Biology Chapter: Enzymes and Fermentation
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Questions and Answers

What is the primary role of exoenzymes secreted by bacteria?

  • To form a protective barrier against external toxins
  • To break down large nutrients into smaller ones in the environment (correct)
  • To convert glucose into starch
  • To produce energy for the bacteria internally
  • What indicates that starch has been hydrolyzed in an experiment?

  • The formation of a dark blue color with iodine
  • The absence of a dark blue color after iodine is added (correct)
  • Bubbles forming during the reaction
  • The solution turning red in color
  • What is the primary function of catalase in aerobic respiration?

  • To convert glucose into energy
  • To break down hydrogen peroxide into harmless products (correct)
  • To synthesize energy from starch
  • To accelerate the fermentation of carbohydrates
  • Which of the following is a common byproduct of carbohydrate fermentation?

    <p>Acids and gases</p> Signup and view all the answers

    What does a red solution indicate in a fermentation experiment using phenol red?

    <p>No fermentation has happened</p> Signup and view all the answers

    Study Notes

    Enzymes

    • Enzymes are proteins composed of monomers.
    • Exoenzymes are secreted by bacteria to break down large nutrients into smaller ones externally.
    • Endoenzymes break down nutrients to release energy inside the bacteria.

    Starch Hydrolysis

    • Starch is a polysaccharide made of glucose.
    • The enzyme diastase (amylase+maltase) breaks down starch.
    • The substrate is starch, and the product is glucose.
    • Iodine reacts with starch, forming a dark blue iodine-starch complex.
    • A lack of dark blue color indicates hydrolysis of starch.

    Catalase Activity

    • Catalase breaks down hydrogen peroxide (H₂O₂) into water (H₂O) and oxygen (O₂) during aerobic respiration.
    • Bubbles indicate catalase activity.

    Fermentation of Carbohydrates

    • Fermentation is an anaerobic process where carbohydrates are broken down for energy.
    • Common fermentation byproducts include acids and/or gases.
    • The media used often contains a pH indicator (like phenol red) and a carbohydrate.
    • A Durham tube detects gas production.
    • If no fermentation happens the solution remains red and does not turn yellow.

    Biochemical Test Lab

    • Tests can determine if a microbial cell wall is present,
    • Can test for catalase activity using hydrogen peroxide and identifying oxygen production
    • Tests for microbial enzymes can check for diastase, or catalase production.
    • Gram reaction can be tested.

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    Description

    This quiz covers the essential concepts of enzymes, starch hydrolysis, catalase activity, and fermentation processes. Test your understanding of how these biological catalysts function and their importance in breaking down nutrients. Questions will explore the roles of exoenzymes, endoenzymes, and the biochemical reactions involved.

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