Lecture 3:Genetics PDF

Warith Al-Anbiyaa University College of Medicine Lecture 3:Genetics Department Microbiology Dr Nisreen Jawad Objectives Students will be able to define mutation as a change in the DNA base sequence, s...

Warith Al-Anbiyaa University College of Medicine Lecture 3:Genetics Department Microbiology Dr Nisreen Jawad Objectives Students will be able to define mutation as a change in the DNA base sequence, state the potential effects of mutations on proteins produced as being beneficial, neutral, or harmful, recognize substitution, insertion, and deletion gene mutations, recognize duplication, inversion, and deletion chromosomal mutations, distinguish between spontaneous mutations and induced mutations.  In this lecture….. Mutation: Gene (point) Mutation Chromosomal Mutation Gene Mutation A gene mutation occurs when the nucleotide sequence of the DNA is altered and a new sequence is passed on to the offspring. The change may be either a substitution of one or a few nucleotides for others or an insertion or deletion of one or a few pairs of nucleotides.  Gene Mutation Genomes of bacteria exist on a single double-stranded circular DNA molecule that contains approximately 4000 kb of DNA and are regulated by operons, the majority of bacterial genes exist on one circular chromosome, there are other genetic elements within the bacterial genome. A mutation is a change in the nucleotide sequence and can create new cellular functionalities or lead to the dysfunction of others. Mutations can occur spontaneously or be caused by exposure to mutation-inducing agents. Gene Mutation A spontaneous mutation is one that occurs as a result of natural processes in cells, for example DNA replication errors. These can be distinguished from induced mutations; those that occur as a result of interaction of DNA with an outside agent or mutagen that causes DNA damage. Mutagens may be of physical, chemical, or of biological origin. Mostly they act on the DNA directly, causing damage which may result in errors during replication. Although, severely damaged DNA can prevent replication and cause cell death.  Gene Mutation Bacterial genes with similar functions often share one promoter (RNA polymerase binding site) and are transcribed simultaneously; this system is called an operon. Typical operons consist of several structural genes that code for the enzymes required for the pathway. Regulation occurs through transcription factors binding to a short sequence of DNA between the promoter region and the structural genes called an operator Gene Mutation Results of mutations can produce changes in structural or colony characteristics or loss in sensitivity to antibiotics. Some potential consequences of mutations are as follows: Auxotrophs: have a mutation which leaves an essential nutrient process dysfunctional. Resistant mutants: can withstand the stress of exposure to inhibitory molecules or antibiotics secondary to acquired mutation. Regulatory mutants: have disruptions on regulatory sequences like promotor regions. Constitutive mutants: continuously express genes that usually switch on and off as in operons.  Gene Mutation Mutation rates have been measured in a great variety of organisms, mostly for mutants that exhibit visible and prominant effects. Mutation rates are generally lower in bacteria and other microorganisms than in more complex species. In humans and other multicellular organisms, the rate typically ranges from about 1 per 100,000 to 1 per 1,000,000 gametes. There is, however, considerable variation from gene to gene as well as from organism to organism. Gene Mutation Mutations are of fundamental importance in molecular biology for several reasons: 1- Mutations are important as the major source of genetic variation that drives evolutionary change. 2- Mutations may have deleterious or (rarely) advantageous consequences to an organism. 3- Mutant organisms are important tools for molecular biologists in characterizing the genes involved in cellular processes.  Gene Mutation At the molecular level, in eukaryotes and prokaryotes the simplest type of mutation is a nucleotide substitution, in which a nucleotide pair in a DNA duplex is replaced with a different nucleotide pair. Mutations that alter a single nucleotide pair are called point mutations. Other kinds of mutations cause more drastic changes in DNA, such as expansions of trinucleotide repeats, extensive insertions and deletions, and major chromosomal rearrangements. Gene Mutation  Gene Mutation Gene Mutation Other kinds of mutations cause more drastic changes in DNA, such as expansions of trinucleotide repeats, extensive insertions and deletions, and major chromosomal rearrangements.  Gene (Point) Mutation Transitions and transversions can lead to: 1- silent mutation 2- missense mutation 3- nonsense mutation Silent mutations Nucleotide substitutions in a protein-coding gene may or may not change the amino acid in the encoded protein. Mutations that change the nucleotide sequence without changing the amino acid sequence are called synonymous mutations or silent mutations. Mutational changes in nucleotides that are outside of coding regions can also be silent. However, some noncoding sequences do have essential functions in gene regulation and, in this case, mutations in these sequences would have phenotypic effects.  Silent mutations Missense mutations Nucleotide substitutions in protein-coding regions that do result in changed amino acids are called missense mutations or nonsynonymous mutations. This type of mutation is a change in one DNA base pair that results in the substitution of one amino acid for another in the protein made by a gene. A change in the amino acid sequence of a protein may alter the biological properties of the protein.  Missense mutations Nonsense mutations A nucleotide substitution that creates a new stop codon is called a nonsense mutation. Because nonsense mutations cause premature chain termination during protein synthesis, the remaining polypeptide fragment is nearly always nonfunctional. A nonsense mutation is also a change in one DNA base pair. Instead of substituting one amino acid for another, however, the altered DNA sequence prematurely signals the cell to stop building a protein. This type of mutation results in a shortened protein that may function improperly or not at all. Nonsense mutations  Chromosomal mutation Chromosomes, which carry the hereditary material, or DNA, are contained in the nucleus of each cell. Chromosomes come in pairs, with one member of each pair inherited from each parent. The two members of a pair are called homologous chromosomes. Each cell of an organism and all individuals of the same species have, as a rule, the same number of chromosomes. Chromosomal mutation Chromosomes, which carry the hereditary material, or DNA, are contained in the nucleus of each cell. Chromosomes come in pairs, with one member of each pair inherited from each parent. The two members of a pair are called homologous chromosomes. Each cell of an organism and all individuals of the same species have, as a rule, the same number of chromosomes.  Chromosomal mutation Changes in the number, size, or organization of chromosomes within a species are termed chromosomal mutations, chromosomal abnormalities, or chromosomal aberrations. Changes in number may occur by the fusion of two chromosomes into one, by fission of one chromosome into two, or by addition or subtraction of one or more whole chromosomes or sets of chromosomes. Chromosomal mutation Changes in the structure of chromosomes may occur by inversion, when a chromosomal segment rotates 180 degrees within the same location; by duplication, when a segment is added; by deletion, when a segment is lost; or by translocation, when a segment changes from one location to another in the same or a different chromosome. These are the processes by which chromosomes evolve. Inversions, translocations, fusions, and fissions do not change the amount of DNA. The importance of these mutations in evolution is that they change the linkage relationships between genes. Genes that were closely linked to each other become separated and vice versa; this can affect their expression because genes are often transcribed sequentially, two or more at a time. Chromosomal mutation