Microscopy Lecture Notes PDF

Click to edit Master title style Edit Master text styles Second level Microscopy Third level Fourth level Fifth level Dr. SUZI SOBHY ATALLA Professor of Histology...

Click to edit Master title style Edit Master text styles Second level Microscopy Third level Fourth level Fifth level Dr. SUZI SOBHY ATALLA Professor of Histology 1 Click to edit Master title style EditRegarding these Master text two photos, styles Second level Third level Fourth level Fifth level What is the quality of an image examined by light microscope termed ( its clarity and details) depend on? 2 Click to edit Master title style Answer **It EditisMaster termedtext resolution, styles and it depends on the microscope's resolving Secondpower level Third level Fourth level Fifth level 3 Click to edit Master title style 1.Edit Master Define text styles the units of measurement commonly used in histology Second and level resolving power(resolution) the term Third level 2. Recognize level different parts of ordinary light in brief Fourth microscope(LM) & electron microscopes(EM) Fifth level 3. Differentiate between resolution and magnification of the microscope 4. Explain the terms electron dense and electron lucent areas 5. Compare between light and transmission and scanning electron microscopes Extended Modular Program 4 Click to edit Master title style Commonly Used International Units of Measurement Edit Master text styles in Histology Second level Third level 1 micrometer Fourth level (um) = 1/1000 millimeter (mm)or10-6m Fifth level 1 nanometer (nm) = 1/1000um = 10-6mm = 10-9m 5 ClickParts to edit Master title style of the Ordinary Light Microscope(LM) Edit LMMaster textto is used styles examine stained sections Second level by Third level natural or electric light Fourth level as a source of Fifth level illumination. LM includes (1) Optical part (2) Mechanical part 6 Click Optical 1-The to editPart Master of the title style Light Microscope Consists of three lenses: Edit Master text styles (1) Condenser lens Second level (2) Objective Third level lens Fourth level (3) Ocular lens or Fifth level eyepiece 7 Click (1) Condenser to edit Master title style Edit Master text styles Second level Function: Third level Fourth level collects & focuses the Fifth level light that illuminates the section to be observed 8  Click (2)to edit Master Objective Lenstitle style Attached Edit Master to thetext styles rotating nosepiece having different Second levelmagnification powers Third level X10= low power Fourth level Fifth level X40=high power X100=oil immersion Function: Enlarges & projects image to ocular lens Responsible for good resolving power of the microscope 9 Click to edit Master title style (3) Ocular Lens Edit Master With text styles different magnification powers inscribed on the Second level eyepiece Third level Fourth level X5, X10, X15 Fifth level Function: Magnifies & projects the image onto viewers retina 10 Click to edit Master title style 2-Mechanical part Edit Master text styles Second level Third level Fourth level Fifth level Move and focus the examined section 11  Parts of LM Function of Each Part Click to (1) Optical Part edit Master *To focus title light & style magnifies image of examined section Edit Master a-Condenser text styles *Collects & focus light to illuminate section Second level Third level b-Objective lens Fourth level *Enlarges & projects image to ocular lens Fifth level c- Ocular lens *Magnifies image & projects onto viewers retina (2) Mechanical Part *To move & focus the examined section 12 Click to edit Master title style Resolution Deinition : Edit Master text styles The smallest distance between two adjacent Second level particles at which they can be seen as separate objects. Third level The Resolving Power (RP) of the LM is 0.2um Fourth level Fifth level The resolving power of the eye is 0.2 mm Resolving power depends on quality of objective lens N.B: the ocular lens magnifies only the images from objective and does not improve the resolution 13 Click to edit Master title style Magnification Power (MP) of Microscope Edit Master text magnification - The total styles = Second level MP of objective lens X MP of ocular lens X Third level Fourth level Fifth level - MP of LM= 1000- 1500 times - Magnification is independent of RP , but is of value when accompanied with high resolution 14 Click to edit Master title style Edit Master text styles Second level Third level Fourth level Fifth level 1- What is the function of the condenser lens? How to use the microscope: https://youtu.be/SUo2fHZaZCU 15 Click to edit Electron Transmission Master Microscope(TEM title style ) Edit Master text styles Second level Third level Fourth level Fifth level 16  Principle Click to editof TEM title style Master Edit Masteroftext Source styles is a beam of illumination electrons Second level Third level (1) Electromagnetic lenses that detect beam of Fourth level electrons are: Fifth level (1) Condenser focuses electron beams (2) (2) Objective lens magnifies the image (3) Projector lens magnifies images Magnified image project on 4) Fluorescent screen 17 Click to edit Master title style TEM Used to study fine structures with high resolution(0.1nm) Edit Master text styles Second level Allows Thirdvery level thin sections Fourth level (ultrathin sections) less than Fifth level 1um to be observed in details at magnifications up to 120,000 times 18 Click oftoThe Color edit Master Resulting titlewith Image style TEM Edit Master text styles Why Second images appears level black Third and level white? Fourth level Electron Fifth dense level areas of the specimen appear black as they scatter the beam of electrons Electron lucent areas of the specimen appear white as they allow electrons to pass through. 19 Click to edit of Preparation Master tissues title forstyle Electron Microscope 2. Fixation Edit Master text styles 1. Tissue sample 4%glutaraldehyde 1% 3. Dehydration