MTAP 1 - Assessment 2 (HTMLBE-LabMan).pdf

HTMLBE-LabMan A basic acridine fluorochrome which permits Assessment 2 discrimination between dead and living cells, giving green fluorescence to D...

HTMLBE-LabMan A basic acridine fluorochrome which permits Assessment 2 discrimination between dead and living cells, giving green fluorescence to DNA and red fluorescence for Compulsory HIV Testing is considered unlawful by RA RNA. 8504. Which of the following is an exception to the Acridine orange prohibition on … testing? Methyl green When complying with the provisions of RA Pyronine 7170. Acridine red 3B Determination of HIV status pursuant to the Family code of the Philippines. Composed a basic fuchsin dissolved in 70% alcohol and When a person is charged with any crimes paraldehyde is: punishable under RA 8335. Mallory's fuchsin When a person is charged with any crimes Feulgen's reagent punishable under RA 3815. Gomori's aldehyde fuchsin Schiff’s reagent Sections of brain that tend to wash off the slide during staining can be adhered to slides by coating the sections A metachromatic dye formed when methylene blue is / slides with: heated in fixed alkali or alkali carbonate: Paraffin Cresyl violet Celloidin Methylene violet Carbowax Toluidine blue Polyethylene glycol Crystal violet Poly-l-lysine (quizlet) The "stain" for electron microscopy usually contain: Which of the following knife profiles is recommended for Cyclic hydrocarbons the majority of paraffin microtomy? Brightly colored dyes Tool edge Natural dyestuffs Wedge Heavy metals Biconcave Fluorescent compounds Plane concave Which of the following principles of collaborative Microscopic examination of an H&E-stained section management emphasizes the importance of employees reveals the presence of extraneous tumor cells. This is making their own decisions without being manipulated or most likely caused by: controlled? Sections taken immediately after "rough facing" Trust of block. Understanding Flotation bath not cleaned between blocks. Independence Albumin spread on the slide with a finger. Solutions Excess section adhesive on the slide. A heterogenous group of yellow-brown pigments, often Which of the following stains would you choose to study found in cardiac muscle and liver cells in increasing the ground substance carbohydrates? amounts with age or debilitated states is known as: Alcian blue-PAS Lipofuscins Hematoxylin and eosin Melanins Orcein Van Gleson Porphyrins Acid fuchsin aniline blue Hemoglobins Da Fano’s cobalt nitrate The term "argentaffin" literally refers to a reaction Normal elastic fibers: wherein cells have the ability to reduce a salt of: Stain with basic dyes. Copper Stain with acid dyes only after treatment with Silver chromic acid. Chromium Stain with acid dyes. Lithium Are removed from tissue by osmic acid. Are removed from tissues by dilute acids. A good fixative is one that: Enhances putrefaction of tissue components. Does not harden tissues quickly. Makes tissue more permeable to fluids. Preserves tissues in an un-life-like manner. CARBON, BUTTERSCOTCH Helly's fluid contains all of the following EXCEPT: An excellent stain for elastin fibers (used in Sodium suplhate Taenzer-Unna method) and is especially recommended Mercuric chloride in dermatological studies due to its ability to demonstrate Glacial acetic acid the finest and most delicate fibers of the skin: Potassium dichromate Night blue Orcein Chromaffin granules are best demonstrated following Carmine fixation in: Celestine blue 10% BNF Zenker solution The pigment that is formed following a reaction of B5 solution ferrous iron with potassium ferricyanide is known as: Orth’s fluid Turnbull blue Nile red The most difficult step to remedy in the preparation of a Prussian blue high quality microscopic slide of tissue is: Alizarin red Poor staining Incorrect section placement Following immunoperoxidase staining, it is observed that Poor fixation the positive control stains well, but there is no staining of Improper sectioning a specimen expected to be positive. The most likely cause is that the: Uric acid crystals are preserved only when the tissue is Staining steps were performed in the wrong fixed in: order. Acetone fixed tissue Primary antibody was diluted by excess buffer or Alcohol fixed tissue non-immune serum. Zenker’s fixed tissue Specimen was improperly counterstained. Formalin fixed tissue Specimen was improperly fixed. It would be most unusual to demonstrate enzymes on: With regard to the avidin-biotin-complex (ABC) Orth's fluid immunoperoxidase procedure: Zamboni solution The ABC binds to biotin-labeled secondary Absolute alcohol antibody. Neutral buffered formalin The bridging antiserum is added in excess. The primary antiserum is avidin-labeled. Prior to decision making, a leader consults his / her No conjugation steps are involved. members. The decision will be based on the majority. What leadership style is being shown? In hiring, what do you call the oral process of confirming Democratic the information in the applicant's information? Authoritative Speech Laissez-Faire Introductory Message Charismatic Interview Confirming speech Chromaffin granules are best demonstrated following fixation in: Fixative used for the preservation of some enzymes is: Orth’s fluid Acetone B5 solution Mercuric chloride Zenker solution Cold buffered saline 10% NBF Bouin fluid B5 fixative contains all of the following EXCEPT: In immunoperoxidase staining of formalin-fixed tissue, Glacial acetic acid enzyme pretreatment of antigens: Mercuric chloride Is needed to demonstrate all antigens. 