Lab 3 - DNA Extraction PDF
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This document discusses various methods for DNA extraction, including mechanical, chemical, and enzymatic processes. It outlines the steps involved in each method and covers the key principles behind solid-phase extraction and alcohol precipitation. The document also includes a summary for laboratory reports, outlining the expectations for such a report.
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Lab 2: DNA Extraction DNA Extraction Steps 1. Cell Lysis 2. Separation of DNA - Purification and/or Precipitation Cell Lysis Mechanical/ Physical Methods - Shearing and Grinding - localized heating Reagent based: Chemical (Detergents, Alkaline) Enzymatic (Proteinase K) Separation Conventional...
Lab 2: DNA Extraction DNA Extraction Steps 1. Cell Lysis 2. Separation of DNA - Purification and/or Precipitation Cell Lysis Mechanical/ Physical Methods - Shearing and Grinding - localized heating Reagent based: Chemical (Detergents, Alkaline) Enzymatic (Proteinase K) Separation Conventional method - Phenol/chloroform/isoamyl alcohol Solid-Phase Extraction Precipitation or Elution Elution of DNA from filter Alcohol precipitation a. b. c. Neutralize DNA with salt (CH3COONa, NaCl, LiCl) Addition of alcohol Centrifuge Summary Conventional Method Summary Solid-Phase Extraction Method Summary Alkaline Lysis Method Assessments: Lab Report VLE: “How to Write a Lab Report” Due Date: Thursday, March 13th 2023 Expectations: 1. Introduction 2. Methodology 3. Results and Discussion (Figures, Captions) 4. Conclusion 5. References
