Podcast
Questions and Answers
The purpose of the Urea Hydrolysis Test is to identify bacteria that produce the enzyme ______.
urease
The medium used in the Urea Hydrolysis Test consists of sterile water and a ______ tablet.
urea
After adding the urea tablet, the mixture should be incubated at ______ degrees Celsius for approximately 48 hours.
37
The Urea tablet contains phenol red, which acts as a ______ indicator.
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A positive result in the Urea Hydrolysis Test is indicated by a ______ color.
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Study Notes
Urea Hydrolysis Test
- Purpose: Identify bacteria that produce the enzyme urease, which hydrolyzes urea.
- Medium: Sterile water plus a urea tablet.
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Procedure:
- Add 3-4 colonies of the organism into sterile water.
- Add a urea tablet.
- Incubate at 37°C for approximately 48 hours. This time is not critical.
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Theory:
- The urea tablet contains urea and a phenol red pH indicator.
- Some bacteria produce urease.
- Urease catalyzes the breakdown of urea into ammonia and carbon dioxide.
- Ammonia is a base, increasing the pH and changing the phenol red indicator color.
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Results:
- Positive (+): Bright pink color change indicates urease production.
- Negative (-): Yellow or peach color indicates no urease production.
- The color must be bright pink for a positive result.
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Description
This quiz covers the Urea Hydrolysis Test aimed at identifying bacteria that produce the enzyme urease. The procedure, theory, and interpretation of results are detailed, emphasizing the color change associated with urease activity. Test your knowledge about the steps and outcomes of this microbiological assessment.