37 to 40% formaldehyde Has precise end-points. Sodium acetate Enhances background staining. Reactivates antigens and enhances primary staining. CARBON, BUTTERSCOTCH Soft, mushy appearing paraffin sections can be caused It is responsible for preventing, investing and combating by: any dangerous drugs, controlled precursors and Paraffin is too warm. essential chemicals in the Philippines. Inadequate processing. Dangerous Drug Board The type of tissue process. Phil. Drug Enforcement Agency Over dehydration National Bureau of Investigation Phil. National Police When frozen sections stick to the anti-roll plate of the cryostat, the plate is: Refers to the selective removal of excess stain from the Not parallel to the knife edge. tissue during regressive staining in order that a specific Too far below the knife edge. substance may be stained distinctly from the Too far above the knife edge. surrounding tissues. Too warm Intravital staining Supravital staining As the block face is trimmed, the knife digs into the Counterstaining tissue and gouges out a chunk. The most probable Decolorization cause is that the: Block is loose in the chunk. A diamond knife should not be used to cut tissue Block is too cool. containing: Cutting stroke is too rapid. Lipid Knife is dull. RBC Amyloid Tissue can’t be fixed in the microwave oven because of: Calcium lonizing radiation Rupture of hydrogen bonds. Layered structures such as cyst wall and gall bladder Rapid molecular movement. should be embedded: Reaction between protein molecules. On the end Parallel Which of the following fixatives is recommended for lipid Lengthwise histochemistry? On edge Formalin saline Calcium formalin The instrumentation QC manual states that floatation Zenker solution baths must be emptied and thoroughly cleaned on Acetone Fridays. The bath may be refilled and additional adhesive may be added as needed during the week. After completion of decalcification, the specimen are This procedure: stained with: May lead to bacterial contamination of Washed in 70% alcohol. microscopic sections. Placed in acetone. Will probably increase section loss during Washed in water. staining. Placed in fixative. May be considered satisfactory. NOTA Should be changed to read "emptied and cleaned twice a week”. The "thin" sections for EM are stained with: Uranyl acetate and lead citrate An aqueous mounting medium was prepared 3 months Hematoxylin and eosin ago and stored in a 4°C refrigerator. Mold is now Fluorescein and rhodamine observed in the medium. To prevent this in the future, Toluidine blue when preparing the medium, one should: Increase the amount of glycerol. The recommended fixative for tissue suspected of Boil the solution. containing spirochetes is: Be sure to add thymol. Zenker solution Filter the newly made solution. Helly solution 10% NBF One cause of sections being lifted from the knife on the Bouin solution up stroke is that the: Room or paraffin is too cold. Specimen is too hard. Knife is too sharp. Knife tilt is incorrect. CARBON, BUTTERSCOTCH Which of the following types of operating budget When the MedTech said to the patient, "Ma'am I will addresses only changes such as new equipment, new insert the needle," and then the patient nod, is an positions, and new programs and is based on the example of what consent? assumption that existing operations are essential to the Explicit consent process? Implied consent Fixed Oral consent Program Written consent Appropriation Incremental The process of calcium removal in bone before paraffin Flexible infiltration is called: Dehydration Fixative mixtures use glacial acetic acid in combination Decalcification with other primary fixative agents in order to: Staining Decrease post-mortem alterations. Fixation Counteract the shrinkage effect. Increase the speed of fixation. The temperature (in °C) used in a cryostat for cutting Prevent bacterial contamination. frozen sections is: 37°C When tissue is to be fixed for enzyme histochemical 10°C studies, fixation: 56°C Prior to sectioning increases enzyme solubility. -20°C Prior to sectioning reduces enzyme diffusion. The best substitute for ethyl alcohol as a dehydrating Must always follow cryostat