Analytical Techniques PDF
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This document is a presentation or lecture on various analytical techniques and their application in scientific fields, like clinical chemistry. It covers topics like spectrophotometry, fluorometry, electrochemical methods, chromatography, mass spectrometry, and more. The document details the components, principles, limitations, and clinical applications of these techniques.
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ANALYTICAL TECHNIQUES TABLE OF Spectrophotometry CONTENT Fluorometry Electrochemistry Chromatography ANALYTIC TECHNIQUES AND INSTRUMENTATION SPECTROPHOTOMETRIC INSTRUMENTS BEER’S LAW TRANSMITTANCE ABSORBANCE EXAMPLE ² ² EXAMPLE ...
ANALYTICAL TECHNIQUES TABLE OF Spectrophotometry CONTENT Fluorometry Electrochemistry Chromatography ANALYTIC TECHNIQUES AND INSTRUMENTATION SPECTROPHOTOMETRIC INSTRUMENTS BEER’S LAW TRANSMITTANCE ABSORBANCE EXAMPLE ² ² EXAMPLE COMPONENTS OF A SPECTROPHOTOMETER COMPONENTS OF A SPECTROPHOTOMETER COMPONENTS OF A SPECTROPHOTOMETER COMPONENTS OF A SPECTROPHOTOMETER COMPONENTS OF A SPECTROPHOTOMETER COMPONENTS OF A SPECTROPHOTOMETER COMPONENTS OF A SPECTROPHOTOMETER LIMITATIONS OF SPECTROPHOTOMETER CLINICAL APPLICATIONS OF SPECTROPHOTOMETER ATOMIC ABSORPTION SPECTROPHOTOMETRY ATOMIC ABSORPTION SPECTROPHOTOMETRY COMPONENTS COMPONENTS LIMITATIONS CLINICAL APPLICATION CLINICAL APPLICATION CLINICAL APPLICATION FLUOROMETRY FLUOROMETER PRINCIPLE LIMITATION APPLICATIONS OF FLUORIMETRY APPLICATIONS OF FLUORIMETRY COMPONENTS COMPONENTS TEST USING FLUOROMETER Protein dynamics Quantitative analysis Cell membrane studies Enzyme kinetics Carbon nanostructures Characterization of fluorescent probes Thermal stability measurements ELECTROCHEMISTRY ELECTROCHEMISTRY PRINCIPLES CLINICAL APPLICATIONS ELECTROPHORESIS PRINCIPLE CLINICAL APPLICATION OSMOMETRY π PARTS OF AN OSMOMETER: LIMITATIONS CLINICAL APPLICATION CHROMATOGRAPHY CHROMATOGRAPHY MODES OF SEPARATION ADSORPTION MODES OF SEPARATION PARTITION MODES OF SEPARATION STERIC EXCLUSION MODES OF SEPARATION GEL FILTRATION AND GEL PERMEATION MODES OF SEPARATION ION-EXCHANGE MODES OF SEPARATION THIN-LAYER (TLC) LIMITATIONS OF CHROMATOGRAPHY CLINICAL APPLICATIONS HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY SAMPLE INJECTORS DETECTORS RECORDERS A B C GAS CHROMATOGRAPHY MASS SPECTROMETRY MASS SPECTROMETRY PRINCIPLE SAMPLE INTRODUCTION AND IONIZATION ELECTRON IONIZATION ATMOSPERIC PRESSURE IONIZATION ELECTROSPRAY IONIZATION ATMOSPERIC PRESSURE CHEMICAL IONIZATION MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETER ANALYZER QUADRUPOLE ION TRAP TANDEM MASS SPECTROMETRY HIGH-RESOLUTION MS Figure 4.46 (A) Pictorial representation of the full width at half maximum (FWHM) definition of mass resolution. (B) Diagram of the principle of time-of-flight (TOF) mass spectrometers. (C) Diagram of Orbitrap mass spectrometry. (A) Reprinted with permission from Thermo Fisher Scientific. (B) © Jones & Bartlett Learning, (C) Reprinted with permission from Thermo Fisher Scientific. DETECTORS THANK YOU
