Microbiology Tests PDF
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Uploaded by jada
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Summary
This document provides information on various microbiology tests, including their uses and how to identify specific bacteria like Salmonella Typhi. It explains the Widal test, Triple Sugar Iron test (or Multi test), precipitation test, agglutination test, and Cetrimide agar test with detailed use cases.
Full Transcript
+-----------------------+-----------------------+-----------------------+ | **Tests** | **Uses** | **Identify which | | | | bacteria** | +=======================+=======================+=======================+ | Wi...
+-----------------------+-----------------------+-----------------------+ | **Tests** | **Uses** | **Identify which | | | | bacteria** | +=======================+=======================+=======================+ | Widal Test | Used to diagnose | Salmonella Typhi. | | | typhoid fever. Blood | | | | test that measures | | | | the levels of | | | | antibodies again 2 | | | | starains of | | | | salmonella typhi. | | | | | | | | This is a serological | | | | test used to detect | | | | antibodies against | | | | **Salmonella typhi** | | | | in human blood. It's | | | | not applicable for | | | | diagnosing | | | | **Salmonella** in | | | | animals, nor is it | | | | useful for detecting | | | | non-typhoidal | | | | **Salmonella** | | | | species in dogs. | | +-----------------------+-----------------------+-----------------------+ | Triple Sugar Iron | It tests for | Salmonella | | Test or Multi test | fermentation, with or | | | | without gas | | | | production of | | | | glucose, sucrose and | | | | lactose. Also tests | | | | for hydrogen sulphide | | | | from amino acids. | | | | Uses a phenol red | | | | indicator. | | +-----------------------+-----------------------+-----------------------+ | Precipitation test | | | +-----------------------+-----------------------+-----------------------+ | Agglutination Test | When an antigen or an | Slide Agg- Identifies | | | inorganic particle is | cultures osf | | | mixed with antibody | salmonella and | | | in the presence of | shigella | | | electrolytes at a | | | | suitable temp. | Tube agg- Tests for | | | | enteric fever, | | | | brucellosis and | | | | thypus fevers. | +-----------------------+-----------------------+-----------------------+ | Cetrimide agar test | A selective medium | N**ot appropriate** | | | primarily used for | for detecting | | | the isolation of | **Salmonella** or | | | **Pseudomonas | diagnosing **enteric | | | aeruginosa**, not | fever** in dogs, as | | | **Salmonella**. | it is specifically | | | **Cetrimide** | used for identifying | | | inhibits the growth | **Pseudomonas | | | of most other | aeruginosa**. | | | bacteria, allowing | | | | **Pseudomonas | | | | aeruginosa** to | | | | thrive. It is useful | | | | when **Pseudomonas** | | | | infection is | | | | suspected, | | | | particularly in cases | | | | like wound | | | | infections, | | | | respiratory | | | | infections, or burn | | | | infections. | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+
