What is Barfoed's test and how does it differentiate between reducing monosaccharides and disaccharides?
Understand the Problem
The text describes Barfoed's test, which is a chemical test used to differentiate between reducing monosaccharides and disaccharides. It explains the conditions under which monosaccharides react in acidic medium and how they reduce cupric ions to form a precipitate, along with the reagents used in the test.
Answer
Barfoed's test differentiates monosaccharides and disaccharides based on their reduction rate of copper acetate in acidic medium.
Barfoed's test differentiates monosaccharides and disaccharides by using copper acetate in an acidic medium. Monosaccharides quickly reduce cupric ions to form red precipitate (Cu2O), while disaccharides react much slower.
Answer for screen readers
Barfoed's test differentiates monosaccharides and disaccharides by using copper acetate in an acidic medium. Monosaccharides quickly reduce cupric ions to form red precipitate (Cu2O), while disaccharides react much slower.
More Information
Barfoed's test is particularly useful for distinguishing simple sugars in biological and chemical analysis. It primarily detects reducing sugars by noting the speed and presence of a red precipitate.
Tips
Ensure the correct timing and acidic conditions are maintained during the test to get accurate differentiation between monosaccharides and disaccharides.
Sources
- Barfoed's Test - BYJU'S - byjus.com
- Barfoed's Test- Definition, Principle, Procedure, Result, Uses - microbenotes.com
- Barfoed's Test - Carbohydrates - Harper College - dept.harpercollege.edu
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