Biol 1002 Laboratory Quiz 1 PDF

Biol 1002 laboratory Selected material for Quiz 1 – Remember this is not all the material. Quizzes may contain multiple choice, drawings, short answers, and/or brief descriptions. Know definitions. Lab 1 Graduated pipettes are used for accurate measurement of liquid volumes. Most often, they are us...

Biol 1002 laboratory Selected material for Quiz 1 – Remember this is not all the material. Quizzes may contain multiple choice, drawings, short answers, and/or brief descriptions. Know definitions. Lab 1 Graduated pipettes are used for accurate measurement of liquid volumes. Most often, they are used for measuring volumes between 1 ml and 10 ml. Larger volumes require the use of graduated cylinders, whereas smaller volumes require the use of serological or micropipettes (Fig.1). - Know what a graduated cylinder is. - Know what a serological pipette is. - Know what a micropipette is. - Be able to explain which pipette to use for which volume and why. - Be able to calculate dilutions. 1 of 5 Serial Dilution - Be able to explain why you needed serial dilutions. - Be able to explain what the numbers of yeast colonies on the agar plates meant. - Be able to calculate dilutions. - Be able to calculate averages (A) and the standard deviations (δ). Example question: You want to make the following buffer: 2% Milk, 200 mM Tris buffer and 5 mM EDTA in a final volume of 500 mL. Your stock solutions are 20% Milk, 1M Tris buffer and 1M EDTA. The solvent is distilled water. How much volume of each stock solution will you add to make the desired buffer? 2 of 5 Lab 2 An understanding of biological processes on the molecular level first required an understanding of the kinds of molecules that make up biological structures. Know the major types of molecules and their basic building blocks: 1. Carbohydrates 2. Proteins => Amino Acids 3. Lipids => Glycerin backbone, Fatty Acids, 4. Nucleic Acids => Nucleotides (DNA = A,T,C,G) (RNA= A,U,C,G) - Be able to recognize the structure and structural elements of these molecules. - Be able to write the general formula for at least the carbohydrates. Know the tests for the different molecules: Benedicts Test => Reducing sugars Iodine Test => Starch Biuret Test => Proteins Nucleic Acids: DNA => Dische’s Test RNA => Orcinol Test Sudan III => Lipids - Be able to explain what each regent is doing and thus testing for. - For example, be able to recall what color changes the reagents caused and what that indicated. - Be able to explain what you would do to identify an unknown substance. Structures of macromolecules: 3 of 5 Examples: - Be able to describe the difference between a phospholipid and a triglyceride. - Be able to recognize a lipid and its components. - Be able to recognize different carbohydrate examples. - Be able to recognize a nucleic acid molecule and its components. - Be able to recognize what a nucleotide is. 4 of 5 Example question: Describe the Biuret test: a. What is/are the positive results of this test? b. What is/are the negative results of this test? c. What is the chemical mechanism behind this test? d. What conclusions can you draw from the Biuret Test? 5 of 5