Questions and Answers
What is the recommended duration of formalin fixation?
2 to 6 hours
What is the ideal volume of fixative in relation to tissue volume for maximum effectiveness?
20 times the tissue volume
What are the characteristics of a good fixative based on the text?
Cheap, stable, safe to handle
What effect do fixatives have on tissues based on the text?
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What should be done if autopsy materials cannot be fixed immediately?
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What happens if fixation is prolonged according to the text?
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What is the main goal of fixation in histotechnology?
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Which type of fixation involves the fixing agent becoming part of the tissue by forming cross links?
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What is the ideal pH range for fixation in histotechnology?
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In what temperature range are tissues processed for electron microscopy fixation?
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What effect does hypertonic fixative have on cells during fixation?
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What is the recommended concentration of glutaraldehyde for immuno electron microscopy fixation?
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What is the purpose of placing a primarily fixed tissue in a 2.5 - 3% potassium dichromate solution for 24 hours?
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Which solution is used to wash out excess chromates from tissues fixed in Kelly’s, Zenker’s, and Flemming solutions?
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What factor enhances the process of fixation?
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How does cold temperature affect fixation?
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Which type of fixative is known as a microanatomical fixative?
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Why is washing out tap water used after fixing tissues?
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