BIOL 251: Basic Microbiology - Culture Characteristics of Bacteria 2023/2024

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What is the key role of staining in microbiology?

To increase the size of bacterial colonies for better observation

To emphasize certain structures and enable the shape and arrangement of microbes to be appreciated (correct)

To enhance the taste of bacterial colonies for identification

To change the color of bacterial colonies to aid in differentiation

Which term describes the elevation of a bacterial colony?

Filamentous

Echinulate (correct)

Rhizoid

Viscid

What does a 'filiform' growth pattern in agar slants indicate?

Root-like appearance

Spindle shape

Treelike growth

Straight, thread-like growth (correct)

Which term describes a bacterial colony that is shiny and reflective?

Iridescent Signup and view all the answers

What indicates a 'punctiform' colony?

Pinpoint size Signup and view all the answers

In broth culture, what does a 'pellicle' indicate?

Floating membrane at the surface Signup and view all the answers

What is the main reason why basic dyes are attracted to bacterial cells?

Bacterial cells have negatively charged acidic components Signup and view all the answers

Which statement accurately describes acidic dyes?

They ionize and create an anionic color, working best in acidic pH. Signup and view all the answers

Why is fixation important in slide preparation for staining?

To prevent the specimen from washing away during the staining process Signup and view all the answers

What is the purpose of wet mounts in microscopy?

To observe live microorganisms without staining Signup and view all the answers

Which staining technique involves spreading microbes from a loopful of medium on a slide?

Smears Signup and view all the answers

Why do acidic dyes not stain bacterial cells but can stain background materials?

Due to the positively charged acidic components in bacterial cells Signup and view all the answers

BIOL 251: Basic Microbiology - Culture Characteristics of Bacteria 2023/2024

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Questions and Answers

What is the key role of staining in microbiology?

Which term describes the elevation of a bacterial colony?

What does a 'filiform' growth pattern in agar slants indicate?

Which term describes a bacterial colony that is shiny and reflective?

What indicates a 'punctiform' colony?

In broth culture, what does a 'pellicle' indicate?

What is the main reason why basic dyes are attracted to bacterial cells?

Which statement accurately describes acidic dyes?

Why is fixation important in slide preparation for staining?

What is the purpose of wet mounts in microscopy?

Which staining technique involves spreading microbes from a loopful of medium on a slide?

Why do acidic dyes not stain bacterial cells but can stain background materials?

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