Week 8: Tests for Secondary Hemostasis PDF
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Uploaded by SubsidizedEternity
Institute of Health Technology, Dhaka
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Summary
This document provides details of tests for secondary hemostasis, including specimen collection procedures, activation causes, and influencing factors such as container type and sample temperature. It also analyzes factors like appropriate container usage, temperature control, and the impact of hemolysis on sample integrity. The summary touches upon the importance of maintaining optimal conditions for accurate coagulation testing.
Full Transcript
8 TESTS FOR SECONDARY HEMOSTASIS SPECIMEN COLLECTION IN HEMOSTASIS TESTING Primary concern is to prevent premature activation (falsely shortened tests) of the clotting process CAUSES OF ACTIVATION 1. Contamination with tissue thromboplastin TISSUE FACTOR IS released form injured cells and activates...
8 TESTS FOR SECONDARY HEMOSTASIS SPECIMEN COLLECTION IN HEMOSTASIS TESTING Primary concern is to prevent premature activation (falsely shortened tests) of the clotting process CAUSES OF ACTIVATION 1. Contamination with tissue thromboplastin TISSUE FACTOR IS released form injured cells and activates the EXTRINSIC PATHWAY ENDOTHELIAL DAMAGE F-III FVII FVIIA 2. Inappropriate container Glass provides a negatively charged surface activating the INTRINSIC PARTHWAY and attracts PLATELETS Use polystyrene tubes or glass tubes coated with silicone 3 Improper temperature FACTOR V AND VIII Labile factors at room temperature FACTOR VII AND XI Prematurely activated at ref temperature 4. Hemolysis Prolonged tourniquet application (
