Summary

This document appears to be a laboratory report on Gram staining, focusing on identifying different types of bacteria. It includes questions about the process and requested results in a table format.

Full Transcript

**Gram staining** **EXERCISE 2 LABORATORY REPORT Student name: \-\-\-\-\-\-\-\-\-\-\--** **Students section: \-\-\-\-\-\-\-\-\-\-\-\--** **Purpose (One Mark):** **....................................................................................\...\...\...\...\....** **.........................

**Gram staining** **EXERCISE 2 LABORATORY REPORT Student name: \-\-\-\-\-\-\-\-\-\-\--** **Students section: \-\-\-\-\-\-\-\-\-\-\-\--** **Purpose (One Mark):** **....................................................................................\...\...\...\...\....** **................................................................................................** **Results:** **A: Complete the following table (2.5 Marks)** **Requested** **E.Coli** **S.aurus** ---------------------------- ------------ ------------- **Draw at 1000X or 100 X** **Morphology** **Arrangement** **Color** **Gram reaction** **B: Questions** 1. **How staining used to differentiate between the types of bacteria? (One Marke)** 1. **Identify the role of the following items used in staining techniques (2.5 Marks):** a. Mordant: b. The primary stain: c. 95% Ethyl alcohol: d. The Counterstain: e. 95% Methyl alcohol: 2. **Why should be Immediately washed the slide gently with distilled water after adding 95% Ethyl alcohol? (One Mark)** 3. **Determine the reasons for the following results:** a. Slide with a smear of gram-positive bacteria appearing under a microscope in pink color? (One Mark). b. Slide with a smear of gram-negative bacteria appearing under a microscope in violet color?(One Mark) c. Absence of bacteria on the slide under a microscope? (One Mark)

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