Urea Hydrolysis Test PDF
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Uploaded by InstructiveHeliotrope2665
Hernandez
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Summary
This document details a laboratory procedure for identifying bacteria that produce the enzyme urease through a urea hydrolysis test. The procedure includes adding bacteria to sterile water with urea and incubating it at specific temperature. The results are visually observed. It covers the purpose, medium, procedure, theory and results of the test.
Full Transcript
Urea Hydrolysis Test Biol G210L Hernandez Urea Hydrolysis Test - Purpose: to ID bacteria that produce the enzyme urease (hydrolyzes - - urea) - Medium: sterile water + urea tablet - Procedure: 1. Add 3 – 4...
Urea Hydrolysis Test Biol G210L Hernandez Urea Hydrolysis Test - Purpose: to ID bacteria that produce the enzyme urease (hydrolyzes - - urea) - Medium: sterile water + urea tablet - Procedure: 1. Add 3 – 4 colonies of org into sterile water 2. Add urea tablet 3. Incubate at 37oC ~48 hours not critical Theory - Urea tablet contains: - Urea - Phenol red pH indicator - Some bacteria produce urease: Loading… Urease Urea Ammonia + CO2 Basic—detected by phenol red Results - + : Bright pink - - : Yellow/peach has to be bright prink
