Starch Digestion and Salivary Amylase Quiz

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Questions and Answers

What is formed when phosphoric acid reacts with ammonium molybdate in the presence of HNO3?

  • A white precipitate
  • A red solution
  • A yellow precipitate (correct)
  • No precipitate forms

What occurs when KSCN is added to saliva and mixed with FeCl3 and HCl?

  • A blue precipitate forms
  • A colorless solution forms
  • No visible reaction occurs
  • A brick-red precipitate appears (correct)

What is the concentration range of SCN- ions found in saliva?

  • 0.01-0.03 mg% (correct)
  • 0.05-0.10 mg%
  • 0.50-1.00 mg%
  • 0.20-0.30 mg%

How does the concentration of KSCN in smokers' saliva compare to that in non-smokers' saliva?

<p>It is higher in smokers (C)</p> Signup and view all the answers

What product results from the proteic metabolism as indicated in the content?

<p>SCN- ions (D)</p> Signup and view all the answers

What is the final digestion product of starch?

<p>Maltose (C)</p> Signup and view all the answers

Which color indicates the presence of amylodextrines after adding Lugol solution?

<p>Blue-violet (A)</p> Signup and view all the answers

What temperature condition affects the activity of salivary amylase the most?

<p>Boiling temperature (A)</p> Signup and view all the answers

Which statement describes the effect of boiled saliva on starch digestion?

<p>It has no effect on the starch solution. (D)</p> Signup and view all the answers

What is observed after 30 minutes of starch digestion with fresh saliva?

<p>Colorless solution (D)</p> Signup and view all the answers

Which reagent is used to identify maltose in the digestion process?

<p>Trommer reagent (B)</p> Signup and view all the answers

What color does erythrodextrines produce when reacting with Lugol solution?

<p>Pink-violet (D)</p> Signup and view all the answers

What is the primary factor that influences salivary amylase activity?

<p>Temperature and pH (C)</p> Signup and view all the answers

What is the primary role of NaOH in the Trommer reaction with maltose?

<p>To maintain an alkaline environment (C)</p> Signup and view all the answers

What indicates the presence of calcium ions in saliva when treated with ammonium oxalate?

<p>White insoluble precipitate formation (D)</p> Signup and view all the answers

What is observed when maltose is reacted with CuSO4 solution in an alkaline pH during the Trommer reaction?

<p>Development of a red-copper precipitate (B)</p> Signup and view all the answers

Which of the following statements about phosphorus detection in saliva is correct?

<p>It involves the use of HNO3 and ammonium molybdate (D)</p> Signup and view all the answers

Why is boiling necessary during the Trommer reaction?

<p>To promote the formation of precipitates (C)</p> Signup and view all the answers

What type of crystals is associated with the presence of calcium oxalate precipitates in saliva?

<p>Octahedral crystals (B)</p> Signup and view all the answers

What might be a cause for a negative Trommer reaction when testing maltose?

<p>Test conducted in a neutral pH (A)</p> Signup and view all the answers

Which component is critical for the precipitation of phospho ammonium molybdate in saliva testing?

<p>HNO3 (A)</p> Signup and view all the answers

Flashcards

Ammonium phosphomolybdate formation

A chemical reaction involving sodium phosphate, ammonium molybdate, and nitric acid. It produces a yellow precipitate, which is ammonium phosphomolybdate.

Potassium sulfocyanate (KSCN)

A chemical compound with the formula KSCN, commonly found in saliva. It is a detoxification product resulting from protein metabolism or fruit ingestion.

Ferric sulfocyanate formation

A chemical reaction that occurs when potassium sulfocyanate (KSCN) reacts with ferric chloride (FeCl3) in the presence of hydrochloric acid (HCl). It produces a brick-red precipitate known as ferric sulfocyanate.

KSCN concentration in smokers vs non-smokers

The concentration of potassium sulfocyanate (KSCN) is higher in the saliva of smokers compared to non-smokers.

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Minerals evidence in saliva

This refers to the detectable presence of minerals within saliva. It can be analyzed using chemical reactions, like the formation of ferric sulfocyanate.

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Trommer's Reaction

A chemical reaction used to detect the presence of reducing sugars like maltose. Maltose reduces copper sulfate (CuSO4) in an alkaline solution, creating a red-copper precipitate when heated.

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Role of NaOH in Trommer's Reaction

Sodium Hydroxide (NaOH) is used to make the solution alkaline for the Trommer's reaction to occur.

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Red Precipitate in Trommer's Reaction

The red precipitate that forms at the bottom of the test tube indicates the presence of reducing sugars in the solution.

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Calcium Oxalate Precipitate

A white, insoluble precipitate that forms when saliva containing calcium ions is mixed with ammonium oxalate. It is composed of calcium oxalate crystals.

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Calcium Oxalate Crystal Shape

Under a microscope, calcium oxalate crystals appear as octahedrons, which are eight-sided geometric shapes.

