IMA QUESTION BANK sessional 2.docx

IMA QUESTION BANK MCQ **1. Which of the following is the mobile phase in Gas Chromatography (GC)?** - A\) Solid - B\) Liquid - C\) Gas - D\) Plasma\ **Answer:** C) Gas **2. What is the primary purpose of the stationary phase in HPLC?** - A\) To separate components based on pola...

IMA QUESTION BANK MCQ **1. Which of the following is the mobile phase in Gas Chromatography (GC)?** - A\) Solid - B\) Liquid - C\) Gas - D\) Plasma\ **Answer:** C) Gas **2. What is the primary purpose of the stationary phase in HPLC?** - A\) To separate components based on polarity - B\) To heat the sample - C\) To dissolve the analyte - D\) To provide a mobile phase\ **Answer:** A) To separate components based on polarity **3. Which detector is commonly used in HPLC?** - A\) Flame Ionization Detector (FID) - B\) UV-Vis Detector - C\) Electron Capture Detector (ECD) - D\) Thermal Conductivity Detector (TCD)\ **Answer:** B) UV-Vis Detector **4. In GC, what does the retention time indicate?** - A\) The amount of sample injected - B\) The time taken for the sample to vaporize - C\) The time taken for a compound to pass through the column - D\) The volume of gas used\ **Answer:** C) The time taken for a compound to pass through the column **5. In reverse-phase HPLC, the stationary phase is:** - A\) Polar - B\) Non-polar - C\) Hydrophilic - D\) Ionizable\ **Answer:** B) Non-polar **6. What is the function of the column in GC?** - A\) To detect the analyte - B\) To cool the analyte - C\) To separate the components of the sample - D\) To generate the mobile phase\ **Answer:** C) To separate the components of the sample **7. Which of the following factors primarily affects the retention time in HPLC?** - A\) Injection volume - B\) Flow rate of the mobile phase - C\) Detector sensitivity - D\) Column temperature\ **Answer:** B) Flow rate of the mobile phase **8. In Gas Chromatography, what type of compounds are best suited for analysis?** - A\) High boiling point compounds - B\) Non-volatile compounds - C\) Volatile compounds - D\) Ionic compounds\ **Answer:** C) Volatile compounds **9. Which of the following is a common type of stationary phase coating used in Gas Chromatography?** - A\) Polyethylene Glycol (PEG) - B\) Silver nitrate - C\) Silica Gel - D\) Copper sulfate\ **Answer:** A) Polyethylene Glycol (PEG) ### **10.What is the primary purpose of ion exchange chromatography?** - A\) Separation of non-polar compounds - B\) Separation of ionic compounds - C\) Size exclusion of molecules - D\) Separation of volatile compounds\ **Answer:** B) Separation of ionic compounds ### 11. **What are ion exchange resins composed of?** - A\) Solid silica particles - B\) Organic polymers with charged functional groups - C\) Metal oxides - D\) Non-polar hydrocarbons\ **Answer:** B) Organic polymers with charged functional groups ### 11. **In ion exchange chromatography, what is the stationary phase typically composed of?** - A\) Charged particles - B\) Neutral beads - C\) Non-porous materials - D\) Gaseous molecules\ **Answer:** A) Charged particles ### 12. **Which of the following factors affects the ion exchange process the most?** - A\) Temperature - B\) Charge of the ions - C\) Detector type - D\) Sample size\ **Answer:** B) Charge of the ions ### 13. **Which of the following ions are typically exchanged during cation exchange chromatography?** - A\) Negatively charged ions - B\) Neutrally charged molecules - C\) Positively charged ions - D\) Large macromolecules\ **Answer:** C) Positively charged ions ### 14. **In ion exchange chromatography, the selectivity of the resin is influenced by:** - A\) Flow rate of the mobile phase - B\) Ion concentration in the solution - C\) Size of the column - D\) Surface tension of the liquid\ **Answer:** B) Ion concentration in the solution ### Gel Chromatography ### 15. **What is the principle behind gel chromatography?** - A\) Separation based on the ionic charge - B\) Separation based on molecular size - C\) Separation based on volatility - D\) Separation based on solubility\ **Answer:** B) Separation based on molecular size ### 16. **Which of the following materials is commonly used as a stationary phase in gel chromatography?** - A\) Agarose or dextran gels - B\) Silica beads - C\) Activated carbon - D\) Cellulose filters\ **Answer:** A) Agarose or dextran gels ### 17. **In gel filtration chromatography, smaller molecules travel through the column:** - A\) Faster than larger molecules - B\) At the same rate as larger molecules - C\) Slower than larger molecules - D\) Are completely excluded\ **Answer:** C) Slower than larger molecules ### Affinity Chromatography ### 18. **What is the key factor that allows separation in affinity chromatography?** - A\) Molecular size - B\) Electrostatic attraction - C\) Specific interaction between biomolecules - D\) Ionic strength\ **Answer:** C) Specific interaction between biomolecules ### 19. **Which of the following ligands can be used in affinity chromatography?** - A\) Antibodies - B\) Simple sugars - C\) Organic solvents - D\) Non-polar hydrocarbons\ **Answer:** A) Antibodies ### 20. **In affinity chromatography, the primary mechanism of separation is:** - A\) Molecular size exclusion - B\) Specific binding between the target molecule and the ligand - C\) Charge-based separation - D\) Phase transition\ **Answer:** B) Specific binding between the target molecule and the ligand ### 21. **Which type of biomolecule is often purified using affinity chromatography?** - A\) Nucleotides - B\) Proteins - C\) Lipids - D\) Carbohydrates\ **Answer:** B) Proteins ### 22. **What is the common technique used to elute the bound molecules in affinity chromatography?** - A\) Increasing temperature - B\) Using a buffer with a high salt concentration or pH change - C\) Introducing organic solvents - D\) Reducing the pressure\ **Answer:** B) Using a buffer with a high salt concentration or pH change 1\) a) Enlist four detectors used in Gas chromatography. Explain anyone detector in detail. Support your answer with suitable diagram. 5M b\) classify different types of ion exchange resin. Give suitable examples of each type 2\) a) Give an account of column packing which can be used in gel chromatography. Discuss application of Gel chromatography. b\) Enlists various types of columns used in HPLC. Give diagram of rheonyde injector with 'load' and 'inject' position. 5M **3)** a) Discuss electrophoretic mobility. Discuss in detail factor affecting the same. 5M b\) Give Principal of Ion Exchange chromatography. classify different types of ion exchange resin. Give suitable examples of each type. 5 M SAQ 1)Discuss guard column in HPLC. Explain the term isocratic elution and give one advantage and disadvantage of the isocratic elution. 5M 2\) Write note on paper electrophoresis. Give any two applications of paper electrophoresis 5M 3\) Explain the term headspace analysis. Give its applications. Enlist carrier gases used in gas chromatography. 5M 4\) Write note on stationary and mobile phase employed in affinity chromatography 5M 5\) Enlist the bulk property receptor used in HPLC. Discuss one of them in detail. 5M