Gram Staining Questions PDF

Prof. AboRashad 00201012073853 - 0535799855 - X- @DrAborashad22 552 687 Question Image Questions ID Which scientist developed the Gram staining 1 technique?Prof.Dr.AboRashad...

Prof. AboRashad 00201012073853 - 0535799855 - X- @DrAborashad22 552 687 Question Image Questions ID Which scientist developed the Gram staining 1 technique?Prof.Dr.AboRashad a-Hans Christian b-Robert Koch c-Robert Koch d-Antonie van Leeuwenhoek What is the purpose of the alcohol or acetone in Gram 2 staining?Prof.Dr.AboRashad a- To fix the cells b-To decolorize the cells c-To enhance the contrast d-To visualize bacterial capsules What is the primary stain used in Gram 3 staining?Prof.Dr.AboRashad a-Crystal violet b-Safranin c-Methylene blue D-Iodine Prof.Dr.AboRashad What can cause Gram-positive bacteria to appear Gram- 4 negative? Prof.Dr.AboRashad a-Over-staining with crystal violet b-Using an old bacterial cult c-Incomplete fixation d-Cell wall damage What happens if the decolorization step is skipped 5 during Gram staining? Prof.Dr.AboRashad a-Gram-positive bacteria will appear as G ve- b-Gram-negative bacteria will appear as Gram+ c-The stain will not adhere to the bacterial cells. d-The bacterial cells will be over-stained 1 Which bacteria appear as gram negative kidney bean 6 shaped diplococci Prof.Dr.AboRashad a-Staph aureus b-Strepto pyogenes c-H.influnza d-Listeria e-Neisseria spp. Which bacteria is Gram-negative curved, comma shaped 7 short rods a-Campylobacter b-Vibro cholera c-Neisseria gonorrhea d-Salmonella Prof.Dr.AboRashad Whic bacteria Gram-negative curved, thin, rods (S- 8 shaped, seagull shaped or corkscrew shaped) Prof.Dr.AboRashad a-Legionella spp b-Pseudomonas c-Haemophilus spp d-Campylobacter spp Which is Gram-negative rods with bipolar staining 9 ("closed safety pin) a-Yersinia pestis Prof.Dr.AboRashad b-Legionella spp c-Enterococcus spp d-Pseudomonas A sputum sample , Gram positive cocci which corelates 10 with bacterial cell wall properties. Which of the following is false regarding the bacterial cell wall? a-Cell wall of Gram-positive bacteria is thicker than that Gram-negative bacteria b-Teichoic acid is present in cell wall of Gram -ve c- Region of LPS is core polysaccharide d-LPS is responsible for O antigen specificity of gram negative bacteria 2 Which factor can lead to a Gram-negative organism 11 appearing Gram-positive?Prof.Dr.AboRashad a-A smear that is too thin b-Organisms are fully decolorized Prof.Dr.AboRashad c-A smear that is too thick d-Use of a new iodine solution Which can stain by fit modified acid fast stain? 12 a-Nocarida b-Cryptosporidium c-Cyclospora d-Cysstospora e-Leigionella f-All Prof.Dr.AboRashad The main differences between Zn and kinyoun? 13 a-Aconc.phenol and heat b-Primary stain c-counter stain d-Time of stain Prof.Dr.AboRashad What is the concentration of strong hydrochloric acid 14 HCL used for decolorization in Zn stain? a-%3 b-20% c-%5 d-0.5% Which counterstain in kinyoun stain?Prof.Dr.AboRashad 15 a-Crystal violot b-Methyl red Prof.Dr.AboRashad c-Carbol fuchsin d-methylen blue Malachite green uses in zn stain as?Prof.Dr.AboRashad 16 Decolorize Primary stain Mordant Counterstain 3 Primary stain used in endospore staining? 17 a-Malachite green 0.5% b-Distilled water c-Safranin Prof.Dr.AboRashad Schaeffer-Fulton method used in detection of? 18 a-Capsule b-Flagella Prof.Dr.AboRashad c-Cell wall d-Endospore What is the leifson stain used for?Prof.Dr.AboRashad 19 a-H.pylori b-S.pyogenes c-S.aureus d-Vibrocholera Which media used in general bacteriology, supports the 20 growth of most bacteria, with exceptions (eg, N gonorrhea, H influenzae & Legionella)? a-SBA b-MAc c-Neut. d-Chocolate Which media Cultivation/isolation of fastidious bacteria 21 like Neisseria spp & Haemophilus spp, but not Legionella spp a-BCYE b-Muller hinton c-Blood agar d-Chocolate agar 4  Which Recovery of Legionella spp contains cysteine& 22 iron supplementation, necessary to support growth of Legionella Activated charcoal helps bind & sequester growth inhibitors that may be present in the specimen? a-L J b-MACc c-Buffered charcoal yeast extract (BCYE) agar d-Blood agar Which media contain Bile salts & crystal violet 23 inhibit growth of gram-positive bacteria & delicate gram-negative bacteria a-Blood agar b-MAc c-Thyer martin d-TCBS Media Cultivation isolation of hardy enteric gram 24 negative rods a-RMBC AboRashad b-EMB c-AMB d-RMA Bile salts that inhibit gram positive bacteria and 25 Alkaline pH inhibits most enterics & enhances growth of which of the following bacteria?Prof.AboRashad a-E.coli b-Salmonella c-Listeria d-Vibro Media cntain antimicrobials to which Bordetella spp are 26 resistant (cephalexin)?Prof.AboRashad a-Region low b-Neutrient c-MaCc Prof.AboRashad d-TCBC 5  Which media contain antimicrobials to which Neisseria 27 spp are resistant (vancomycin, colistin, nystatin & SXT) Recovery of Neisseria spp from nonsterile sites? a-Thyer martin b-Macc c-TCBS Prof.AboRashad d-EMB The localized nature of S. aureus lesions is due to? 28 a-Adhesins b-Protein