DNA-Binding Motifs and Engineered Nucleases Quiz PDF

11/23/23, 12:02 PM DNA-Binding Motifs and Engineered Nucleases Quiz DNA-Binding Motifs and Engineered Nucleases Quiz Your Name: 1. Which type of promoters use more regulators: eukaryotic or bacterial? A. Both eukaryotic and bacterial promoters use the same number of regulators B. It depends on the...

11/23/23, 12:02 PM DNA-Binding Motifs and Engineered Nucleases Quiz DNA-Binding Motifs and Engineered Nucleases Quiz Your Name: 1. Which type of promoters use more regulators: eukaryotic or bacterial? A. Both eukaryotic and bacterial promoters use the same number of regulators B. It depends on the specific gene being regulated C. Bacterial promoters D. Eukaryotic promoters 2. What type of proteins affect the regulation and transcription initiation of a gene by binding to a regulatory sequence near or within the gene and interacting with RNA polymerase and/or other transcription factors? A. Ribosomal proteins B. DNA polymerase enzymes C. Transcription Factors (TF) D. Histones 3. Which motif is NOT mentioned in the key terms provided? A. Zinc finger motif B. Basic leucine zipper motif C. Homeodomain motif D. ATP-binding motif 4. Where can regulator-binding sites be located in eukaryotic genes? A. Upstream and downstream from the promoter B. Only upstream from the promoter C. Only within the coding sequence of the gene itself D. Exclusively within the promoter region 5. How are bacterial promoters usually regulated? A. By a combination of RNA polymerase and ribosomal proteins B. By only one or two regulatory proteins C. They are not regulated D. By multiple transcription factors 6. Which type of genes usually have numerous regulator-binding sites and can span a large region? A. Eukaryotic genes, especially those of multicellular organisms B. Prokaryotic genes C. Viral genes D. Bacterial genes 7. What is the function of a recognition helix in a transcription factor? https://quizgecko.com/quiz/230150/print 1/6 11/23/23, 12:02 PM DNA-Binding Motifs and Engineered Nucleases Quiz A. It interacts with RNA polymerase B. It stabilizes the DNA double helix structure C. It catalyzes the transcription process D. It binds to a regulatory sequence near or within the gene 8. Which protein affects the regulation and transcription initiation of a gene by binding to a regulatory sequence near or within the gene and interacting with RNA polymerase and/or other transcription factors? A. Transcription factor B. DNA polymerase C. Histone D. Ribosomal protein 9. Which type of promoters are usually near, or overlap, the coding region? A. Viral promoters B. Bacterial promoters C. Fungal promoters D. Eukaryotic promoters 10. What is the main difference between the regulation of bacterial and eukaryotic promoters? A. Eukaryotic promoters are exclusively regulated by RNA polymerase B. Bacterial promoters are regulated at the post-transcriptional level C. Eukaryotic promoters use more regulators than bacterial ones D. Bacterial promoters have more transcription factor binding sites 11. What are TALE and TALEN examples of? A. Transcription factors B. Restriction enzymes C. Ribosomal proteins D. DNA polymerases 12. What is the role of a basic helix-loop-helix motif? A. It catalyzes the transcription process B. It stabilizes the DNA double helix structure C. It interacts with RNA polymerase D. It binds to a regulatory sequence near or within the gene 13. Which motif is primarily used for dimerization? A. Basic helix-loop-helix B. Helix-turn-helix C. Basic leucine zipper D. Homeodomain 14. Which TF motif is associated with development or cell cycle activity? A. Homeodomain B. Helix-turn-helix https://quizgecko.com/quiz/230150/print 2/6 11/23/23, 12:02 PM DNA-Binding Motifs and Engineered Nucleases Quiz C. Basic leucine zipper D. Basic helix-loop-helix 15. Which motif is linked to cancer and includes proteins like c-Myc and HIF-1? A. Zinc finger B. Basic leucine zipper C. Homeodomain D. Basic helix-loop-helix 16. What is the function of proteins with zinc finger motifs? A. DNA replication B. RNA translation C. DNA recognition D. Protein synthesis 17. What stabilizes the elongated loop in the zinc finger motif? A. Mg2+ ions B. Fe2+ ions C. Zn2+ ions D. Cu2+ ions 18. Which engineered nuclease is designed using zinc fingers joined to a FokI-derived DNA cleavage domain? A. Zinc-finger nucleases (ZFNs) B. Meganucleases C. Transcription activator-like effector nucleases (TALENs) D. CRISPR-Cas9 19. What is the length of the homeodomain motif in amino acids? A. 60 B. 20 C. 30 D. 40 20. What is the length of the basic leucine zipper motif in amino acids? A. 20-30 B. 30-40 C. 60-80 D. 50-60 21. Which