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Yellow Precipitate in Phosphate Test

A yellow precipitate called ammonium phospho-molybdate appears when saliva is heated with nitric acid (HNO3) and ammonium molybdate. This indicates the presence of phosphate in saliva.

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Role of Nitric Acid in Phosphate Test

Nitric acid is used to create a suitable acidic environment for the phosphate test.

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Ammonium Molybdate in Phosphate Test

Ammonium molybdate is a reagent used in the phosphate test to react with phosphate ions in saliva, producing a yellow precipitate.

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What is the role of saliva in starch digestion?

Saliva contains an enzyme called ptyalin (also known as salivary amylase) that breaks down starch into simpler sugars.

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What are the intermediate products of starch digestion by saliva?

Starch is broken down into several shorter polysaccharides like amylodextrines, erythrodextrines, and acrodextrines. Ultimately, it's broken down into maltose, a disaccharide.

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How can we identify the different stages of starch digestion?

Lugol's solution (iodine and potassium iodide) is used to identify the different stages of starch digestion based on color changes. Blue-violet indicates amylodextrines, pink-violet indicates erythrodextrines, and a colorless solution indicates acrodextrines or maltose.

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What is the final product of starch digestion by saliva?

Maltose is the final product of starch digestion by saliva. It's a disaccharide, meaning it's made of two sugar units.

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Why does boiling saliva stop starch digestion?

Heating saliva inactivates the enzyme ptyalin, preventing further starch breakdown. In the experiment, boiling saliva before adding it to starch inhibits digestion.

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What factors influence the activity of salivary amylase?

Factors like temperature and pH affect the activity of ptyalin. Ptyalin works best at a specific temperature (optimal temperature) and pH (optimal pH). If conditions deviate from this ideal, the activity of the enzyme slows down.

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What does the time factor demonstrate in the starch digestion experiment?

The experiment uses different time intervals (2-3 minutes, 5-7 minutes, 15-20 minutes, and 30 minutes) to show the progressive breakdown of starch. This demonstrates the time-dependent nature of enzyme action.

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What is the purpose of the control tube (T1) in the starch digestion experiment?

The experiment uses a control tube (T1) with starch and Lugol's solution only, to compare the color change in other tubes with saliva. This helps to establish a baseline for the digestion process.

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Study Notes

Digestive Effect of Saliva on Starch

  • Starch digestion begins in the mouth, mixing with saliva containing ptyalin.
  • Starch breaks down into shorter polysaccharides (amylodextrins, erythrodextrines, acrodextrines), and finally maltose (a disaccharide).
  • Each step can be identified using Lugol's solution (identification reaction).
  • The final product of starch digestion is maltose, identifiable using the Trommer reaction.
  • Objective: Understand salivary amylase action, its demonstration, and factors influencing its activity (temperature, pH).

Materials

  • 1% Starch solution
  • 1% Iodine (Lugol's reagent) (1:2:300 iodine, potassium, water)
  • Test tubes
  • Graduated cylinders
  • Saliva
  • Sodium hydroxide (NaOH)
  • Copper(II) sulfate (CuSO4) 10% solution
  • Filter paper

Procedure

  • Use clean test tubes, adding 5ml of starch solution and 2-3 drops of Lugol's solution (control).
  • Experiment with saliva: boiled saliva (T2), fresh saliva (T3), fresh saliva, 5-7 min, then heat (T4), fresh saliva, 15-20 min, then heat (T5) and fresh saliva, 30 minutes (T6).
  • Observe color changes at each stage of starch digestion:
    • Amylodextrines: blue-violet (T3)
    • Erythrodextrines: pink-violet (T4)
    • Acrodextrines: colourless (T5)
    • Maltose: colourless (T6)

Trommer Reaction

  • Principle: Maltose reduces Copper(II) sulfate (CuSO4) to cuprous oxide in alkaline conditions.
  • Materials: Test tubes, Bunsen burner, NaOH 20% solution, CuSO4 10% solution, starch solution.
  • Procedure: Mix maltose solution and NaOH, add CuSO4 solution dropwise, generating a blue precipitate. Boil the solution to form a red-colored copper precipitate.

Calcium in Saliva

  • Materials: Test tube, ammonium oxalate 10% solution, saliva, microscope
  • Procedure: Add ammonium oxalate to saliva, forming a white precipitate in the presence of calcium. Examine octahedral crystals under a microscope (related to calcium oxalate formation).

Phosphorus in Saliva

  • Materials: Test tubes, gas burner, HNO3 (nitric acid), (NH4)2MoO4 (ammonium molybdate)
  • Procedure: Add HNO3 and ammonium molybdate to saliva. Heat carefully. Formation of a yellow precipitate indicates phosphorus (phospho ammonium molybdate).

Potassium Thiocyanate in Saliva

  • Materials: Test tubes, FeCl3 (ferric chloride) 3%, HCl (hydrochloric acid) 10% solution, saliva
  • Procedure: Add HCl and ferric chloride to saliva. A brick-red precipitate of ferric thiocyanate indicates the presence of potassium thiocyanate. Higher levels of thiocyanate are found in the saliva of smokers.

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