A c-Staphylocoagulase Prof.AboRashad d-Staphylokinase e-Catalase The following statements about S.aureus food poisoning 29 are true EXCEPT? Prof.AboRashad a-It is caused by enterotoxins. b- Source of contamination is carrier. c-The incubation period is 24-36 hours. d-Food contains preformed toxin. e-The responsible toxin acts as a superantigen. MRSA isolates are treated empirically by? 30 a-Erythromycin b-Vancomycin c-Clindamycin d-Cephalosporins Prof.AboRashad e-Tetracycline The most important factor enabling S. epidermidis to 31 colonize prosthetic devices is:Prof.AboRashad a-Production of coagulase b-Resistance to many antibiotics c-Production of glycocalyx d-Production of clumping factor e-Production of exotoxin 6 An emergency department physician ordered a culture 32 and sensitivity test on a catheterized urine specimen obtained from a 24-year-old female patient. A colony count was done and gave the following results after 24h. Blood agar plate = >100,000 col/mL of gram-positive cocci resembling staphylococci MacConkey agar = No growth CNA plate = Inhibited growth Hemolysis =Negative Catalase = Positive Novobiocin= Resistant This Isolate is:Prof.AboRashad a-Staphylococcus saprophyticus b-Micrococcus luteus c-Staphylococcus aureus d-Streptococcus pyogenes Which staining sequence should be used to stain the 33 smear of the sputum sample collected from a 32-year- old laborer with fever and hemoptysis, who works at a construction site?Prof.AboRashad a-Gentian violet – Iodine – alcohol saffranin b-Methylene blue-malachite green-A.acid-water c-Methanol – methylene blue-acid – water d-Carbol fuschin – acid alcohol- methylene blue A patient tested positive twice for HCV Antibody tests 34 that have different principles, but tested negative for HCV RNA testing. What is the interpretation? a-The patient is currently infected with HCV. b-The patient was infected with HCV in the past but has cleared the infection. c-The patient never been infected with HCV. d-The patient’s test results are inconclusive. At what temperature are complement in human serum 35 inactivated? Prof.AboRashad a-37°C b-56°C c-100°C d-121°C 7 Which organization in Saudi Arabia is responsible for 36 accrediting medical laboratories and ensuring compliance with quality standards?Prof.AboRashad a-(CBAHI) b-(SFDA)Prof.AboRashad c-(SAC) d-All Which of the following is the appropriate action if a CSF 37 sample was stained, reported to the patient, but later discovered to be incorrect?Prof.AboRashad a-Keep the mistake confidential to avoid causing unnecessary concern to the patient. b-Inform the patient about the error and offer a sincere apology. c-Disregard the mistake as it does not have any clinical significance. d-Delay informing the patient until further confirmation is obtained. What is the diagnosis of N. gonorrhoeae in males? 38 a-Urethritis b-Pharyngitis c-Conjunctivitis d-Proctitis Prof.AboRashad Which of the following are primary modes of 39 transmission for Brucella abortus bacteria except? a-Contact with Infected Cattle b-Consumption of Contaminated Animal Products milk..... c-Inhalation of Airborne Agents:Prof.AboRashad d-Contact with Infected goat and sheep 8 What color does Mycobacterium tuberculosis appear in 40 the Ziehl-Neelsen stain? Prof.AboRashad a-Blue b-Green Prof.AboRashad c-Red d-Purple What test is used for identifying Streptococcus 41 pneumoniae? a-Coagulase test b-Catalase test Prof.AboRashad c-Optochin sensitivity test. d-Bacitracin sensitivity test What is the ONPG test used for? 42 a-Detecting antibiotic resistance b-LF and NLF c-LLF and NLL d-Measuring oxidative phosphorylation When dealing with two beta-hemolytic bacteria, which 43 test would you perform to differentiate between them? Prof.AboRAshad a-Bactracin test. b-Optichin test c-Oxidase test Prof.AboRashad d-Urease test For which bacteria is the CAMP test used 44 a-Streptococcus agalactiae. b-Staphylococcus aureus c-Escherichia coli d-Pseudomonas aeruginosa 9 What is the indicator used in the candle jar method for 45 creating anaerobic conditions in a laboratory? a-Bromothymol blue b-Methylene blue c-Phenolphthalein d-Litmus paper Which bacteria cause meningitis in infants and 46 immunocompromised patients?Prof.AboRashad a-Streptococcus pneumoniae b-Pseudomonas aeruginosa c-Staphylococcus aureus d-S.pyogenes Which organism give this results in KIA test K/K, Gas – 47 ve, H2S – ve? a-Salmonella Typhi b-Vibrio cholerae c-Pseudomonas aeruginosa d-Proteus vulgaris What is the sample collection method used for testing 48 diphtheria using a sample from the throat?Prof.AboRashad a-Blood sample b-Sputum sample c-Throat swab sample d-Urine sample Which bacterial strain is associated with the 49 characteristic “double zone” hemolysis on blood agar, also known as target hemolysis?Prof.AboRashad a-Streptococcus pneumoniae b-Clostridium perfringens c-S.viridans d-Enterococcus 10 Which of the following bacteria cant grow in traditional 50 culture media? AboRashad a-Chlamydia species b-Rickettsia species c-Treponema pallidium d-All Prof.AboRashad e-Non 11