type of zinc finger motifs have been designed for gene editing due to their weak single zinc finger interactions? A. Type II B. Type III C. Type IV D. Type I https://quizgecko.com/quiz/230150/print 3/6 11/23/23, 12:02 PM DNA-Binding Motifs and Engineered Nucleases Quiz 22. What distinguishes Type II zinc finger motifs from Type I? A. Combination with helix-turn-helix motif B. Length of the motif C. Binding to DNA D. Presence of Zn2+ ions 23. Which engineered nuclease is designed using TALEs fused to FokI nucleases? A. CRISPR-Cas9 B. Zinc-finger nucleases (ZFNs) C. Meganucleases D. Transcription activator-like effector nucleases (TALENs) 24. What is the length of the basic helix-loop-helix motif in amino acids? A. 60-70 B. 40-50 C. 30-40 D. 50-60 25. What distinguishes TALEs from TALENs? A. The presence of a homeodomain motif in TALENs B. The ability to form dimers in TALEs but not in TALENs C. The presence of a FokI nuclease domain in TALENs D. The ability to bind to DNA in TALEs but not in TALENs 26. What is the primary function of the basic helix-loop-helix motif? A. Dimerization of proteins B. Binding to zinc ions C. Regulation of gene expression during development D. Interaction with RNA polymerase 27. Where can regulator-binding sites be located in eukaryotic genes? A. Only within the coding sequence of the gene B. Both upstream and downstream from the promoter C. Only upstream from the promoter D. Exclusively within the promoter region 28. What is the main difference between the regulation of bacterial and eukaryotic promoters? A. Extent of regulator-binding sites B. Number of regulatory proteins involved C. Type of transcription factors involved D. Location of the promoter relative to the coding region 29. What is the function of the FokI-derived DNA cleavage domain in zinc-finger nucleases (ZFNs)? A. It stabilizes the zinc finger motif https://quizgecko.com/quiz/230150/print 4/6 11/23/23, 12:02 PM DNA-Binding Motifs and Engineered Nucleases Quiz B. It facilitates the cleavage of DNA at specific target sites C. It enhances the binding of zinc fingers to DNA D. It regulates the dimerization of zinc fingers 30. Which motif is NOT mentioned in the classification of Transcription Factors (TFs) based on specific conserved motifs? A. Helix-turn-helix B. Homeodomain C. Zinc finger D. ATP-binding motif 31. What is the length of the zinc finger motif in amino acids? A. 30 B. 50 C. 40 D. 60 32. Which type of zinc finger interactions are weak? A. Type I zinc finger interactions B. Single zinc finger interactions C. Type II zinc finger interactions D. Multiple zinc finger interactions 33. What is the primary role of the basic leucine zipper motif? A. RNA packaging B. DNA recognition C. Transcriptional activation D. Dimerization 34. Which engineered nucleases are designed using TALEs fused to FokI nucleases? A. Transcription activator-like effector nucleases (TALENs) B. Meganucleases C. CRISPR-Cas9 D. Zinc-finger nucleases (ZFNs) 35. What is the function of the helix-turn-helix (h-t-h) motif in regulatory proteins? A. DNA binding B. Transcriptional activation C. Dimer formation D. RNA packaging 36. Which TF motif is associated with body pattern development in various organisms, including humans? A. Helix-loop-helix B. Leucine zipper C. Zinc finger https://quizgecko.com/quiz/230150/print 5/6 11/23/23, 12:02 PM DNA-Binding Motifs and Engineered Nucleases Quiz D. Homeodomain 37. What stabilizes the elongated loop in the zinc finger motif? A. Protein kinase B. DNA C. Zn2+ ions D. RNA 38. What is the length of the homeodomain motif in amino acids? A. 40 B. 30 C. 60 D. 50 39. Which motif is primarily used for DNA binding, dimer formation, and is often associated with development or cell cycle activity? A. Basic helix-loop-helix (b-hlh) B. Leucine zipper C. Helix-turn-helix D. Zinc finger 40. What distinguishes Type II zinc finger motifs from Type I? A. Type II is shorter in length B. Type II is only found in eukaryotic proteins C. Type II combines the Zn2+-binding motif with the helix-turn-helix motif D. Type II has stronger interactions with DNA 41. Explain the function and structure of the zinc finger motif in DNA-binding proteins. 42. Describe the role and significance of the homeodomain motif in transcription factors. 43. Discuss the structure and function of the basic helix-loop-helix motif in DNA-binding proteins. 44. Explain the classification of transcription factors based on specific conserved motifs, and provide an example for each type of motif mentioned. 45. Discuss the role of TALENs in gene editing and the structural components involved in their function. https://quizgecko.com/quiz/230150/print 6/6