Protein Properties (Electrophoresis, Sedimentation, Amphoterism, Denaturation) - PDF
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Uploaded by LuxuriantManticore
Amity University
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Summary
This document covers various properties of proteins, including electrophoresis, sedimentation, amphoterism, and denaturation. It explains concepts like isoelectric point and the importance of pH in protein behavior. Its applications are highlighted, such as protein purification and maintaining protein stability in food and drugs.
Full Transcript
Isoelectric Point (pI): Each protein has a specific pH at which it carries no net charge, known as the isoelectric point. Below the pI, proteins gain protons and carry a positive charge, while above the pI, they lose protons and carry a negative charge. Importance of pH: The solubility...
Isoelectric Point (pI): Each protein has a specific pH at which it carries no net charge, known as the isoelectric point. Below the pI, proteins gain protons and carry a positive charge, while above the pI, they lose protons and carry a negative charge. Importance of pH: The solubility and stability of proteins often depend on pH, affecting their biological activity and interactions. For example, at the pI, proteins are less soluble and may precipitate. Applications: Understanding amphoterism is crucial in protein purification, where isoelectric focusing can separate proteins based on pI, and in food and drug formulations to maintain protein stability.
