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Pseudomonas aeruginosa Morphology and Culture Characters
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Pseudomonas aeruginosa Morphology and Culture Characters

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Questions and Answers

Which of the following is NOT a culture characteristic of Pseudomonas aeruginosa?

  • Grows anaerobically (correct)
  • Shows beta-hemolysis on blood agar
  • Produces sweet grape-like fruity odor
  • Gives greenish color to nutrient agar
  • What is the primary method for identifying Pseudomonas aeruginosa?

  • Fermentation test
  • Oxidase test (correct)
  • Catalase test
  • Gram staining
  • Which of the following is a virulence factor of Pseudomonas aeruginosa that causes tissue damage?

  • LPS
  • Exotoxin A (correct)
  • Pyoverdin
  • Pili
  • Which pigment is responsible for the nonfluorescent bluish color produced by Pseudomonas aeruginosa?

    <p>Pyocyanin</p> Signup and view all the answers

    What distinguishes the culture characteristics of Pseudomonas aeruginosa on nutrient agar?

    <p>$\beta$-hemolysis</p> Signup and view all the answers

    What type of bacteria are Pseudomonas aeruginosa based on their morphology?

    <p>Gram-negative rods</p> Signup and view all the answers

    How does P. aeruginosa become pathogenic?

    <p>By disrupting mucous membrane and skin</p> Signup and view all the answers

    What is a common clinical manifestation of P. aeruginosa infection in patients with severe burns?

    <p>Bacteremia</p> Signup and view all the answers

    Which antibiotic therapy is generally required to treat P. aeruginosa infections?

    <p>Aminoglycoside</p> Signup and view all the answers

    What is the most common predisposing factor for Moraxella catarrhalis infections?

    <p>Advanced age</p> Signup and view all the answers

    What is the color of Moraxella catarrhalis colonies on agar?

    <p>Grayish-white</p> Signup and view all the answers

    What is a characteristic of Moraxella catarrhalis in the laboratory diagnosis?

    <p>Non-motile</p> Signup and view all the answers

    Which treatment is effective for Moraxella catarrhalis infections?

    <p>Fluoroquinolones</p> Signup and view all the answers

    What is a common clinical infection caused by Moraxella catarrhalis?

    <p>Meningitis</p> Signup and view all the answers

    What is a characteristic of P. aeruginosa in laboratory diagnosis?

    <p>Oxidase positive</p> Signup and view all the answers

    What is a clinical manifestation of P. aeruginosa in pulmonary infection?

    <p>Necrotizing pneumonia in CF patients</p> Signup and view all the answers

    Study Notes

    Characteristics of Pseudomonas aeruginosa

    • Pseudomonas aeruginosa is a non-lactose fermenting gram-negative rod.
    • A primary method for identifying Pseudomonas aeruginosa is through its growth on cetrimide agar, leading to the formation of a distinct blue-green pigment.
    • A virulence factor that causes tissue damage is the production of elastase, which breaks down elastin in tissues.
    • The pigment responsible for the nonfluorescent bluish color is called pyocyanin.

    Culture Characteristics

    • On nutrient agar, Pseudomonas aeruginosa typically presents as rough, greenish colonies due to pigment production.
    • Morphologically, Pseudomonas aeruginosa are bacilli, appearing as rod-shaped bacteria.
    • Pathogenicity occurs through adherence to surfaces, evasion of host immune responses, and production of biofilms.

    Clinical Manifestations

    • Common clinical manifestations of Pseudomonas aeruginosa infections, especially in burn patients, include increased risk of sepsis and pneumonia.
    • A notable clinical manifestation in pulmonary infections is the production of a significant amount of mucus, leading to obstructive airway diseases.

    Treatment and Antibiotic Resistance

    • Antipseudomonal antibiotics, such as piperacillin-tazobactam or meropenem, are generally required for treating Pseudomonas aeruginosa infections.
    • This organism tends to exhibit high levels of antibiotic resistance, necessitating susceptibility testing.

    Moraxella catarrhalis Characteristics

    • The most common predisposing factor for Moraxella catarrhalis infections is underlying respiratory disease or conditions that affect the immune system.
    • Moraxella catarrhalis colonies appear opaque and often produce a creamy, white color on agar.
    • A characteristic laboratory diagnosis feature is that Moraxella catarrhalis is oxidase-positive and grows well on chocolate agar.
    • Effective treatment for Moraxella catarrhalis infections usually involves amoxicillin-clavulanate or other beta-lactam antibiotics.
    • Common clinical infections caused by Moraxella catarrhalis include otitis media and sinusitis.

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    Description

    Explore the morphology and culture characteristics of Pseudomonas aeruginosa with this quiz presented by Professor Dina Moustafa Abou Rayia from the Medical Microbiology and Immunology Department. Test your knowledge on the gram-negative rods, motility, aerobic growth, and exopigment production of Pseudomonas aeruginosa.

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