Packed Cell Volume (PCV) PDF

Packed Cell Volume (PCV) OR Hematocrit (Hct)  Packed Cell Volume (PCV) Or Hematocrit (Hct) The volume of the packed RBCS expressed as a percentage of the whole column of blood is the PCV. A decrease in the number or size of red cells also decreases the amount of...

Packed Cell Volume (PCV) OR Hematocrit (Hct)  Packed Cell Volume (PCV) Or Hematocrit (Hct) The volume of the packed RBCS expressed as a percentage of the whole column of blood is the PCV. A decrease in the number or size of red cells also decreases the amount of space they occupy, resulting in a lower hematocrit. An increase in the number or size of red cells increases the amount of space they occupy, resulting in a higher hematocrit.  Aim of the experiment Factors affecting PCV PCV↑↑ inpolycythemia & dehydration. 1. No. of RBC PCV↓↓ inanemia. 2. Shape of RBC The color of plasma may give an indication 3. Plasma volume about some illness, red (hemolysis), yellow (jaundice) and milky (hypercholesterolemia). Normal value: for: ♂ 40% -54%(47%) ◦ ♀ 36%- 47% (42%)  Methods Microhaematocrit : Electronic cell counting.method.method ◦Microhaematocrit method: Material and instruments: I. Microhaematocrit tube (capillary tube) 75 mm in length and 1 mm in diameter which contains heparin and shows a red ring at the end of the tube. II. Microhaematocrit centrifuge device. III. Plastic seal to seal one end of microhaematocrit capillary tube. IV. Microhaematocrit reader. Microhaematocrit tube (capillary tube) clay sealing material Microhaematocrit centrifuge Microhaematocrit reader  Procedur e 1. Filled blood approximately two-third into microhematocrit tube. Wipe any excess blood from the outside of tube. 2. Seal the one end of tube with clay material (should be at least 4 mm). 3. Centrifuge 11,000-12,000 rpm for 5 minutes by using microhematocrit centrifuge. Result of PCV  Clinical implications ◦PCV increases in polycythemia and this could be either: □Physiological High Altitude and extreme physical exercise or excitement or hypoxia. □Pathological Polycythemia Vera, Dehydration leading to Hemoconcentration e.g. diarrhea, burns, and vomiting, Congenital heart failure, and Severe chronic obstructive pulmonary disease(COPD).  Clinical implications ◦PCV decreases in : 1. Anemia. 6. Bone marrow failure 2. Normal pregnancy. 7. Renal diseases. 3. Hemoglobinopathies 8. Autoimmune diseases.. 9.Malignancies like lymphoma, 4. Cirrhosis. leukemia, others 5. Hemorrhage.