What is the method for determining hemoglobin (Hb) content in blood, including details about the Sahli method and equipment used?
Understand the Problem
The question is providing detailed steps and principles for determining hemoglobin (Hb) content in blood, focusing particularly on the Sahli method and the apparatus used. It discusses the procedure and the materials involved in the testing process.
Answer
Sahli method uses dilute HCl to convert hemoglobin to acid hematin, matched with a color comparator using Sahli's hemoglobinometer.
The Sahli method converts hemoglobin to acid hematin using dilute HCl. The solution’s color is matched against a comparator to determine Hb concentration, using a Sahli’s hemoglobinometer including a calibrated tube, pipette, dropper, and color comparator.
Answer for screen readers
The Sahli method converts hemoglobin to acid hematin using dilute HCl. The solution’s color is matched against a comparator to determine Hb concentration, using a Sahli’s hemoglobinometer including a calibrated tube, pipette, dropper, and color comparator.
More Information
Sahli's method is simple and widely used, especially in areas with limited resources, due to its reliance on basic visual comparisons rather than expensive equipment.
Tips
Ensure accurate dilution and color matching as human error in visual interpretation can affect results.
Sources
- Haemoglobin Testing Methods - news-medical.net
- Sahli's Method For The Estimation Of Hemoglobin - laboratorytests.org
AI-generated content may contain errors. Please verify critical information
