Microbial Taxonomy and Bacterial Genetics Quiz

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22 Questions

Which staining method is commonly used for Helicobacter pylori?

Warthin Starry

What is the staining method typically used for Legionella?

Machielvello

Which staining method is NOT associated with Gram Stain?

Warthin Starry

What technique is primarily used to estimate the number of bacteria present by Total Plate Count?

Total Plate Count method

Which staining method is typically used for Spirochetes?

Dienne's

What type of stain is commonly associated with Mycoplasma?

Dieterle's Stain

What is the order of the classification of microbes according to Carl Von Linne?

Kingdom → Phylum → Class → Order → Family → Genus → Species → Subspecies

Who discovered the helical structure of DNA through X-RAY studies?

Rosalind Franklin

What are the nucleotides in DNA composed of?

Phosphate, Deoxyribose, Bases

What is the role of DNA polymerase in bacterial genetics?

Protein synthesis

Which extrachromosomal element is known for carrying genes related to antimicrobial resistance?

Plasmids

What process involves bacteria taking up naked DNA from dying bacteria?

Transformation

Which type of organism has DNA that is not enclosed in a membrane?

Prokaryotes

What is the main component of the cell wall in Gram-positive bacteria?

Teichoic Acid

Which organism lacks a cell wall?

Mycoplasma

Which staining technique is used to visualize acid-fast bacteria like Mycobacteria?

Ziehl-Neelsen staining

In which type of bacteria is the outer membrane present?

Gram-negative bacteria

Which type of bacteria has a thin peptidoglycan layer in its cell wall?

Gram-negative bacteria

What gives a negative charge to Gram-positive bacteria?

Teichoic Acid

Which bacteria lack organelles and histones?

Prokaryotes

Which staining technique is used for Gram-staining to identify bacterial cell walls?

Crystal Violet staining

Which characteristic differentiates Mycoplasma from other bacteria?

Lack of cell wall structure

Study Notes

Staining Methods

  • Helicobacter pylori is commonly stained using the Warthin-Starry silver stain method.
  • Legionella is typically stained using the Dieterle silver stain method.
  • Acid-fast staining is NOT associated with Gram Stain.

Microbial Quantification

  • Total Plate Count is primarily used to estimate the number of bacteria present.

Microbial Classification

  • Carl Von Linne classified microbes in the order: Kingdom, Phylum, Class, Order, Family, Genus, Species.

DNA Structure and Function

  • James Watson and Francis Crick discovered the helical structure of DNA through X-RAY studies.
  • DNA nucleotides are composed of sugar, phosphate, and nitrogenous bases.
  • DNA polymerase plays a crucial role in bacterial genetics by synthesizing new DNA strands.

Extrachromosomal Elements

  • Plasmids are known for carrying genes related to antimicrobial resistance.

Gene Transfer

  • Transformation involves bacteria taking up naked DNA from dying bacteria.

Cell Wall Characteristics

  • Prokaryotic organisms have DNA that is not enclosed in a membrane.
  • The main component of the cell wall in Gram-positive bacteria is peptidoglycan.
  • Mycoplasma lacks a cell wall.

Staining Techniques

  • Acid-fast staining is used to visualize acid-fast bacteria like Mycobacteria.
  • Gram-staining is used to identify bacterial cell walls by staining peptidoglycan.
  • Crystal violet, iodine, and safranin are used in Gram-staining.

Cell Characteristics

  • Outer membrane is present in Gram-negative bacteria.
  • Gram-negative bacteria have a thin peptidoglycan layer in their cell wall.
  • Teichoic acid gives a negative charge to Gram-positive bacteria.
  • Bacteria lack organelles and histones.
  • Mycoplasma is differentiated from other bacteria by its lack of a cell wall.

Test your knowledge on microbial taxonomy from Kingdom to Species, as well as concepts in bacterial genetics including DNA structure, transcription, translation, and key discoveries by scientists. Explore the genetic makeup and characteristics of bacteria.

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