sectioning. agent is: Should be done using gum sucrose solution. Ether Isopropyl alcohol A good substitute for hematoxylin stain is: Xylol Azure A Methanol Safranin Celestine blue B Expenses for an autopsy are shouldered by the: Methyl violet Relatives of the deceased. Hospital Laboratory reports must be given by the Medical Medical Technologist Technology only to: Pathologist Patient's family Medical director Masson-Fontana method is a stain for: Requesting physician Phospholipid Patient Melanin Connective tissue The formalin pigments can be removed from the tissue Muscle and bone by soaking it in: Nitric acid The formalin pigments can be removed from the tissue Acid alcohol by soaking it in: Xylene Nitric acid Saturated alcoholic picric acid Acid alcohol Xylene If the water used in washing of the Mayer's hematoxylin Saturated alcoholic picric acid stain is not sufficiently alkaline, this solution should be added for alkalinization: An autopsy to be most informative and helpful should be Sodium thiosulfate done within: Lithium carbonate 36 hours Ammonium chloride 48 hours Sodium hydroxide 72 hours 24 hours Smears and cell block of urine for cytological examination will show desquamated cells from the lining The only substance used in histopathology as both a epithelium of the urinary tract which are: fixative and a dye is: Transitional cells Picric acid Mucous cells Acetic acid Squamous cells Osmic acid Columnar cells Oxalic acid CARBON, BUTTERSCOTCH Which of the following is true about the requirements for Which of the following circumstances will result in a a clinical laboratory to operate? penalty according to RA 5527? 1. The laboratory shall conform to the required space 1. A registered medical technologist working in an for the conduct of its activities. unlicensed clinical laboratory. 2. The clinical laboratory shall participate in an EQAP 2. A medical technologist from another country, doing administration. consultation for a university. 3. Every clinical laboratory shall be headed and 3. A registered medical technologist working in two managed by a pathologist. clinical laboratories. 4. Clinical laboratory shall have a written policies and 4. A duly registered medical technologist, after a procedures. warning, fails to display his / her certificate of 1 only registration. 1 and 2 I, II, III, IV 1, 2, and 3 I, III, IV 1, 2, 3, and 4 II, IV I, IV In the papanicolaou method, the counterstain used has a high: The most widely used fixative for electron microscopy. Salt content 10% formalin Alcohol content Osmium tetroxide Acid content Lead nitrate formalin Water content Orth’s fluid A relatively simple stain of myelin is: One characteristics of 2 to 3% glutaraldehyde is that it: Best Carmine Chemically reacts with lipids. Oil Red O Allows many histochemical reactions on OG fixed specimens. Lugol Fast Blue Only partially preserves proteins. Penetrates rapidly. Congo red staining method is used to demonstrate: Mucin To increase the amount of light when viewing a Amyloid microscopic section, an adjustment should be made in Fat the: Glycogen Substage condenser Iris diaphragm Helly's fluid is the same as Zenker's fluid except that it Objective contains formalin instead of: Numerical aperture Glacial acetic acid Hydrochloric acid When it is known prior to fixation that a distinction must Ammonium hydroxide be made between collagen and muscle, the preferred Sulfuric acid fixative is: Absolute alcohol The chairman and two members of the Board of Medical Bouin fluid Technology have a term of: NBF Two years Orth fluid One year Three years Which of the following fixatives requires washing with Four years water before processing? 10% formalin Ribbons of tissue should be floated on water maintaining Zenker a temperature in this °C. Carnoy 20-25 Bouin 60-65 40-45 An H&E stained section is microscopically evaluated for 50-55 quality. Which of the following is sufficient criterion for rejection of the slide? An example of microanatomical fixative is: Well defined chromatin. 10% formol saline Bright pink-red erythrocytes. Flemming’s fluid Crisp blue nuclei. Newcomer’s fluid Pale pink to gray collagen. Carnoy’s fluid CARBON, BUTTERSCOTCH "As I enter the practice of Medical Technology, I shall ___ the responsibilities inherent to being a professional.” Accept Receive Do Consent "I shall use ___ means of competition for professional employment or services." Acceptable Practical Reasonable Sound "I hereby subscribe and pledge to conduct myself at all times in a manner befitting the ___ of my profession.” Respect Dignity Pride Piousness “I shall report any ___ of these principles of professional conduct to the authorities..." Infractions Omissions Infarctions Subtractions "I shall accept employment from more than one employer only when there is no ___.” Conflict of interest Disability Possibility Lack of interest CARBON, BUTTERSCOTCH