbohydrate fermentation?
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What does a red solution indicate in a fermentation experiment using phenol red?
What does a red solution indicate in a fermentation experiment using phenol red?
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Study Notes
Enzymes
- Enzymes are proteins composed of monomers.
- Exoenzymes are secreted by bacteria to break down large nutrients into smaller ones externally.
- Endoenzymes break down nutrients to release energy inside the bacteria.
Starch Hydrolysis
- Starch is a polysaccharide made of glucose.
- The enzyme diastase (amylase+maltase) breaks down starch.
- The substrate is starch, and the product is glucose.
- Iodine reacts with starch, forming a dark blue iodine-starch complex.
- A lack of dark blue color indicates hydrolysis of starch.
Catalase Activity
- Catalase breaks down hydrogen peroxide (H₂O₂) into water (H₂O) and oxygen (O₂) during aerobic respiration.
- Bubbles indicate catalase activity.
Fermentation of Carbohydrates
- Fermentation is an anaerobic process where carbohydrates are broken down for energy.
- Common fermentation byproducts include acids and/or gases.
- The media used often contains a pH indicator (like phenol red) and a carbohydrate.
- A Durham tube detects gas production.
- If no fermentation happens the solution remains red and does not turn yellow.
Biochemical Test Lab
- Tests can determine if a microbial cell wall is present,
- Can test for catalase activity using hydrogen peroxide and identifying oxygen production
- Tests for microbial enzymes can check for diastase, or catalase production.
- Gram reaction can be tested.
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Description
This quiz covers the essential concepts of enzymes, starch hydrolysis, catalase activity, and fermentation processes. Test your understanding of how these biological catalysts function and their importance in breaking down nutrients. Questions will explore the roles of exoenzymes, endoenzymes, and the biochemical reactions involved.