Biochemistry Lab Report (105 Lab 8)
Document Details
Uploaded by MagnificentEducation8280
Tags
Summary
The document is a report for a biochemistry lab, specifically focusing on enzyme activity, starch hydrolysis, and fermentation. The text outlines the different processes and tests performed, as well as their expected results.
Full Transcript
Enzymesareproteinsmade ofmonomers Exo enzymes secreted bybacteriaintothesurroundingenvironmenttobreakdownlargenutrientsintosmallerone downofnutrientto encymes break Endo yieldenergyinsi...
Enzymesareproteinsmade ofmonomers Exo enzymes secreted bybacteriaintothesurroundingenvironmenttobreakdownlargenutrientsintosmallerone downofnutrientto encymes break Endo yieldenergyinsidethebacteria starch Hydrolysis starchisapolysaccharidethatismadeof glucose Enzymediastaseamylasemaltase substratestarch product glucose Toseearesultiodineisaddedtostarchtoformiodinestarchcomplexformingadarkbluecolor thehydrolyzedzonewillshownoreaction nodarkbluecolorwhichmeansthestarchinthisareahasbeenhydrolyzed Catalase Activity break Catalase downhydrogenperoxide stoodintoHeoand orduringaerobicrespiration Aformationofbubblesindicatesthecatalaseactivity Fermentation of Carbohydrates Fermentation anaerobic process inwhichcarbsarebrokendown forenergyproduction endproducts Common offermentationare isAcidendproduct 2Acidand gasproduct Themediainthetube contains isphenolred pitindicaryell oninacidicmedia asinglecarb 3peptone sothebacteriacan eveniffermentationdoesn'toccur grow 4Durhamtube smallinvertedtubetodetect gasproduction Ifgasisproducedalongwithaciddurhamtubecollectsthe gasbubbles Note If thecurbisnotfermented thesolutionwillremainredanddoesn'tturnyellow