The Cell Cycle.pdf

The CellII Cycle Cycle phases e GI Mitosis and Sphase ·Betweenwintermine cells are when to and big will cells into push a sps phase S Replication DNA · only So occurs If cells are going shortperiod wherecel's divide to to i as prepare Mitosis prophase · Metaphase · Anaphas · Telophase · Interphase H...

The CellII Cycle Cycle phases e GI Mitosis and Sphase ·Betweenwintermine cells are when to and big will cells into push a sps phase S Replication DNA · only So occurs If cells are going shortperiod wherecel's divide to to i as prepare Mitosis prophase · Metaphase · Anaphas · Telophase · Interphase How a · *8 · O · · · : Protein Activation/Inhibition Protein · Degradation · · Transcription activation/Inhibition · · Regulated ? transitions all these Of Combination · I s l u · * ⑤ => key components CDKCyclin · * cell cycle : get * CDK dependent kinases Activated · Cyclin ·When inhibit a - of is in a E-CDK2 present , and prepare for are for the Phosphorylate targets - O cyclin-nlt phosphorylates the a target phosphorylation no Cycline cycle cell bound binds + cak plate of the rid big regulator the · Differentones foreachphast hrough Ubiquitination · is instarts accumulatingbe Cy transcription and through degradations a reduced complexes · API CD ! Cyclin-Dependent-kinases · marorbriver of theeyeety N eLamina-Ex ar - · Nuclear Lamina is En Sustains supports and · homodimer , nuclear activation to other leads that finishes inactivation to underneath the nuclear membrane membrane through a cor protein consisting of The Question : Why doesn't CDK phosphorylate Because it's tightly regulated : while substrate Lamin A do only to , alphahelix long a Lamin A Answer sta a in each substrate the Duringmitoticprophase, Phosphorylates · - different are of Lamin A , of eachother to binas cok right the mostly made It's ↳ : substrates that of leads phosphorylation sometimes · throughout the so during dissambly the to leads which in of a globular the don a nuclear a a cycle ? cell Prophase Regulation CDK · Regulatedby orylation/de-phosphorylation · Types a of Cyclin Inactive Mitotic 7MPF Cyclin ↳ , THol Cyclin provide Cyclin CDK only phosphorylates recognizes a target differentcyins Regulates · substrate the Cyclin expressions are cell change increases affinity binding by regulates therefore what substrate when its cycle > - MpF Surface to CDK is both activated for CAK CDK and the phosphorylated by and CDK in substrate phase each bound to a cycling that Cyclins only expressed in Regulators are and target Tightly Regulated during · Who different bind that specificity substrate 2 Different Active 3 Substrate-binding * conformational · · ↑ Di YS Cyclin * [dC25 - certain periods and rapidly degrada ? highly Regulated during the cell cycle so appear they only in certain * kars notPhosphorytarget stages · Cyclin disappears by Polyubiquitination mitoticcyins from · Cyclin-CDK 62-m are transcribed during o during +o combinations at a certain leads which transit is only to degradation active anaphase can be inactive by the time presence START Es · ligase complexes Two main Es · · SCF : ligase and complexes Gl-Sphase and APC/1 : Metaphase transition - the cell Regulate the , SiCI cycle cell cycle : G2-M transitions anaphase transitions and anaphase to telophase is phosphorylated by active G, CDK. This. phosphorylation is I Ammon · a CD(20 Metaphase ⑳ to triggers Metaphase - Anaphase transition Anaphase imetaphekinetochoresconnectthe centromeres of each Sister chromat as Spindle · * its arentogether attentpotena Mechanisms - Inmetabase,Ano. chromatids 3 separate and mitosis triggers poly ubiquitination which rase leave will cohesion complex inhibitor securi. The of free and each Sister of separate chromatid other from each unravels pophysavetaattiguteophaand cyclin cytokinesi ll render n APCICacho ,& transitions Promotes entry · in Polyubiquitated : START I Mitosis by degrading APC inhibitors START · SCF targets promotes START entry by Yeast Human targets SCF SCF the degrading targets :. inhibitors SicI RB P21 p 27 , M but active Sate en a in · late el , sof inhibits A degrading E bck inhibitor of S-phase Cyclins before