What method is primarily used to introduce a DNA fragment into a bacterial plasmid?
Understand the Problem
The question is asking which method is mainly used to insert a DNA fragment into a bacterial plasmid, indicating a focus on molecular biology techniques. The options offered suggest a comparison of different methodologies used in genetic engineering.
Answer
Transformation
The method primarily used to introduce a DNA fragment into a bacterial plasmid is transformation.
Answer for screen readers
The method primarily used to introduce a DNA fragment into a bacterial plasmid is transformation.
More Information
Transformation involves using methods such as heat shock or electroporation to make bacterial cells more permeable and able to take up foreign DNA efficiently. It's a critical process in genetic engineering and molecular cloning.
Tips
Don't confuse bacterial transformation with transfection, which refers to introducing DNA into eukaryotic cells, not bacterial cells.
Sources
- Overview DNA cloning - khanacademy.org
- Bacterial transformation & selection - khanacademy.org
- Bacterial Transformation Workflow | Thermo Fisher Scientific - US - thermofisher.com
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