Medical Microbiology Lab 1 PDF 2015

Document Details

GoldNobility2469

Uploaded by GoldNobility2469

National University of Science and Technology

2015

MSc. Farah Kadhum Githeeth,MSc. Russul Ismaeil

Tags

medical microbiology laboratory safety microscope biology

Summary

This document is a laboratory manual for Medical Microbiology Lab 1 at the National University for Science and Technology in 2015. It covers laboratory safety procedures, microscope parts and usage, and introductory concepts for medical microbiology.

Full Transcript

‫الجامعة الوطنية للعلوم والتكنولوجيا‬ ‫‪Medical Microbiology‬‬ ‫‪Lab 1‬‬ ‫م م فرح كاظم غثيث‬ ‫م م رسل اسماعيل‬ ‫‪1‬‬ Medical Microbiology Lab1 1-Microbiology Laboratory Safety and Rules 2- Microscope MSc. Farah Kadhum Gi...

‫الجامعة الوطنية للعلوم والتكنولوجيا‬ ‫‪Medical Microbiology‬‬ ‫‪Lab 1‬‬ ‫م م فرح كاظم غثيث‬ ‫م م رسل اسماعيل‬ ‫‪1‬‬ Medical Microbiology Lab1 1-Microbiology Laboratory Safety and Rules 2- Microscope MSc. Farah Kadhum Githeeth MSc. Russul Ismaeil Microbiological procedures including  Do not bring food and drink, into the lab.  Do not invite friends into the lab.  Keep pencils, fingers, out of your mouth, ears, eyes, nose.  Wear protective clothing at all times.  Shoes are always required  Open-toed sandals are not allowed.  Do not take any cultures out of the lab for any reason.  Keep nonessential books and clothing far away from your work area. 3  Wash your hands with soap and water for 15 seconds before and after you work in the lab, or if you leave the lab for any reason.  Do not mouth pipette ever!!  Be careful near Bunsen burners.  Turn off burners when not in use.  Beware of the chemicals and reagents used in biochemical tests.  Report any accidents (spills, broken glass, cuts, or injuries) to removed.  Dispose of contaminated or used materials quickly.  Label all of your tubes and plate.  Place all cultures for incubation exactly. 4 Microscope 5 Basic Microscope Parts Base 1. Light source.2 Diaphragm.3 Stage.4 Stage clips.5 Objectives.6 Nosepiece.7 Arm.8 Fine Focus.9 Body Tube.10 Course Focus.11 Eyepiece.12 6 the Arm and Base Supports Arm Used Bottom Base to part carry theofbody the themicroscope. tube. microscope often shaped like a horseshoe. Arm Supports the body tube. Used to carry the microscope. Base Bottom part of the microscope often shaped like a horseshoe. 7 Stage and Stage Clips Stage Place where the object you are looking at is placed (Specimen). Stage Clips. Holds down the slide on the stage. 8 Eyepiece (Ocular Lens) and Body Tube Eyepiece The lens you look through that magnifies the specimen. Body Tube The hollow tube through which light passes. It holds the lenses a part. 9 Objective (Low Power/High Power Low Power Objective Magnifies the specimen at a lower power High Power Objective Magnifies the specimen at a Higher power 10 Diaphragm and Light Source Diaphragm Changes the amount of light reaching the objective lenses. Located under the Stage Light Source Located beneath the Stage and Diaphragm. Sends light towards the hole in the stage. 11 Coarse adjustment. It used to cheive an approximate focuisng Fine adjustment It used to being the object into perfect focus 12

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