Which method can be used to introduce plasmids into cultured cells? A) Electroporation B) Sonoporation C) Lentiviral transduction D) Microinjection
Understand the Problem
The question is asking about the various techniques that can be utilized to introduce plasmids into cultured cells. It provides multiple choice options for possible methods.
Answer
Electroporation, sonoporation, lentiviral transduction, and microinjection.
Electroporation, sonoporation, lentiviral transduction, and microinjection can all be used to introduce plasmids into cultured cells.
Answer for screen readers
Electroporation, sonoporation, lentiviral transduction, and microinjection can all be used to introduce plasmids into cultured cells.
More Information
All four methods listed - electroporation, sonoporation, lentiviral transduction, and microinjection - are viable techniques for introducing plasmids into cells. They differ in mechanism and context of use, with some being more suitable for particular types of cells or experimental settings.
Tips
Often, learners might mistakenly assume one method is exclusive or significantly superior for all scenarios. It's important to consider the specific experimental context and cell type.
Sources
- Transfection types, methods and strategies: a technical review - PMC - pmc.ncbi.nlm.nih.gov
- DNA Transfection - an overview | ScienceDirect Topics - sciencedirect.com
- The Power of Lentiviruses in Cell Engineering - BPS Bioscience - bpsbioscience.com
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