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Questions and Answers
What is the quality of an image examined by a light microscope termed?
What is the quality of an image examined by a light microscope termed?
resolution
What are the units of measurement commonly used in histology for resolving power?
What are the units of measurement commonly used in histology for resolving power?
micrometer and nanometer
Which of the following are parts of an ordinary light microscope?
Which of the following are parts of an ordinary light microscope?
- Mechanical part (correct)
- Optical part (correct)
- Electrical part
- Optical and mechanical parts (correct)
What are the three lenses that make up the optical part of the light microscope?
What are the three lenses that make up the optical part of the light microscope?
What is the function of the condenser lens?
What is the function of the condenser lens?
What magnification powers are available for the objective lens?
What magnification powers are available for the objective lens?
What does the ocular lens do?
What does the ocular lens do?
What is the purpose of the mechanical part of the microscope?
What is the purpose of the mechanical part of the microscope?
Resolution and magnification are the same.
Resolution and magnification are the same.
Define electron dense and electron lucent areas.
Define electron dense and electron lucent areas.
Compare between light microscopes and electron microscopes.
Compare between light microscopes and electron microscopes.
Flashcards
Resolution (Image quality)
Resolution (Image quality)
The ability of a microscope to distinguish between two closely spaced points. It determines the sharpness and clarity of details visible in an image.
Micrometer and Nanometer
Micrometer and Nanometer
Units of measurement commonly used in histology to express the resolving power of microscopes. A micrometer is a millionth of a meter, and a nanometer is a billionth of a meter.
Condenser Lens
Condenser Lens
A lens in a light microscope that focuses and collects light from the light source, directing it towards the specimen.
Objective Lens
Objective Lens
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Ocular Lens
Ocular Lens
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Mechanical Part (Microscope)
Mechanical Part (Microscope)
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Electron Dense Area
Electron Dense Area
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Electron Lucent Area
Electron Lucent Area
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Light Microscope
Light Microscope
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Electron Microscope
Electron Microscope
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Resolution vs. Magnification
Resolution vs. Magnification
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Study Notes
Microscopy Overview
- Resolution defines the clarity and detail of an image in microscopy, dependent on the resolving power of the microscope.
Measurement Units in Histology
- Commonly used units include:
- 1 micrometer (µm) = 1/1000 millimeter (mm) or 10^-6 meters (m)
- 1 nanometer (nm) = 1/1000 µm = 10^-6 mm = 10^-9 m
Light Microscope (LM) Components
- Comprises two main parts:
- Optical part: responsible for focusing light and magnifying the image.
- Mechanical part: assists in moving and focusing the specimen.
Optical Part Components
- Consists of three types of lenses:
- Condenser Lens:
- Collects and focuses light for specimen illumination.
- Objective Lens:
- Attached to the rotating nosepiece with varying magnification powers (Low: X10, High: X40, Oil immersion: X100).
- Enlarge and project images while contributing to resolving power.
- Ocular Lens (Eyepiece):
- Features various magnifications (X5, X10, X15).
- Magnifies and projects the image onto the viewer's retina.
- Condenser Lens:
Functions of Light Microscope Parts
- Optical Part Function:
- Focuses light and magnifies the image of the specimen.
- Mechanical Part Function:
- Facilitates movement and precise focusing of the examined specimen.
Electron Microscopes (EM)
- Differ from Light Microscopes (LM) due to the use of electron beams for imaging, offering higher resolution but with distinct operational differences.
Key Concepts
- Differentiate between resolution (clarity of image) and magnification (size of image).
- Understand terms:
- Electron dense: areas that block electrons in imaging.
- Electron lucent: areas that allow electrons to pass through.
Comparison of Microscopes
- Light Microscopes (LM) utilize visible light, while:
- Transmission EM: passes electrons through thin specimens.
- Scanning EM: scans the surface of specimens for 3D images.
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