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### Buffers **3. Citrate Buffer** * **Reagents required:** * Citric acid: Dissolve 2.101 gm of citric acid in 100ml distilled water. * Sodium Citrate solution 0.1M: Dissolved 2.941 gm of sodium citrate in 100ml distilled water. * **Procedure:** * 46.5ml of citric acid with 3.5ml of...

### Buffers **3. Citrate Buffer** * **Reagents required:** * Citric acid: Dissolve 2.101 gm of citric acid in 100ml distilled water. * Sodium Citrate solution 0.1M: Dissolved 2.941 gm of sodium citrate in 100ml distilled water. * **Procedure:** * 46.5ml of citric acid with 3.5ml of sodium citrate solution and upto 100 ml with distilled water. * It corresponds to 0.1M Citrate buffer and standardised with PH meter and measures the PH of the Prepared solution. This gives Citrate buffer at PH 2.5. * **Result:** Citrate buffer was prepared and the PH observed was 4.8 which was adjusted to 2.5 using IN Hal and 5N NaOH. **4. Carbonate - Bicarbonate Buffer** * **Requirement:** Sodium Carbonate Solution 0.2M: Dissolve 2.12gm of Anhydrous sodium carbonate in 100ml Distilled water sodium