Lab (1) Tools, Instruments & Equipment PDF

Lab (1) Tools, Instruments & Equipment Loop: Used for transfer of bacterial cells from medium to another. sterilized by the flame of burner after and before using. Pipette: Used for transfer of cultured and uncultured broth from tube or flask to other, and placed in the can. Steriliz...

Lab (1) Tools, Instruments & Equipment Loop: Used for transfer of bacterial cells from medium to another. sterilized by the flame of burner after and before using. Pipette: Used for transfer of cultured and uncultured broth from tube or flask to other, and placed in the can. Sterilized in Autoclave (with can). Spreader: Used for spreading bacterial cells on the surface of solid medium in Petri plate. Before using placed in alcohol and then sterilized by flame of burner (before using). Swab: Used for swabbing the bacterial cells on the surface of solid medium in Petri plate, must be placed in test tube and sterilized by Autoclave (with test tube), must be disinfected before disposal. Needle: Used for transfer bacterial cells to a solid medium or semi- solid medium by stabbing, sterilized by the flame of burner after and before using. Slide: Used for the examination. Placed on microscope stage. Cover- slips: Used to keep solid specimens pressed flat and liquid samples shaped into a flat layer of even thickness. Placed on the slide, the sample will be between the cover and the slide. Test tube: Test tubes are very important laboratory tools in the biological, chemical, and medical sciences. In general, they are used to mix chemicals, hold liquids, heat liquids, grow bacteria, and collect or store bodily fluids, like blood and semen. Petri- dish: Used for place the solid medium in it. Glass Petri – plate used for many times & sterilized Plastic Plates use for one time. Flask: Used for place cultured and uncultured broth in it. Sterilized after plugs with cotton by Autoclave Beaker: Used for graduate the volume of liquid. Sterilized by oven. Graduated cylinder: Used for graduate the volume of liquid. Sterilized by oven. Washing bottle: Used to fill with liquids (specially distilled water) for washing and homogenizing the glass wares and washing the slide during the staining. Don't need to sterilization. Burner: May be gaseous or alcoholic, used for sterilize the loop, needle and other metal tools by the flame (Dry heat Sterilization). PH Paper: Used to know the pH of the medium or any liquid. Autoclave: Sterilization by autoclave named wet heat Sterilization. Used for sterilize the culture media and some of metal tools and glass wares. ** The temperature = 121Co ** Pressure = 1 atm. (15 pound/ Inch2) ** Time = 10-30 min. 10 min. (for media with sugar) 15 min. (for uncultured media) 30 min. (for cultured media, contaminated tools & glass wares) Oven: The Sterilization is dry heat Sterilization, used for sterilize the glass wares and some of metal tools. ** Temp. = 180 Co ** Time = one hour and half Incubator: Used for the availability of suitable temperature for growth of microorganisms by place the culture media in it. For example: pathogenic bacteria grow in optimal temperature 37 Co for 18-24 hours (the optimum 24 hours). Refrigerator: Used to place the sterilized media and broth when not used to avoid the contamination and also used to preserve the bacteria cultures for long time by preventing the growth in 4 Co