Hematology 1 PRELIM LABORATORY TOPIC 2 PDF
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This document provides information on peripheral blood smear preparation, covering sources of specimens, methods, and considerations. It also includes information on different types of microscopy, such as bright field microscopy and oil immersion microscopy, used in hematology.
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Hematology 1 Clinical Instructors: DENNIS P. MONILLA, RMT PRELIM: LABORATORY TOPIC 2...
Hematology 1 Clinical Instructors: DENNIS P. MONILLA, RMT PRELIM: LABORATORY TOPIC 2 ROMILET D. DEL ROSARIO, RMT PERIPHERAL BLOOD SMEAR PREPARATION used to look for abnormalities in blood cells. Provides information on the number and shape of these cells, which can help doctors diagnose certainblood disorders or other medical conditions. SOURCES OF SPECIMENS 1.EDTA BLOOD For best results, blood smears should be made within _______after specimen collection Should be stored at 18°C to 25°C before preparing the smears Advantages of EDTA blood smear: ✓ ____________________________ ✓ ____________________________ ✓ ____________________________ Disadvantages:_______________________ Effect: 2.FREE FLOWING CAPILLARY BLOOD Best for evaluation of blood cell morphology Advantages: ✓ Made at the patient’s bedside ✓ Some artifacts may be prevented Disadvantages: ✓ Platelet clumping ✓ Only few smears can be made METHOD OF BLOOD SMEAR PREPARATION 1.Cover glass smear (____________) ✓ Two cover glass smears ✓ Used for bone marrow samples 2.Cover glass and slide (___________) ✓ Used for bone marrow samples 3.Spun smear ✓ Buffy coat smear → for platelet and WBC count (
